#microsky #phagesky on #phage cocktails

Parasitic connections: a patescibacterial epibiont, its methylotrophic gammaproteobacterial host, and their phages
#microbiology #bacteria #phage #virus #MicroSky
@asm.org
doi.org/10.1128/mbio...

Another week, another paper that we liked so much, we made a brief comment on

You should take a look! It's only a short thread 🧵

#Phage #Phagesky

www.pnas.org/doi/10.1073/...

Phages drive the dissemination of antibiotic resistance genes by facilitating host adaptation to heavy metal stress

#phage #phagesky

While more experimental evidence is warranted, this work motivated a vivid discussion in our team!
It also reminds us that we are still far from understanding #phage biology even after >100 years of research.

We're partnering with Parallel Health, a company that is redefining what precision #phagetherapy looks like with their MD-03 Protocol™ - a comprehensive system that integrates #microbiome sequencing, personalized #phage serums, and clinical support. www.bacteriophage.news/parallel-hea...

Living with #Mycobacterium abscessus (Mab)? You could help advance personalized
#phage therapy research, while potentially benefiting from future treatment options.
We're building a patient-focused Mab #biobank. Learn more: tolka.ai/biobank

Cross-order detection of bacteriophage transduction in microbial communities using RNA barcoding - Nature Communications Bacteriophages are the most abundant life form on earth and can be applied to eliminate or engineer bacteria. Here, authors demonstrate RNA barcoding as a high throughput tool to measure bacteriophage...

#microsky #phagesky #phage

www.nature.com/articles/s41...

Phage-Antibiotic Therapy Clears Spine Implant-Associated MRSA | The Phage A successful case report was published in the International Journal of Infectious Diseases, supporting personalized phage therapy treating chronic MRSA vertebral osteomyelitis

In a recently published peer-reviewed case report in the International Journal of #Infectious Diseases, a critical case of chronic MRSA Vertebral osteomyelitis involving spinal hardware was resolved using personalised #phage therapy.

#antibiotics #MRSA

www.thephage.xyz/news/phage-a...

USDA-ARS Vaccine and Phage Research for Aquatic Health - The phage jobs USDA-ARS offers postdoctoral research opportunity in phage therapy, aquaculture disease prevention, vaccine development, and microbiology research.

The U.S. Department of Agriculture (USDA), through its Agricultural Research Service (ARS), is offering a Postdoctoral Research Opportunity at the Stuttgart National Aquatics Research Centre in Stuttgart, Arkansas, USA.

#phage #jobs #career

thephage.xyz/jobs/jobs/us...

Phage therapy in oncology: opportunities for cancer prevention and treatment Bacteriophages (phages) are emerging as programmable biological therapeutics in oncology, extending beyond their traditional antimicrobial applications. This review proposes a phage–microbiome–immune–...

Excited to share this review🔔, we spent sometime thinking about
phage–microbiome–immune–oncology axis for phage therapy in cancer!
#phage #phagesky
Phage therapy in oncology: opportunities for cancer prevention and treatment: Trends in Molecular Medicine www.cell.com/trends/molec...

Structural modeling reveals phage proteins that manipulate bacterial immune signaling The genomes of phages—viruses that infect bacteria—are largely composed of "dark matter": genes that encode proteins whose functions remain unknown. Less than four years ago, a team led by Prof.…

Structural #modeling reveals #phage #proteins that manipulate #bacterial #immune signaling ...

| #bacteria | #MicroSky | #genomes | #virus | #immunesystems | Via @sciencex.bsky.social

We recently had a nice lab discussion about this work and wanted to share some of it on a short thread here 🧵

BTW, word on the street is that prophages are pretty cool 😎

#Phage #Phagesky

www.nature.com/articles/s41...

Discovery of human gut #phage -encoded anti-CRISPR proteins unveils diverse mechanisms for phages to evade type II #CRISPR immunity.

Gut phages encode diverse Acrs; 651 candidates found, including some with dual regulatory & CRISPR‑inhibitory roles.

www.sciencedirect.com/science/arti... #MicroSky

Phage spiders / spore storm II Midjourney prompts by yours truly
#midjourney #giant #monster #creature #phage #spiders #spore

Capturing dynamic phage–pathogen coevolution by clinical surveillance - Nature The acquisition of a parasitic anti-phage mobile genetic element, PLE11, showing potent anti-phage activity against cocirculating ICP1, and the subsequent evolution of ICP1 to escape this defense, are...

#microsky #phagesky #phage

www.nature.com/articles/s41...

Spaceflight supercharges viruses’ ability to infect bacteria Viruses develop tricks to attack bacteria without the help of gravity

#Spaceflight #supercharges #viruses’ ability to infect #bacteria ...

| #Space | #microbes | #gravity | #ISS | #bacteriophages | #Phage | Via @sciam.bsky.social

As the antimicrobial resistance on the rise, scientists are shaking up a new kind of mix, #phage cocktails! Kaneko et al. discuss the latest technologies and strategies for designing effective phage cocktails.
#FEMSMicrobiolRev
buff.ly/on2VDAb

Congrats to Caroline on a VERY nice thesis work!!!!

