#FijiImageJ macro time!
It's been a while...but somehow it feels like riding a bike.
#microscopy analysis #bioimaging #organelles
Posts by Pablo J. Sáez
Fantastic paper !
What *is* surprising is how good it is - don't think anyone ever saw more accurate, direct macrophage chemotaxis. The movies are remarkable.
The device on the left has no initial gradient, just homogeneous C5a throughout. The macrophages create the gradient themselves, while they go.
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How do proteins find their way to the cell's leading edge? @jimgalbraith27.bsky.social & @cathygalbraith.bsky.social break down how bleach line, FLOP, single-particle tracking, FCS & iPALM reveal intracellular fluid flow and an actin-myosin compartment barrier. doi.org/10.1038/s414...
Just because it's Monday, we love #microscopy & we love #mitochondria, the powerhouse of the #cell
We videoed mito under the microscope to see their dynamics.
Here they join forces!
Ofc we <3 the other #organelles too (to prevent jealousy)
From @cellcommlab.bsky.social 🧪🔬 @focalplane.bsky.social
Good luck @andreamurillo.bsky.social !
Thanks ! This Flip looks amazing, I'll check if there's anything available for calpain.
You da best @joachimgoedhart.bsky.social !
Yes !! That's what I meant...single FP based reporter for calpain
Sorry, I wrote the previous message while doing other stuff and it's practically impossible to make any sense out of it.
Cool ! Do you know any good single fluorescence reporter?
Our latest #preprint list focusses on new research in bioimage analysis and data management.
Drop us a message if you have a suggestion for this list, or our lists on new tools and techniques in imaging or applications of microscopy in biology.
focalplane.biologists.com/2026/03/20/m...
We’re hiring!
Our lab is seeking a research technician interested in neurobiology. This position is ideal for recent graduates looking for a 2‑year research experience before their next academic chapter. Experience with Drosophila is a plus.
Please share.
indiana.peopleadmin.com/postings/32602
Working with amazing people in an amazing place @johninnescentre.bsky.social , and getting to see hundreds of these videos was already beyond my wildest dreams... Now surprised and happy to see that the wave of calcium joy keeps travelling! :)
🎉 Registration OPEN! 🎉
The 3rd yEFIS Symposium is now open for registration!
📍 Prague | 📅 29 June – 1 July 2026
✨ Keynotes • Talks • Posters • Workshops • Networking
📝 Abstract submission open
👉 Register via QR code or at yefis.org
#yEFIS2026
Correct, the changes in the fluo are only due to the mechanical damage.
Maybe @annalisabellandi.bsky.social could tell us more ;)
Quickly turning on the lights to do the cut?
Surely @annalisabellandi.bsky.social can tell us ;)
This is why we love #CalciumSignaling
Look how mechanical damage triggers long range Ca2+ waves in this plant !!
By @annalisabellandi.bsky.social, who is now around here ;)
Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬
Here is our updated database of grants for early careers researchers in all fields.
It goes way beyond traditional NIH and NSF funding opportunities. We list 428 types of grants.
Download it here: research.jhu.edu/rdt/funding-...
Neural crest cells beginning their long trek out of the neural tube in a quail embryo. Beautifully captured by the fantastic @siewzhuan.bsky.social 🧪🐣🔬
Visit our fully accessible journal archive, providing perpetual access to 34k articles from over a century of pioneering research in developmental biology, cell biology, comparative physiology and biomechanics, including work from 60+ Nobel Prize-winners bit.ly/3PfIjib
📢New behind the paper story
Using the #zebrafish retina as a model, Jaakko Lehtimäki
@jalehtimaki.bsky.social, Caren Norden
@nordenlab.bsky.social & team dissect multipolar migration in the developing brain. In this post, Jaakko takes us through their research.
This week we are introducing neuromodulatory assembloids.
Over the past few years, many circuits and cell-cell interactions have been modeled in assembloids, but these systems have not systematically incorporated neuromodulation.
Wow, it looks fantastic !!
How many tiles for this stitching? 😱
You are the best !!! Very much looking forward to collaborate !!!
Eres una crack 💪🏽🔥💪🏽
To be properly celebrated in the best place of all: Zé... 😉 (I cannot say the full name to keep it protected as @christlet.bsky.social & @henriqueslab.bsky.social said)
Our #preprint list is now up on FocalPlane.
Start your week feeling inspired by the latest research using microscopy to answer questions in biology. Let us know if you have any recommendations for us to add.
focalplane.biologists.com/2026/03/09/m...
#Science #Physique
🔬 « Transport dans la cellule »
🖥️ Le cours du Pr Jean-François Joanny, titulaire de la chaire Matière molle et #biophysique, est terminé... mais toutes les vidéos sont à re(voir) sur notre site web !
👉 https://tinyurl.com/3wfjve5c
New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...
2nd- or 3rd-year PhD student? Applications for @hhmi-science.bsky.social's #GilliamFellows Program open 9/1! Robust dissertation support, an incredible community of scientists, tailored professional development (for you *and* your faculty thesis advisor), & so much more. Bookmark bit.ly/4qQArRh!
You are finishing your PhD and looking to continue in science?
The Martin lab @biology-unige.bsky.social has an open postdoctoral position in cell biology to study cell-cell fusion. For more information, please consult mocel.unige.ch/research-gro....
Thanks for reposting!
Léa Marpeaux, Claire Baudouin, Gregory Emery @emery-lab.bsky.social and colleagues @umontreal.ca identify an apical supracellular actin network in epithelial cells that acts as a long-range force transmission device.
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
🔬🔦In our latest ‘Imaging spotlight’ Estibaliz Gómez de Mariscal @gomez-mariscal.bsky.social & Mario Del Rosario @mariodelr.bsky.social tell us all about PhototFiTT, the framework they developed for measuring phototoxicity in live-cell fluorescence imaging.
focalplane.biologists.com/2026/03/02/i...