📄🔬 Congratulations to all authors on this excellent preprint showcasing #NanoFLex, a FLIM strategy that enables lifetime-based separation of multiple targets in a OneStep-IF using our #SmartSecondaries fused to different #HaloTag variants. 👏

We’re proud that SmartSecondaries® power this workflow☺️

New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

📣 Expanding our Halo portfolio!
Introducing Halo Selector - #sdAb -based affinity resin for highly specific, easy & reliable #HaloTag fusion protein #pulldown.

✅ High affinty & capacity
✅ Ultra- low background
✅ Broad buffer compatibility
✅ Works with bound ligands

Learn more: bit.ly/465BKnm

New! abberior CELL LINE – Mito-SNAP / ER-Halo
Visualize mitochondria & ER in real time with SNAP- & Halo-tags. Stable, easy to culture, and perfect for live-cell imaging and probe testing.

Learn more: sales@abberior.com

#LiveCellImaging #SNAPtag #HaloTag #abberior

What enables reproducible, long-term in vivo imaging?
Our latest blog post explores how lyophilized HaloTag® ligands with Janelia Fluor® dyes are changing neuroscience imaging. #InVivoImaging #Neuroscience #ProteinLabeling #FluorescenceImaging #HaloTag www.promegaconnections.com/mapping-the-...

FluoTag®-X4 anti-Halo (N5004) sdAb-based reagent for HaloTag detection via direct IF_NanoTag Biotechnologies

📣 HaloTag fans, meet 𝐅𝐥𝐮𝐨𝐓𝐚𝐠®-𝐗𝟒 𝐚𝐧𝐭𝐢-𝐇𝐚𝐥𝐨 – our new #sdAb -based fluorescent reagent for direct, bright & highly specific labeling of #HaloTag fusion proteins.

• 2 sdAb clones = amplified signal
• preserves HaloTag ligand binding

Learn more: bit.ly/3ULE5OK

#Nanobody #ProteinLabeling #Imaging

Great work by @hanasedlackova.bsky.social & team expanding on applications of the #HaloTag. Congrats!

Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes HaloTag (HT) is a versatile self-labeling protein that has been widely adopted in fluorescence microscopy. Besides its established use as an intensity-based marker and sensor, interest in using HT fo....

Happy to share the results of a fun side project in which we used the COUPY dye and mutants of HaloTag7 to show that changes in fluorescence lifetime correlate with the polarity of the protein binding pocket!
chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1...
#FLIM #fluorescence #HaloTag

Thanks to @abberior.rocks for having me and the exchangeable #HaloTag Ligands! The workshop and #TiM2025 were a blast 😊