Multiplex immunofluorescence of Aβ species
🚨New in Lancet Neurology: Autopsy study of aducanumab in #Alzheimer’s shows superficial cortical Aβ cleared, but deeper persists. ARIA showed inflammation+ microinfarcts in leptomeningeal/penetrating vessels. Some path is PET-invisible. @drneurochic.bsky.social
authors.elsevier.com/a/1lxn85FFzL...
Replace paraffin embedding for frozen tissue sectioning: The process of fixation, dehydration, and infiltration with paraffin can damage or destroy some crucial antigens. Avoid working with mounted slide and use free-floating sections: Allows antibodies and other reagents to penetrate the tissue from all sides, especially when combined with overnight incubation in a shaker. Thin vs. thick sections : What do you want to analyze? Thicker sections will allow more complex 3D reconstructions and blood vessel analysis. DAB vs. immunofluorescence (IF): IF will produce less background and allows studying co-localization of multiple targets
What can you do to improve your microscopy images?
I often receive questions regarding how to take better images, so let’s start with the basics: improve your sample preparation! No microscope can make a miracle if your samples don’t look great! Things I don’t do anymore: (1/5)
🎉 @istaart.bsky.social #AtypicalAD PIA Executive Committee is elected!
Chair: Keir Yong
Vice Chair: @baaylab.bsky.social
Programs Chair: Rosaleena Mohanty
Communications Chair: Colin Groot
Early Career Representative: @sararosedunlop.bsky.social
Immediate Past Chair: @rikossenkoppele.bsky.social
Interested to see how advanced microscopy holds the power to transform our understanding of therapeutics🔬🧠…come see our latest work. #AAIC25
Hall G session 2-3.30 pm integrative neuropathology in ADRD at 2.20 pm sharp
@drneurochic.bsky.social
📢 Excited to announce the lineup for the @istaart.bsky.social #AtypicalPIA FRS at #AAIC25 on July 29! Mark your calendar to join us in person or online with @neusfalgas.bsky.social @leatgrinberg.bsky.social @rikossenkoppele.bsky.social @baaylab.bsky.social Drs. Deepti Putcha, Keir Yong, Colin Groot!
At #ADPD2025?? Go check today&tomorrow Sarah Basile at poster 174 on #amyloid-beta nitration and its role in fibrillation and #neuroinflammation in #Alzheimer. We would LOVE to hear your thoughts!
Kicking off #CONY2025 in style w/ debate: "Should atypical #Alzheimer be included in trials?" What do you think? discussion w/ @rosaleenam.bsky.social @keiryong @rikossenkoppele.bsky.social & Judith Aharon & support @alzassociation.bsky.social @istaart.bsky.social
Excited to share our findings on how APOE genotype impacts cell responses to AD using human brain snRNAseq. Huge thanks to the team for all the hard work! @drneurochic.bsky.social @mayoclinic.org @cp-neuron.bsky.social
Studying the proteome of amyloid-beta plaques in Alzheimer's disease without the need to perform immunohistochemical staining? Sounds like science-fiction but now possible thanks to this great collaborative project!!
@baaylab.bsky.social @axlmsg.bsky.social #proteomics #alzheimers 1/3
By going beyond ‘Atypical’ / ‘AD’ @jeffandthebrain.bsky.social got me excited about interesting unexplored avenues. And! A whole lot of relevant papers I still need to read. Check the recording @istaart.bsky.social available in ~3 weeks if you missed it!
Do not forget to join us for the @istaart.bsky.social Atypical AD PIA #YearinReview webinar today! The webinar will be led by @jeffandthebrain.bsky.social followed by a discussion with @rikossenkoppele.bsky.social @baaylab.bsky.social & Colin Groot!
Register here:
alz-org.zoom.us/webinar/regi...
Wow!!!😍
And I from you! Such a pleasure to be on stage with you 🥳🥳🥳
So glad I finally made it to #HAI25! It was amazing to show our data, which would not be possible without the amazing collaborative infrastructure of the Mayo Clinic ADRC #neuropathology
#NIHAging