GLI1 Coamplification in Well-Differentiated/Dedifferentiated Liposarcomas: Clinicopathologic and Molecular Analysis of 92 Cases - PubMed GLI1(12q13.3) amplification is identified in a subset of mesenchymal neoplasms with a distinct nested round cell/epithelioid phenotype. MDM2 and CDK4 genes are situated along the oncogenic 12q13-15 se...

pubmed.ncbi.nlm.nih.gov/38621503/

This is classic morphology for a GLI1 coamplified differentiated liposarcoma with perineurial-like whorls. So now the MDM2 amplification makes sense. The current tumor is showing essentially classical features of a GLI1 amplified soft tissue sarcoma. So the morphology/molecular explain each other.

#PathSky Laryngeal mass, older adult male. Primitive, highly vascular round cell neoplasm for the most part.

STAT6 Can Be Used in the Diagnosis of Hodgkin Lymphoma;... : Applied Immunohistochemistry & Molecular Morphology the STAT6 (EP325) immunostain, diagnosing Hodgkin lymphoma, its staining frequency, immunolocalization, and its relationship with EBV. Sixty-eight patients representing each Hodgkin lymphoma subtype w...

STAT6 Can Be Used in the Diagnosis of Hodgkin Lymphoma; There Are Differences in Expression Among Subtypes journals.lww.com/appliedimmun...

Distinct levels of DDIT3 Amplification in Dedifferentiated... : Applied Immunohistochemistry & Molecular Morphology diagnostic tool for accurate diagnosis. While DDIT3 rearrangement is a known characteristic of myxoid LPS, its amplification in DDLPS has not been extensively studied. To investigate this, we evaluate...

Distinct levels of DDIT3 Amplification in Dedifferentiated Liposarcoma Developing After DDIT3 Rearrangement in Myxoid Liposarcoma journals.lww.com/appliedimmun...

Investigating Biomarkers in Primary Tumors and Lymph Node... : Applied Immunohistochemistry & Molecular Morphology tases. A total of 60 TSCC biopsy samples, 20 primary metastatic, 20 lymph node metastatic, and 20 nonmetastatic, were immunohistochemically stained to analyze MCM2, CD44, and E-cadherin expression. Ex...

Investigating Biomarkers in Primary Tumors and Lymph Node Metastases in Tongue Squamous Cell Carcinoma: An Immunohistochemical Perspective journals.lww.com/appliedimmun...

Nasopharyngeal mass, adult male.

Dx: Hyalinizing clear cell ca.

➡️Nests of eosinophilic and/or clear cells. Helpful: 2 ‘types’ of fibrosis (dense hyalinization and fibrolamellar stromal fibrosis).

#PathSky #ENTsky #HeadNeckSky

Tracheal adenoid cystic carcinoma. At high magnification (next 2 pics), areas of ‘signet-ring like’ or vacuolated, clear cell change is noted in ducts. Unknown clinical significance but when present in small biopsy material, may lead to diagnostic uncertainty.

PMID: 23379326
PMID: 39235305 (fig 3)

Image of the Week!

Submitted by: Halida Hakic Beslagic, M.D.
Directory Profile: buff.ly/ys2Oh0O
Subspecialty: GI / liver
Diagnosis: FNA Liver - adenocarcinoma

#Pathology #PathologyOutlines #PathSky

Just published! Check out this latest article 👉🏻 HER2 amplification is rare (1.7%) in low-grade, tubule-forming breast cancers with 2+ HER2 IHC; findings suggest ISH may be unnecessary in this subset #breastpath #IHCpath #pathsky

All that palisades etc etc.Seen an endometrial stromal sarcoma with crazy palisading just like this.

#PathSky Middle aged woman, leg mass. It all looks like this. More nuclear palisading/ Verocay bodies than you’ve seen in your entire career. Nerve sheath? S100/Sox10 negative. LMS? Negative for all the muscle markers. Any other ideas?

