mtHsp70 chaperone converts mitochondrial proteostasis stress into impaired protein import | PNAS www.pnas.org/doi/full/10....

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation www.pnas.org/doi/10.1073/...

Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover - Nature Communications Proteasomes typically degrades ubiquitin-tagged proteins, but midnolin enables a distinct ubiquitin-independent pathway. Here, the authors present cryo-EM structures of the midnolin-proteasome complex...

Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover www.nature.com/articles/s41...

NorQD AAA+ complex drives metal insertion by a twisting mechanism - Nature Communications AAA+ -ATPases drive diverse molecular remodeling processes in cells. Here, the authors characterize the interaction between the NorQ ATPase and its VWA domain partner NorD, and propose a twisting mech...

NorQD AAA+ complex drives metal insertion by a twisting mechanism www.nature.com/articles/s41...

Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover pubmed.ncbi.nlm.nih.gov/41896529/ #cryoEM

Check out the final version of this work, out today! @natcomms.nature.com www.nature.com/articles/s41...

Registration now open! 2026 #EMBO #Ubiquitin Workshop set in stunning Monopoli, Puglia 🇮🇹
Cutting edge science, great speakers, short talk opportunities, travel awards
Limited spaces. Dont miss out - register early!
Registration & more info: meetings.embo.org/event/26-ubi...

Structure-function analysis of the bacterial ClpE/ClpP AAA+ protease General and regulatory proteolysis in bacteria is executed by a set of ATP-dependent proteases composed of hexameric ring-forming AAA+ proteins and associated peptidase barrels (e.g. ClpP). These AAA+...

Structure-function analysis of the bacterial ClpE/ClpP AAA+ protease - Journal of Biological Chemistry www.jbc.org/article/S002...

Surprisingly: "Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana".
The shining bounds of N-degron pathway influence expands! @charlene-kunaka.bsky.social www.biorxiv.org/content/10.6...

Join us in Palermo!
FEBS Workshop Protein Termini 2026: the power of protein termini across bacteria, plants, and animals—from ribosome biology to proteostasis and applications. Deadline: 3 March 2026
proteintermini.org/meeting
#ProteinTermini #Proteostasis #FEBS #EMBO @iubmb.bsky.social

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳

mrc.tal.net/vx/mobile-0/...

A dynamic displacement mechanism drives protein import into mitochondria Most mitochondrial proteins are produced in the cytosol and imported through the translocase of the outer mitochondrial membrane (TOM) to reach their final destination. Although this protein entry gat...

📢 New preprint alert!
How do proteins enter mitochondria? We uncovered a surprising mechanism at the mitochondrial entry gate—using #NMR, in vivo single-particle tracking, yeast experiments, and MD simulations to crack the code.
www.biorxiv.org/content/10.6...
#StructuralBiology #Mitochondria
🧵 1/8

The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis Large oligomeric proteins constitute a major fraction of proteomes, but are difficult to refold in vitro, raising the question of how cells direct their biogenesis. Roeselová and Shivakumaraswamy et a...

Our latest cotranslational folding story is now published @cp-molcell.bsky.social. Really cool (I think) new ideas about how exactly the ribosome directs folding and assembly to make sure complicated proteins mature efficiently in cells.
www.cell.com/molecular-ce...

Single-molecule dynamics of the TRiC chaperonin system in vivo - Nature Single-particle tracking experiments in intact cells reveal dynamic co- and post-translational interactions of the TRiC–PFD chaperonin complex with client proteins during in vivo protein folding.

I’m excited to share my first-author paper, with co-first author @rongqinxiaoxiao.bsky.social, now out in @nature.com. We developed a live-cell single-particle tracking platform to see how TRiC & prefoldin engage proteins during co- and post-translational folding. 1/9 www.nature.com/articles/s41...

Molecular mechanisms of mitochondrial AAA+ proteases Mitochondrial AAA+ proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association ...

Molecular mechanisms of mitochondrial AAA+ proteases - Journal of Biological Chemistry www.jbc.org/article/S002...

Recognition of small Tim chaperones by the mitochondrial Yme1 protease Yme1 is a conserved ATP-dependent protease that maintains mitochondrial function by degrading proteins in the intermembrane space. However, how Yme1 selects substrates within the crowded mitochondria...

Recognition of small Tim chaperones by the mitochondrial Yme1 protease onlinelibrary.wiley.com/doi/10.1002/...

The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis Natural proteins often form intricate multidomain, oligomeric architectures. This presents a prima facie challenge to cellular homeostasis, as topolog…

The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis www.sciencedirect.com/science/arti...

A stochastic mechanism drives fast substrate translocation in the AAA+ machine ClpB www.nature.com/articles/s41...

And another paper with contribution from us. Here Sascha Amann solved the structure of the BORC complex. Due to its shape it is extremely challenging for cryo EM analysis but still he cracked it. Read the full story spearheaded by the Huber lab in Innsbruck: www.pnas.org/doi/10.1073/...

The US is quitting 66 global agencies: what does it mean for science? The United States is leaving some of the world’s oldest and most influential scientific networks involved in biodiversity research, climate science and conservation. Affected organizations tell Nature that their work continues.

The US is leaving some of the world’s oldest and most influential scientific networks involved in biodiversity research, climate science and conservation

go.nature.com/4a48Ney

@support.bsky.team

[476084-quiet-morning-calm].

A phage protein screen identifies triggers of the bacterial innate immune system - Nature Microbiology A library of 400 phage protein-coding genes is used to find a trove of antiphage systems, revealing systems that target tail fibre and major capsid proteins.

I’m thrilled to share our work on phage triggers of the bacterial immune system in its final form @natmicrobiol.nature.com www.nature.com/articles/s41...

portlandpress.com/biochemsoctr...

Using complexes obtained through PINK1 pulldown, researchers determine the cryo-EM structures of the human Hsp90-Cdc37-PINK1 complex at 2.84 Å, Hsp90-FKBP51-PINK1 at approximately 6 Å, & Hsp90- PINK1 at 2.98 Å
www.nature.com/articles/s41...

It’s a national disgrace and unfortunately just a confirmation that Australia (and this government) does not value scientific research

Save the CSIRO

Our national science & research agency is now facing more job cuts than under Tony Abbott

Call on the Albanese Govt to fund the scientists & research we need to tackle big issues we face from climate to food security and everything in between

www.davidpocock.com.au/save_our_csiro

Add this to the CSIRO cuts and uni cuts generally and the picture is unmistakable

We are all mosaics: vast genetic diversity found between cells in a single person @nature.com
www.nature.com/articles/d41...
@biorxivpreprint.bsky.social ⁦@mayoclinic.org @harvardmed.bsky.social @timcoorens.bsky.social @emblebi.bsky.social
www.biorxiv.org/content/10.1...

A triple-action PROTAC for wild-type p53 cancer therapy @cp-cellrepmed.bsky.social @lorenwalensky.bsky.social @danafarber.bsky.social
www.cell.com/cell-reports...