Peptide-centric local stability assay (PELSA) for sensitive identification of ligand-targeting proteins and binding sites at proteome scale Nature Protocols, Published online: 22 April 2026; doi:10.1038/s41596-026-01354-wThe peptide-centric local stability assay (PELSA) is used to identify ligand targets and binding regions at the proteome scale. By subjecting native proteins to brief yet extensive trypsin digestion, this method generates local stability profiles.

New Article! Peptide-centric local stability assay (PELSA) for sensitive identification of ligand-targeting proteins and binding sites at proteome scale

Professor Claire Vallance (Oxford Chemistry) will be speaking in London at this year’s Pint of Science festival on 19 May.

An accessible look at the chemistry of interstellar space and the molecules shaping our universe.

1 month left!

www.brixenproteomics.org

Thinking about getting a windows on arm laptop...but then I remember I have to use MS software some of which still plays with MSDOS 3.1 rules. Sigh.

Meet this year’s Plenary Speakers who are shaping the conversations at #ASMS2026!

Promotional graphic with text: SPECIAL ISSUE | Journal of the American Society for Mass Spectrometry logo | 2024 Fenn: Photoactivation and Ion Activation | Read More | ACS Publications logo

This Special Issue was created in honor of Professor Jenny Brodbelt, recipient of the 2024 ASMS John B. Fenn Award for a Distinguished Contribution in Mass Spectrometry.

Read the Special Issue and editorial written by JASMS Associate Editor Lingjun Li: go.acs.org/e4o

I can measure my growth, and specifically my maturity, by how I handle these type of rejections over the years.

Need to buy a new processing pc for DIA data and i think i cannot, ethically, do it. Those RAM prices are insane! Grrrrr!

screenshot of the first few pages of my first paper (novel ms instrument) and a very recent paper (novel ms instrument)

Someone asked me yesterday if my science has evolved over the last twenty years... i present my first paper and a very recent paper. looks like a no! lol

Deep visual proteomics uncovers nociceptor diversity and pain targets - Nature Communications Sensory neuron subtypes are defined by transcriptomics, but their proteomic identities remain unclear. Here, authors show distinct protein signatures of electrophysiologically defined nociceptors and ...

Excited to share our new @natcomms.nature.com study with the @garyrlewin.bsky.social lab and @amapruns.bsky.social. Using deep visual proteomics, we created the first neuronal subset-resolved proteomic map of sensory neurons with potential implications for pain treatment.
go.nature.com/481nr4R

Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection Does HIV-1 package host proteins that promote infection? Using in virion RNA interactome capture, Garcia-Moreno et al. mapped human proteins bound to the viral genome inside virions. They identified o...

We used our new ivRIC method to map host proteins inside HIV-1 particles, finding 100+ proteins that the virus hijacks to boost infection.

This project has a long history, spanning from pre-COVID to now! Driven by Manuel Garcia-Moreno and led by @alf-castello.bsky.social

tinyurl.com/54vb842w

🧵 1/8 Why do we invest billions in drugs, BUT far less in the diagnostics needed to ensure they’re used effectively? That question motivated our new @science.org paper on diagnostics, innovation, and disease burden.

Out now at PLoS Pathogens @plos.org. Nice collaboration with @danielhurdiss.bsky.social @utrechtvirology.bsky.social. Some beautiful glycoproteomics work by @tshamorkina.bsky.social mapping N- and O-glycan shield of coronaviruses infecting whales and dolphins 🐋🐬🐳.
journals.plos.org/plospathogen...

Moving office and found a banger!

Project Hail Mary is very good if you ignore the centrifuge debacle. Worthy successor to The Martian.

Vibronic and Spin–Orbit Interplay Governs Ultrafast Spin Crossover in an Iron(II) Carbene Complex Spin-vibronic quantum dynamics based on TDDFT/TDA parameterization and hierarchical equations of motion (HEOM) simulations reveal ultrafast intersystem crossing in [Fe(bmip)2]2+. Population transfers...

Our work on ultrafast spin crossover in iron(II) carbene complexes is now published. Using ab initio methods and HEOM simulations, we explore how vibronic and spin-orbit interactions govern excited-state dynamics and lifetimes.

chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

Source: ZME Science
share.google/2PPRYYY54Lhk...

RNA binding as an emerging regulatory dimension in the type I IFN response Iselin et al. explore how the type I IFN response regulates RNA-binding activity using RNA-interactome capture and extend the RIC protocol to assess the role of phosphorylation in this process. Focusi...

Proud of Louisa Iselin. Not the easiest PhD, covid, two supervisors (shabazlab.bsky.social) moving labs, but she pushed through and delivered a really solid piece of work on how interferon reshapes RNA-protein interactions post-translationally www.cell.com/cell-reports...