ROFL 🤣

Desmoid Fibromatosis With TP53 Mutation and Striking Nuclear Pleomorphism - PubMed Desmoid fibromatosis is a myofibroblastic neoplasm of intermediate biologic potential, which has a strong predilection for local recurrence but does not metastasize. Arranged in long, sweeping fascicl...

So- desmoid fibromatosis with bizarre cells and p53 mutant pattern- r/o Li-Fraumeni syndrome.

Happy Friday!

pubmed.ncbi.nlm.nih.gov/36908221/

Beta-catenin

#PathSky Abdominal wall mass, middle aged woman. Women basically only get 3 abdominal wall tumors, so it’s got to be a desmoid, DFSP or endometriosis. Classic desmoid morphology, aberrant nuclear beta-catenin. I can hear the cries of “boring..”

Beautiful MIFS thanks.

This of course is what we now call “myxoinflammatory fibroblastic sarcoma”. Originally reported as “Inflammatory myxohyaline tumor of distal extremities with virocyte or Reed-Sternberg-like cells”. I think this older name helps us remember the distinctive diagnostic features of this sarcoma.

#PathSky 43M, forearm mass. At first glance, it looks like some kind of inflammatory process, maybe a synovitis with plasma cells, fibrosis and foamy macrophages.

Degenerating vegetable. Likely a ruptured diverticulitis with a big reactive soft tissue mass. Happy Friday!!

Those of you following my cases know the answer is usually “look at the slides some more”.

#PathSky 45 with a forearm skin tumor. Highly cellular, monomorphic spindle cell tumor with a “packeted” pattern and deep, nodular lymphoid aggregates.

Check out our Current Issue!!

Figure from "Claudin 18.2 Immunohistochemistry Expression in Gastric Cancer: A Systematic Review” by Lop Gros, et al.

🔗 Read the full article here: journals.lww.com/appliedimmun...

Awesome!

Incredible "Schwannomatoid" pattern in a monophasic synovial sarcoma. #skypath #pathsky #BSTPath #Sarcoma

Thoracic Pathology Unknown Slide Conference Feb 2019 YouTube video by Sanjay Mukhopadhyay

youtu.be/4i1mnz889bo?...

Vaccines don’t cause autism.
Vaccine ingredients don’t cause autism. Vaccines don’t worsen autism symptoms.

Millions of data points over decades show this.

A paper published in a “journal” that funded the “study” doesn’t supersede reality.

Stop spreading lies that harm people.

1/

www.sciencedirect.com/science/arti...
Link to the paper that should help everyone diagnose keratoacanthoma with greater confidence.

Recurrent novel HMGA2-NCOR2 fusions characterize a subset of keratin-positive giant cell-rich soft tissue tumors - PubMed Giant cell tumors of soft tissue (GCT-ST) are rare low-grade neoplasms that were at one time thought to represent the soft tissue counterparts of GCT of bone (GCT-B) but are now known to lack the H3F3...

pubmed.ncbi.nlm.nih.gov/33742141/

#pathsky

Xanthogranulomatous Epithelial Tumors and Keratin-Positive Giant Cell Rich Tumors of Soft Tissue and Bone: Two Sides of the Same Coin - PubMed Xanthogranulomatous epithelial tumor is a recently described soft tissue tumor characterized by subcutaneous location, partial encapsulation, a xanthogranulomatous inflammatory cell infiltrate, and ke...

We eventually figured out that this was a new entity, “xanthogranulomatous epithelial tumor”. Locally aggressive, non-metastasizing. They turn out to have unique HMGA2::NCOR fusions. “Keratin-positive giant cell tumor of bone” is the same thing. pubmed.ncbi.nlm.nih.gov/38278607/

#PathSky It’s interesting how we discover things sometimes. On this particular Sunday, I got an email from an Ortho Onc asking what a JXG was. “Just a benign skin tumor.” “Well, this is deep and eroding bone.” “Erm, let me look at it…” Mostly looks like JXG, but what are those eosinophilic cells?