Uncovering the transcriptional hallmarks of endothelial cell aging via integrated single-cell analysis Endothelial cells (ECs) are critical regulators of vascular function and exhibit specialized, organ-specific roles across tissues. During aging, these cells become dysfunctional, resulting in increase...

Uncovering the transcriptional hallmarks of endothelial cell aging via integrated single-cell analysis
www.biorxiv.org/content/10.1...

We hope our atlas can go beyond and become a framework to explore how #vascular #aging unfolds across the human body. Explore the data yourself at haeca.derooijlab.org, and check out the rest of the story here: biorxiv.org/content/10.1.... And importantly, let us know what you think! 9/9

Led by Sarah Dobner, this is the very first derooijlab.org production, and we want to thank all lab members and collaborators for their contributions, our host institution @cemm.oeaw.ac.at, as well as our funders. 8/9

5️⃣ Lastly, by integrating the atlas data with in vitro models of induced EC senescence, we uncovered a core set of genes linked to CDKN1A/p21 expression. We validated this across models, tissues and species, to further our understanding of senescence regulation in ECs. 7/9

4️⃣ Age-associated ECM remodeling signatures were linked to increased activity of #mechanotransduction regulators YAP/TAZ and TEAD, particularly in skin, lung, and liver. Immunofluorescence confirmed increased nuclear YAP in aged human skin ECs. 6/9

3️⃣ Venous aging is different:
Vein ECs lose expression of multiple angiogenic regulators with age, while increasing genes linked to extracellular matrix (ECM) remodeling and altered glycosylation, hinting at effects on #angiogenesis, barrier function, and immune trafficking. 5/9

2️⃣ Inflammaging is everywhere:
Across tissues, aging ECs showed increased expression of inflammatory signaling, immune cell adhesion markers, and #senescence-associated factors. 4/9

1️⃣ A human vascular “Rosetta Stone”:
We identified conserved molecular markers for each endothelial subtype across tissues. These can be used to pinpoint arterial, venous, capillary, or lymphatic ECs in any healthy human tissue context. 3/9

By integrating and harmonizing endothelial cells (ECs) from 34 publicly available #single-cell and single-nucleus #RNAseq datasets, we found 🧵(2/9):

🚨Preprint alert!🚨What happens to our blood vessels as we age? To begin answering this at the transcriptome level, we built the Human Aging Endothelial Cell Atlas (HAECA), profiling >375,000 endothelial cells from 12 tissues across adulthood. www.biorxiv.org/content/10.1... (1/9)

#BookSky

The innovation trade-off: how following superstars shapes academic novelty - Humanities and Social Sciences Communications Humanities and Social Sciences Communications - The innovation trade-off: how following superstars shapes academic novelty

"our results indicate that academia pays a price by focusing attention and resources on superstars"

Nice to see this supported by data analysis, now.

H/T: @cxdig.bsky.social

www.nature.com/articles/s41...

🔬 As we near the end of #EquationsForLife, today we spotlight @ar-abdelfattah.bsky.social's Group!
They use the #ReactionDiffussion equation to characterize the process of tissue organization and maintenance in physiology, and how disease disrupts it.
🔎 bit.ly/4ef2Pbe

We kicked off the week with Roger Kamm (@mitdeptofbe.bsky.social) for a #CeMMinar on brain neurovascular models & its applications to modeling drug & toxin transport. Thanks to Roger for the great insights & hosts @lderooij.bsky.social & @ar-abdelfattah.bsky.social

A very insightful Impromptu Seminar today at #CeMM by Kumar Sharma (@uthealthsa.bsky.social), hosted by our PI @ar-abdelfattah.bsky.social! His talk on spatial mass spectrometry & precision therapeutics offered powerful insights into disease mechanisms and inter-organ crosstalk. Thank you, Kumar!

🌐 Introducing MOTION WorkPackage 2 by @reibergerthomas.bsky.social 🌐

In this particular WP the team is focusing on decompensated cirrhosis..
#liver #PortalHypertension #clinicaltrails #dACLD

🔽 Find out more in the video below 📷🔽

Let’s kick off the week with #EquationsForLife! @lderooij.bsky.social’s group studies how #aging affects endothelial cells in blood vessels. Using #MurraysLaw & 3D “vessel-on-a-chip” models, they aim to decode & combat cardiovascular aging.
🔍 tinyurl.com/CeMMRR2024-d...
#CeMMResearchReport2024

🚨 Exciting Opportunity! 🚨
Join us on June 9th in Brussels for a satellite conference to the 4th MPS World Summit, where we will address advancements & challenges in #aging research using #microphysiological systems and #organoids.
Sign up here: lnkd.in/dCu_yCBr

All of this in a super supportive research environment in one of the world's most liveable cities!

For further details and to apply, please follow the link below: lnkd.in/d_K4NdJA

Check out our website for more info about our group and ongoing projects: derooijlab.org

🚨Job alert🚨! We are looking for a highly motivated research technician to join us in our quest to dissect the transcriptomic make-up and function of endothelial cells across the human body, using cutting-edge omics technologies, clinical samples, and vessel-on-chip models. Please share & apply! 👇

Only two weeks left to apply, don't miss out!! 👇

Excited to share our single-cell study on aging mouse brain! We found 2,449 cell-type specific age-DE genes, and an aging hotspot in specific hypothalamic cell types involved in energy homeostasis, which show both decreased neuronal function and increased immune response. doi.org/10.1038/s415...

Learn more about our research: derooijlab.org
Find out how to apply: cemm.at/phd-program

We make use of state-of-the-art #omics technologies and human in vitro models, including exciting #vesselonchip systems. Wet & dry lab enthusiasts are thus both very welcome to apply! Relevant links 👇

Looking for a fully funded PhD position in wonderful Vienna? The CeMM PhD program is now open for applications! 🎓 🥼 🧫

We are seeking passionate and driven students interested in helping us understand #endothelial cell heterogeneity in both healthy and pathological #aging. See below for more info 👇

Is anyone else just *not interested* in using AI for their writing? All accuracy and morality aside, I just don't want to. I want every last word I put on a page to have flowed out from my own body. Maybe I'm romanticizing it, but I just can't see it any other way.

Our #TBI project got funded! Driven by what happens when forces are too much to handle, we will engineer tools to traumatize organoids and focus on #ECMatrix dynamics, #mechanobiology, and molecular-to-multicellular tissue organization. Happy to collaborate! Happening @ CeMM

High content of nuclei-free low-quality cells in reference single-cell atlases: a call for more stringent quality control using nuclear fraction - BMC Genomics The advent of droplet-based single-cell RNA-sequencing (scRNA-seq) has dramatically increased data throughput, enabling the release of a diverse array of tissue cell atlases to the public. However, we...

Not checking nuclear markers like MALAT1 or intronic reads in your scRNA-seq data?🚨
We show their power to flag low-quality cells—even in top public datasets. It’s time to prioritize better QC for cleaner, more reliable genomics research!
Read more: bmcgenomics.biomedcentral.com/articles/10....
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I would love to be added! :)

The complications of SARS-CoV-2 infection highlight the key role of #endothelial functions in health and disease. Great perspective in light of acute and Long Covid, and strongly in line with what we previously speculated: doi.org/10.1161/CIRC...