#cryoET #TeamTomo

The image below shows a slice through a tomogram of the human sperm nuclear envelope (NE) and part of the connecting piece with the segmented columns (SC). Within the double membrane of the NE, you can see the regular protein densities of the LINC lattice (highlighted by yellow arrows).

These insights provide an explanation for the devastating phenotype observed in sperm of patients with SUN5 mutations, mainly characterized by a detachment of sperm head and tail and consequently resulting in infertility.

Our data suggests that SUN5, a protein involved in the formation of LINC (Linker of nucleo- and cytoskeleton) complexes, is forming this lattice.

In this study, we combined fluorescence microscopy, in situ cryo-ET and structure prediction tools to the mammalian sperm connecting piece and reveal the structure of a protein lattice in the nuclear envelope of human, mouse and boar sperm.

Super happy to see our paper on the 🔗LINC lattice in mammalian sperm in its final form: doi.org/10.1073/pnas....

Thanks to everyone involved @tzviyazbm.bsky.social, Svetlana Doroshev and Miguel Leung!

33 million euro investment for national electron microscope facility EMPower A major new national facility for electron microscopy, called EMPower, has been awarded to a consortium of Dutch universities and medical centers. EMPower aims to pave the way for major discoveries im...

Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that!
www.uu.nl/en/news/33-m...

#hiring #researchassociate #structuralbiology #proteinsciences #cryoem #biochemistry #biophysics #dnarepair #drugdiscovery | Stanislau Yatskevich We’re hiring a 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐀𝐬𝐬𝐨𝐜𝐢𝐚𝐭𝐞 (Contractor) to join our 𝐒𝐭𝐫𝐮𝐜𝐭𝐮𝐫𝐚𝐥 𝐁𝐢𝐨𝐥𝐨𝐠𝐲 team in 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 𝐒𝐜𝐢𝐞𝐧𝐜𝐞𝐬 focused on the molecular mechanisms of DNA repair. 𝐖𝐡𝐲 𝐭𝐡𝐢𝐬 𝐫𝐨𝐥𝐞 𝐦𝐚𝐭𝐭𝐞𝐫𝐬 You’ll help purify and ...

my friend and wonderful colleague Stan is hiring at Genentech in South San Francisco! 🧬 🌉

Biochemist/structural biologist looking for your first position in industry? I can’t recommend working with Stan enough ☺️ please repost for visibility

www.linkedin.com/posts/stanis...

Cryo-EM single-particle analysis expanding towards increasingly native samples Cryo-EM single-particle analysis has revolutionized structural biology by allowing high-resolution analysis of large molecular assemblies that are not amenable to crystallography or NMR. Combination of this technology with innovative sample-preparation protocols further expands the range of potential targets.

Jonas Moecking et al.: Cryo-EM single-particle analysis expanding towards increasingly native samples #SingleParticleAnalysis #InSitu #CryoEM @uniutrecht@uniutrecht...#IUCr https://journals.iucr.org/paper?S2059798325008332

Happy to see this out :)
How close does single-particle cryo-EM get to in situ structure determination? In this review, @tzviyazbm.bsky.social and I explore recent examples of studies that we believe push the boundaries of what's possible. #cryo-EM

Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/

SUN5 forms a regular protein lattice reinforcing the sperm head-tail junction Linker of nucleo- and cytoskeleton (LINC) complexes reside in the nuclear envelope, the double-membrane surrounding the nucleus, where they establish a physical bridge between nucleus and cytoplasm. L...

I am happy our recent study on the human sperm head-tail junction is now online. We find that SUN5 form an hexagonal lattice at the nuclear membrane, rationalising how mutations in SUN5 lead to infertility. Great work of @cryonas.bsky.social !
www.biorxiv.org/content/10.1...

Last but not least I would like to thank everyone involved! Quite excited to join #teamtomo! 4/4

SUN5 forms a regular protein lattice reinforcing the sperm head-tail junction Linker of nucleo- and cytoskeleton (LINC) complexes reside in the nuclear envelope, the double-membrane surrounding the nucleus, where they establish a physical bridge between nucleus and cytoplasm. L...

Using subtomogram averaging we further determine the structure of the SUN5 LINC lattice and propose a model in which the lattice, formed by SUN5 trimers, reinforces the head-tail junction. This model could also explain SUN5's role in ASS. Full story here: www.biorxiv.org/content/10.1... 3/4

SUN5 is part of LINC (linker of nucleo- and cytoskeleton) complexes, which do exactly what their name suggests: physically link🔗 the nuclear lamina with the cytoskeleton via the NE. Specifically staining for SUN5, we confirm its localization to the posterior sperm head by fluorescence microscopy.2/4

ASS is short for acephalic spermatozoa syndrome in which head and tail of sperm are detached⛓️‍💥, commonly caused by mutations in the gene encoding for SUN5. Using in situ cryo-ET❄️🔬 we find that SUN5 forms a protein lattice in the nuclear envelope (NE) at the head-tail junction of mammalian sperm.1/4

Credit: Margot Riggi (@margotriggi.bsky.social), Max-Planck Institute of Biochemistry (@mpibiochem.bsky.social)

Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6

Mature #HIV-1 contains >2000 copies of “spacer peptide 2”, but we didn’t know why. We now see that SP2 binds the matrix protein and triggers the change into its mature arrangement. Summary video from @margotriggi.bsky.social