ICYMI: a quick calculation of transmembrane domain-containing proteins in a proteome.

If you need to use this info: the code is in the post, or you can cite it since all quantixed posts have their own DOI courtesy of Rogue Scholar. doi.org/10.59350/0ah...

doi.org/10.1038/s415...

🧵 Our new paper is out today in molecularcell.bsky.social! After 7 years of a truly intriguing discovery journey, we can finally share what controls how molecules move inside living E. coli cells. Spoiler: it's not what the field thought. A thread 👇
📄 doi.org/10.1016/j.molcel.2026.03.018

Life-like behavior emerging in active and flexible microstructures | PNAS Many organisms leverage an interplay between shape and activity to generate motion and adapt to their environment. Embedding such mechanical feedba...

www.pnas.org/doi/10.1073/...

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature Cas9 structures explain topology sensing and off-target activation.

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵

🔗 www.nature.com/articles/s41...

#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Please check out our newest work on fluorogenic speed imager probes, in which we combine the strengths of speed-optimized imagers and fluorogenic imagers.

#SuperResolution #DNAPAINT #microscopy

www.biorxiv.org/content/10.6...

DNA-PAINT with photocaged DNA labels = PhotoPAINT🔬. Why? No need for washing or mixing 👉🏻 higher accuracy in DNA-PAINT & other super-res methods.
Fantastic collab with Alex Heckel & driven by the 'magic team' Nina Kaltenschnee and Marina Dietz 💪
doi.org/10.1002/anie...
@goetheuni.bsky.social

β-barrel nanopores designed for insertion into thick block copolymer membranes www.biorxiv.org/content/10.64898/2026.03...

Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells - Nature Communications Spatially regulated membrane constriction is an important milestone in reconstituting minimal cell division. Here the authors engineer a truncated system where the cytosolic domain of FtsN forms large...

Neat paper from Schwille lab. They demonstrate constriction of synthetic vesicles using important components of the bacterial cell division machinery, FtsZ and FtsN. They induce strong constriction of Z-rings in vesicles just by adding the cytoplasmic domain of FtsN www.nature.com/articles/s41...

Molecular mapping in DNA-PAINT via modified Gaussian Mixture Modeling - Nature Communications Standard algorithms fail to fully exploit the spatial information in DNA-PAINT. Here, the authors present G5M, an algorithm providing molecular maps by accurately inferring biomolecule positions at di...

New paper alert! Authors introduce 𝗚𝟱𝗠, 𝗮 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧-𝗼𝗽𝘁𝗶𝗺𝗶𝘇𝗲𝗱 𝗚𝗮𝘂𝘀𝘀𝗶𝗮𝗻 𝗠𝗶𝘅𝘁𝘂𝗿𝗲 𝗠𝗼𝗱𝗲𝗹𝗶𝗻𝗴 𝗮𝗹𝗴𝗼𝗿𝗶𝘁𝗵𝗺 that accurately determines true molecular position, even at the 𝗥𝗮𝘆𝗹𝗲𝗶𝗴𝗵 𝗹𝗶𝗺𝗶𝘁, and 𝗶𝗺𝗽𝗿𝗼𝘃𝗲𝘀 𝗲𝗳𝗳𝗲𝗰𝘁𝗶𝘃𝗲 𝗿𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻 𝗯𝘆 ~𝟳𝟬%.

Huge congratulations to all authors!

www.nature.com/articles/s41...

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods 4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt

I was invited by @focalplane.bsky.social to write a short description about it:

focalplane.biologists.com/2025/12/12/a...

A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling.
www.biorxiv.org/content/10.6...

#HappyMicroscopyMonday #microscopycommunity-Join the AIMM user community on Friday, March 6, for a talk by Hannah Somers- “Stop Reinventing the Protocol: MicroHub, a Searchable Hub for Microscopy Knowledge”

Learn more: buff.ly/kZSIkSJ

Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating).

A brief 🧵 (carried over from the old place)

🙏 This study was powered by fantastic collaborations — especially with @abelljonny.bsky.social & @joechambers.bsky.social with contribution form @felixmendu.bsky.social @lasergroup.bsky.social @franbottanelli.bsky.social 🔬🧠

www.nature.com/articles/s41...

Continuous mass photometry by single molecule trapping www.biorxiv.org/content/10.64898/2026.02...

Ragaller, F., Sjule, E., Urem, Y. B., ..., Blom, H., & Sezgin, E. (2024). Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements. The journal of physical chemistry. B, 128(9), 2154–2167. #EpithelialMechanics
buff.ly/YjCWSfP

A protein called Uninflatable acts as a switch between glial cells growing and glial cells wrapping themselves around axons.

🔗 buff.ly/WRdybO3

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody.

Its binding turns on and off with a magnet.

This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

New paper from @zhixingchen2.bsky.social's lab!

It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!

www.nature.com/articles/s41...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos.
www.nature.com/articles/s42...

The new version of TrackMate, the v8.1 has just been released. It builds upon the recent v8, linked below, and offers two main improvements 👇

📢 Paper alert 📢

Chirality is known to be important for the movement of microorganisms and active matter. In our new paper out today in @natphys.nature.com, we show that chirality is used by malaria parasites to control their motion patterns:

doi.org/10.1038/s415...

Here comes a 🧵 ... (1/9)

StayRose: A photostable StayGold derivative redshifted by genetic code expansion Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence engineering, addresses thi...

Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡

Microglia modulate Aβ-dependent astrocyte reactivity in Alzheimer’s disease - Nature Neuroscience Microglia influence amyloid-β effects on astrocyte reactivity in the living brain of individuals with Alzheimer’s disease. This phenomenon further contributes to cognitive impairment via tau phosphory...

Just published in Nat. Neurosci.

Led by J.P. Ferrari-Souza, this study used imaging & fluid biomarkers to reveal that microglia modulate Aβ-related astrocyte reactivity, which in turn contributes to AD progression.

@erzimmer.bsky.social
@natureportfolio.nature.com

www.nature.com/articles/s41...

Language Models are Injective and Hence Invertible Transformer components such as non-linear activations and normalization are inherently non-injective, suggesting that different inputs could map to the same output and prevent exact recovery of the in...

I did not expect that: Large Language Models are invertible:

arxiv.org/abs/2510.15511

Figure showing that tagged tubulin is frequently incorporated into the OF tip in cells that are in G1-phase.

Daniel Abbühl, Philippe Bastin @bastinlab.bsky.social and colleagues use a novel approach for tagging tubulin to dissect microtubule assembly dynamics of the axoneme in Trypanosoma brucei.
#JCSciliaSI
journals.biologists.com/jcs/article/...

Sub-10 nm upconversion nanocrystals for long-term single-particle tracking - Nature Communications This study presents ultrasmall, bright lanthanide-doped upconversion nanoparticles that overcome surface quenching, enabling high spatiotemporal resolution tracking of membrane proteins with exception...

www.nature.com/articles/s41...

Using super-resolution microscopy to investigate the mechanistic principles of bacterial cell wall remodelling at University of Warwick on FindAPhD.com PhD Project - Using super-resolution microscopy to investigate the mechanistic principles of bacterial cell wall remodelling at University of Warwick, listed on FindAPhD.com

PhD project available in my lab. Email me with CV if interested
www.findaphd.com/phds/project...
#microsky #bioimaging