RNA binding as an emerging regulatory dimension in the type I IFN response Iselin et al. explore how the type I IFN response regulates RNA-binding activity using RNA-interactome capture and extend the RIC protocol to assess the role of phosphorylation in this process. Focusi...

Proud of Louisa Iselin for this work. Not only a covid baby but had both of her supervisors move lab (@alf-castello.bsky.social and me). The works is a meticulous analysis of the interferon pathway and specifially how RNA binding of proteins are modulated by PTMs.

www.cell.com/cell-reports...

Oxford during the golden hour is something rather special. Also, why does it feel a bit weird to act like a tourist in your workplace?!

Congratulations to John R. Yates III, Ruedi Aebersold and Matthias Mann, recipients of the 2026 Canada Gairdner International Award.

Prof. Yates: www.gairdner.org/winner/john-...
Prof. Aebersold: www.gairdner.org/winner/ruedi...
Prof. Mann: www.gairdner.org/winner/matth...

#2026GairdnerAwards

Host cell remodeling via cyclin dependent kinases drives Ebola virus replication and transcription.

www.biorxiv.org/content/10.6...

Check out our latest preprint.

Great work by @tshamorkina.bsky.social. Thanks to co-authors Danni Snikkers and @hecklab.bsky.social

Structure of SARS-CoV-2 spike in complex with its co-receptor the neuronal cell adhesion protein contactin1

www.biorxiv.org/content/10.6...

Check out our new preprint. Cool structure by Sabrina Krepel with Bert Janssen.
Thanks to @danielhurdiss.bsky.social @utrechtvirology.bsky.social #covid #pais

JASMS welcomes Dr. Yury Tsybin as Associate Editor — the first from the industrial sector. His appointment strengthens the connection between the journal and the many industry scientists advancing mass spectrometry.

Learn more: https://bit.ly/4s6dMlG

#ASMS #MassSpecCommunity

Unpaired data as a first-order challenge in single-cell and spatial proteomics - @mkoutrouli.bsky.social @panosroussos.bsky.social @scverse.bsky.social go.nature.com/3NHDkGP

matrixscience.com is being overwhelmed by millions of HTTP requests from distributed botnets, essentially a distributed denial of service (DDoS) attack. We are forced to mitigate it by restrict access to certain pages of dynamic content.

www.matrixscience.com/whats_new/mi...

#proteomics #massspec

Post Docs are junior doctors/resident doctors of the scientific world. Current funding cuts combined with their traditionally poor treatment is turning off a generation of PhD students. Long term effects will be devastating. This 'correction' will be effectively permanent.

Investigating the Efficiency of Ultraviolet Photodissociation in Peptides Modified with N-Terminal UV-Absorbing Chromophores Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry-based proteomics. However, the UVPD efficiency depends on the presence of UV-absorbing chromophores within analytes. Here, we systematically investigate how N-terminal modification of peptides with aromatic chromophores influences the 193 nm UVPD efficiency and fragmentation behavior. Using NHS ester chemistry, human cell lysate digests were derivatized with a range of UV-absorbing aromatic labels and analyzed on an Omnitrap–Orbitrap platform equipped with a 193 nm ArF excimer laser. Compared with unmodified controls, derivatized peptides displayed a modestly increased fragmentation efficiency and a greater diversity of sequence-informative fragments, particularly b-type ions. Peptides lacking aromatic amino acids benefited most from N-terminal aromatic labeling, exhibiting enhanced fragmentation and sequence coverage. These findings demonstrate that incorporation of aromatic chromophores at the N-terminus can enhance UVPD performance and improve spectral interpretability, thereby expanding the applicability of UVPD in bottom-up proteomics workflows.

Our humble contribution to Journal of the American Society for Mass Spectrometry special issue “Fenn: Photoactivation and Ion Activation”.

pubs.acs.org/doi/10.1021/...

Meet Bini Ramachandran, featured in this month’s Faces of Mass Spectrometry. Her journey highlights the diverse paths and perspectives shaping the mass spectrometry community.

Read her story: https://bit.ly/3OZJrqa

#ASMS #FacesofMassSpec #FEMS

Zero-Dalton Resolution in Nanopore Peptide Recognition Achieving zero-dalton resolution─the discrimination of biomolecules with identical mass─remains a fundamental challenge in molecular analysis. Nanopore sensing can resolve short peptide isomers, but w...

Zero-Dalton Resolution in Nanopore Peptide Recognition | Analytical Chemistry pubs.acs.org/doi/10.1021/...

"Zero Dalton resolution" is a terrible name