My former lab at the University of Oxford is looking for a postdoc to study ADP-ribosylation and ubiquitination signalling in DNA damage using biochemistry and structural biology.

my.corehr.com/pls/uoxrecru...

Informal inquiries can be sent to Prof Ivan Ahel via email: ivan.ahel@path.ox.ac.uk.

Cat burglars, yeast races, and other hypothesis-driven bioscience practicals, edited by Dr Rebecca Barnes We are pleased to announce the publication of a new Open Educational Resource (OER) on the Sheffield Pressbooks Network.

After a very "dry" January and a long day in a curriculum development workshop, it was nice to go to the pub last night and celebrate our OER book launch with the librarians who helped us publish.

doi.org/10.15131/she...

sheffield.pressbooks.pub/hypothesisba...

www.sheffield.ac.uk/library/news...

I think one has been created recently

bsky.app/profile/open...

Check out this short book I've co-authored. It's aimed at University staff seeking lab practicals for biosciences students. It contains eight projects with a molecular focus, student-facing materials, and resources for teachers.

sheffield.pressbooks.pub/hypothesisba...

doi.org/10.15131/she...

Crystal Structure of an Intracellular Subtilisin Reveals Novel Structural Features Unique to this Subtilisin Family The intracellular subtilisin proteases (ISPs) are the only known members of the important and ubiquitous subtilisin family that function exclusively within the cell, constituting a major component of the degradome in many Gram-positive bacteria. The first ISP structure reported herein at a spacing of 1.56 Å reveals features unique among subtilisins that has enabled potential functional and physiological roles to be assigned to sequence elements exclusive to the ISPs. Unlike all other subtilisins, ISP from B.

Shameless self promotion 😊

Crystal Structure of an Intracellular Subtilisin Reveals Novel Structural Features Unique to this Subtilisin Family

www.cell.com/structure/fu...

Snow-covered back garden

Happy New Year from snowy Sheffield! 🍾🥂
I hope you enjoyed the festivities and got some nice pressies (I asked Santa for a winning lottery ticket, but alas, he did not oblige ... I guess I must have been a naughty boy 😨).

Deadline today, January 6th! Two PhD studentships with us - fully funded and open to students worldwide. Come help us learn more about our favourite #Cdiff #phage

Congratulations Elena! Fantastic way to start the New Year. 🍾🥂

There is ONE WEEK left to apply for the #PlantScience #PhD position in my lab! This will use the latest #microscopy, #biosensor and #ecophysiology techniques!

Happy New Year, Karla! 🍾🥂

Zinc-Dependent Proteins Could Hold Key to Cancer and Viral Therapies The molecular mechanism has been uncovered by which zinc-dependent proteins, known as macrodomains, reverse ADP-ribosylation—a key regulatory process in cells linked to cancer and viral infections.

Interested in ADP-ribosylation? Technology Networks has published a short news article I wrote to describe the results and implications from my latest published research.
www.technologynetworks.com/tn/articles/...

doi.org/10.1016/j.jb...

Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield on FindAPhD.com PhD Project - Unveiling the Secrets of Intrinsically Disordered Proteins in Gene Regulation at University of Sheffield, listed on FindAPhD.com

We have a #PhD available! I'm really excited about this project, looking at whether and how differences in the #IDRs of paralogous epigenetic enzymes CBP and p300 cause them to behave differently. www.findaphd.com/phds/project...

1. I feel like this is such obvious advice that it gets taken for granted & consequently no one says this to young scientists but READ PAPERS, read all the papers. As you move up in your career you will have less & less time to do this. Read everything that appeals to you not just in your field

Unlocking the secrets of metastasis: Cancer cells' 'eating' ability could lead to new treatments Cancer is one of the most formidable health challenges of our time, affecting nearly half of us during our lives. While treatments have significantly improved in recent decades, the biggest challenge ...

Unlocking the secrets of metastasis: Cancer cells' 'eating' ability could lead to new treatments medicalxpress.com/news/2024-12... via
#medical_xpress

Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi As part of a drug discovery programme to discover new treatments for human African trypanosomiasis, recombinant trypanothione reductase from Trypanoso…

Shameless self promotion 😊

Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi

www.sciencedirect.com/science/arti...

Specificity of the trypanothione‐dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzyme Trypanothione replaces glutathione in defence against cellular damage caused by oxidants, xenobiotics and methylglyoxal in the trypanosomatid parasites, which cause trypanosomiasis and leishmaniasis....

Shameless attempt of self-promotion 😊

Specificity of the trypanothione‐dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzyme

onlinelibrary.wiley.com/doi/10.1111/...

Excited to see our paper in press today at HGG Advances!!! We described a new variant in #ADPRS gene in individuals with CONDSIAS, disrupting its #ARH3 protein function leading to increased #ADP-ribosylation in cells.

bit.ly/3OmvwqH

a, The mechanism of ADP-ribosylation for Arg. Initially, the N-glycosidic bond between the ribose and nicotinamide is destabilized by a Glu residue of an ART. This leads to the formation of an oxocarbenium ion of ADPr, with nicotinamide as the leaving group. This electrophilic ion is attacked by a nucleophilic Arg residue of the acceptor protein after Glu-mediated proton abstraction, leading to the formation of an N-glycosidic bond50. b, Our proposed RNAylation-reaction mechanism. In a similar way to ADP-ribosylation in the presence of NAD, we propose that ARTs might use NAD–RNA to catalyse an RNAylation reaction, thereby covalently attaching an RNA to an acceptor protein. Red, nicotinamide riboside of NAD and NAD-RNA; blue, catalytic residues of the ART; purple, nucleophilic Arg residue of the acceptor protein.

A viral ADP-ribosyltransferase attaches RNA chains to host proteins (2023).
www.nature.com/articles/s41...

ADP-ribosylation from molecular mechanisms to therapeutic implications

Asymmetric nucleosome PARylation at DNA breaks mediates directional nucleosome sliding by ALC1 - Nat... Bacic et al. demonstrate that PARP1/HPF1 preferentially modify histone tails closest to the DNA break, directing ALC1-catalyzed nucleosome sliding. These findings suggest a mechanism for rendering DNA...

New paper published! 😊
Nucleosomes, ADP-ribosylation, a chromatin remodeler, smFRET, cryoEM. And the best of all: it confirmed a hypothesis we posited in our previous paper in 2021! Super nice to finally see this new paper out. 🤩 🧬🧶
doi.org/10.1038/s414...

Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I

Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I

onlinelibrary.wiley.com/iucr/doi/10....

Ignore this, it's a test to see if metric sites can pick up this mention or not (my earlier post seems to have been ignored by them)

Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA (#RAD51 homologue)

academic.oup.com/nar/article/...

#OpenAccess Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA (#RAD51 homologue): t.co/fpbNDxUTeS