a man in a white shirt and tie is standing in front of a poster that says new york city . ALT: a man in a white shirt and tie is standing in front of a poster that says new york city .

Pretty sure I came across the GitHub for that the other day, I’ll have to check it out further. I also think I might have got too excited too soon because I spent the last hour learning firsthand how much timsControl likes to lag

a man is looking through a futuristic screen with the number 3000 on it ALT: a man is looking through a futuristic screen with the number 3000 on it

How I feel editing my first DIA-PASEF polygon

Chemoproteomic Elucidation of β-Lactam Drug Targets in Mycobacterium abscessus The pathogen Mycobacterium abscessus (Mab) can cause severe and difficult-to-treat chronic lung infections. Despite the rising incidence and clinical concern of Mab infections, treatment options are l...

New work from my team: pubs.acs.org/doi/10.1021/...
We used probes to ID targets of carbapenems in M. abscessus. 17 targets active in both carbon starved and replicating cultures. Includes validation of 9 targets. Part of a special issue ("NTM Pathogens – Biology, Infection, and Drug Discovery).

Is there a word that encompasses the class of enzymes that act on glycoproteins? Glycoproteases indicates a specific proteloytic activity, and I’d like something more akin to CAZymes that includes non-hydrolytic enzymes.

#glycotime

Postdoc Position in Chemical Biology Join us to design cutting-edge chemical biology tools and reveal molecular mechanisms in complex biological systems.

We have a 3-year post-doc position open. Apply now and join BioBeyond_NL to power up mass spec studies with new chemical biology tools!

www.uu.nl/en/organisat...

Come join us a #bioinformatics / #computationalbiology postdoc to explore the #microbiome using #metaproteomics and #metagenomics

Microbiome Bioinformatician (Postdoc) - Metagenomics and Metaproteomics @RalserLab
Charité Universitätsmedizin Berlin

See the full job description on jobRxiv: https://j

Impact of inactivation methods on biosafety and antigen reactivity of Brucella melitensis from the perspective of Astral-DIA proteomics based on antibody immunoprecipitation mass spectrometry Author summary Brucellosis is a widespread zoonotic disease that poses significant health risks to both humans and livestock globally. To study this dangerous bacterium safely—and to develop vaccines ...

Welcome to the battle against Brucellosis disease: Field isolates proved more resistant than lab strains, req. stricter conditions (95°C or 0.6% formaldehyde). Proteomics reveals why: heat exposes epitopes (“cooking”), formaldehyde preserves structure (“freezing”).

journals.plos.org/plosntds/art...

This is the coolest paper I’ve seen in a while. The future of mass spec is bright

Interested in Activity-based Protein Profiling?

We are excited to host the 15th Activity-based Protein Profiling meeting (@abpp2027.bsky.social #ABPP2027) on June 2nd to 4th, 2027, at Naturalis in Leiden.

Save the date and sign up for our mailing list: www.universiteitleiden.nl/en/science/l...

Great opportunity to work as a #PhD or #postdoc with a great PI (@mlakemeyer.bsky.social), at @uni-jena.de.

Interested in #ChemBio, #Biochemistry, #Chemistry, #Microbiology and/or pharmaceutical sciences? Check it out:

PhD: jobs.uni-jena.de/jobposting/8...
Postdoc: jobs.uni-jena.de/jobposting/c...

O-Glycans next plz

GLYCO-BUILD, an entirely enzymatic approach for the production of N-glycans and N-glycopeptides.

www.nature.com/articles/s41...

This view resonates deeply with me:

⬛ Publish fewer claims and more proof

Papers need to include fewer claims and more proof to make the scientific literature more reliable

What is driving today’s ‘claims inflation’?

1/3

Does anyone know of good conformation prediction software for PTMd peptides? There’s lots of good resources for unmodified peptides but many do not include PTMs, or extremely limited. Specifically interested in glycosylation.
#glycotime
#BioML

Ninhydrin as a covalent warhead for chemical proteomic-enabled discovery and selective engagement of reactive arginines Covalent molecules have emerged as next-generation therapeutics and as powerful tools for perturbing fundamental biological processes. Chemical proteomic methods to screen for reactive proteinaceous a...

Is arginine the new cysteine?! Check out our lab's latest in collaboration with @ianseiple.bsky.social's team where we introduce ninhydrin as a selective covalent warhead and probe targeting reactive arginines.
www.biorxiv.org/content/10.6...

Read an interesting paper on how vWF sialylation essentially increases its half-life by preventing lectin-mediated clearance from the blood stream. I wonder if there’s other examples of glycosides acting as time-gated PTMs to proteins, where “rare” glycosides = longer half-life. #glycotime

My relationship with immunology is basically represented by Stockholm syndrome.

Discovering Glycosylation-Dependent Protein Function by Thermal Proteome Profiling Protein glycosylation regulates essential cellular processes including protein folding, stability, and cell-cell interactions; however, how aberrant glycosylation impacts protein function and interact...

Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏

www.biorxiv.org/content/10.6...

Had a fantastic time at the New Horizons in Drug Discovery conference put on by @thewelchfoundation.bsky.social - also loved this slide from @dschweppe.bsky.social, sums me up pretty well

You’ve heard of #Lymedisease, but have you ever seen the bacteria that causes it? 🦠 Borrelia burgdorferi, the spiral-shaped culprit transmitted by ticks, swims with a creepy wriggling motion and uses #chemotaxis to navigate. Both fascinating... and disgusting #Microsky 🧪 #IDsky @shawlab.bsky.social

Next week, Scripps Research scientists Amy Lightner, Michael Bollong & Arnab Chatterjee will speak at #IBDCuttingEdge2025, sharing insights on regenerative medicine, clinical trials, and drug discovery in IBD.

Join us next week, Sept 19–21 | 📍 Coronado, CA
🎟️ Register now: ow.ly/xPsK50VFU8L

Ruin any book by adding "Tom Clancy's" to the title.

Tom Clancy's Protective Groups in Organic Synthesis

Gas Phase Separation of Modified Peptides for Activity-Based Protein Profiling Profiling the reactivity of the proteome with amino-acid level resolution requires the identification and quantification of reacted peptides in the presence of abundant unmodified peptides. Affinity-b...

An inspiring paper by the group of Jacob Geri in Analytical Chemistry. They present a method for residue-specific #chemoproteomics without the need for an enrichment step. To achieve this, they developed reagents that shift the ion mobility for gas phase separation. pubs.acs.org/doi/full/10....

Ultra-sensitive metaproteomics redefines the dark metaproteome, uncovering host-microbiome interactions and drug targets in intestinal diseases www.nature.com/artic...

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#proteomics #prot-paper

Probing the proteome Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?

Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. A feature in Nature Biotechnology explores whether it can deliver a new generation of drug targets. 🧪

PNGaseL from Flavobacterium akiainvivens targets a diverse range of N-glycan structures PNGases are used by a wide range of organisms to remove N-glycan structures from proteins for use as either nutrients or in glycoprotein processing. PNGaseF is the most well-characterised enzyme of th...

Very pleased to present the next pre-print from our lab #glycotime We showcase PNGaseL from Flavobacterium akiainvivens that we hope will be a useful tool for the glyco community 🍬🧪👩‍🔬 Special shout out to @cassiebakshani.bsky.social and Paulina (Ludger)
www.biorxiv.org/content/10.1...

Biblically accurate nitrogen

A "cross-decoupling" or "retro-Suzuki" sequence

Fans of Guenther Schlonk will enjoy the "cross-decoupling" easter egg:

jabde.com/wp-content/u...

Checking USCSB quarterly is one of my favorite hobbies

Are you really a synthetic chemist if you aren't watching USCSB videos?