#phage #phagesky

#microsky #phagesky #phage

Cross-family and phage-specific gene requirements for Klebsiella infection revealed by scalable RB-TnSeq genetic screens Bacteriophages (phages) are being cataloged at unprecedented rates and are recognized as key players in nutrient and energy cycling across ecosystems. Yet, the biological determinants of phage-host specificity and infection success remain poorly understood. Here, we used a randomly barcoded, genome-wide, loss-of-function transposon mutant library (RB-TnSeq) of Klebsiella sp. M5al, a plant-associated, nitrogen-fixing rhizobacterium, to identify bacterial genetic determinants necessary for the infection of 25 double-stranded DNA phages spanning five viral families. This approach identified 42 bacterial genes associated with phage infection, encompassing genes involved in receptor biosynthesis, regulatory pathways, electron transport, and unknown functions. Disruption of genes involved in receptor biosynthesis, such as glycosyltransferases involved in lipopolysaccharide (LPS) production, conferred cross-resistance to half of the phages, while intracellular gene disruptions had phage-specific effects. Taxonomically, we found significant differences in RB-TnSeq profiles across phage families and genera. While bacterial gene requirements are generally clustered by phage genus, we also observed notable divergences within genera. These differences were associated with variation in receptor usage, likely driven by divergence in tail fiber or other host recognition proteins. In some cases, differential reliance on bacterial intracellular factors suggested that even closely related phages may depend on distinct infection strategies, potentially involving phage-specific genes of unknown function. Our findings reveal key genetic dependencies shaping phage-host interactions and offer a framework for predicting infection outcomes in natural and applied settings. ### Competing Interest Statement The authors have declared no competing interest. Department of Energy and Environment, https://ror.org/05d5hbz44, #DE-SC0020173, #DE-SC0023307

www.biorxiv.org/content/10.6...

#phage #phagesky

www.biorxiv.org/content/10.6...

#phage #phagesky

Cross-family and phage-specific gene requirements for Klebsiella infection revealed by scalable RB-TnSeq genetic screens Bacteriophages (phages) are being cataloged at unprecedented rates and are recognized as key players in nutrient and energy cycling across ecosystems. Yet, the biological determinants of phage-host specificity and infection success remain poorly understood. Here, we used a randomly barcoded, genome-wide, loss-of-function transposon mutant library (RB-TnSeq) of Klebsiella sp. M5al, a plant-associated, nitrogen-fixing rhizobacterium, to identify bacterial genetic determinants necessary for the infection of 25 double-stranded DNA phages spanning five viral families. This approach identified 42 bacterial genes associated with phage infection, encompassing genes involved in receptor biosynthesis, regulatory pathways, electron transport, and unknown functions. Disruption of genes involved in receptor biosynthesis, such as glycosyltransferases involved in lipopolysaccharide (LPS) production, conferred cross-resistance to half of the phages, while intracellular gene disruptions had phage-specific effects. Taxonomically, we found significant differences in RB-TnSeq profiles across phage families and genera. While bacterial gene requirements are generally clustered by phage genus, we also observed notable divergences within genera. These differences were associated with variation in receptor usage, likely driven by divergence in tail fiber or other host recognition proteins. In some cases, differential reliance on bacterial intracellular factors suggested that even closely related phages may depend on distinct infection strategies, potentially involving phage-specific genes of unknown function. Our findings reveal key genetic dependencies shaping phage-host interactions and offer a framework for predicting infection outcomes in natural and applied settings. ### Competing Interest Statement The authors have declared no competing interest. Department of Energy and Environment, https://ror.org/05d5hbz44, #DE-SC0020173, #DE-SC0023307

#microsky #phagesky #phage

www.biorxiv.org/content/10.6...

Citrobacter rodentium-infected mouse colon at 8 days postinfection. Mucosal attachment remains unaffected by Eifel2 phage treatment, as visualized via immunofluorescence staining of C. rodentium (green) counterstained with wheat germ agglutinin (purple) and DAPI (blue).

Check out the mBio cover image (Vol 17, Issue 3 - buff.ly/Pqe1IoA) related to our joint publication with the Dept of Life Sciences at @imperialcollegeldn.bsky.social

📰 buff.ly/bcoN2X8

#phage #microbiome #ucc #phagesky #microsky

@uccmicrobiology.bsky.social @colinhillucc.bsky.social @asm.org

Bacteriophage-Encoded Small RNAs: Emerging Tools for Phage Therapy and Antibacterial Intervention - Aviezer Silverman, Sahar Melamed, 2026 Bacteriophages (phages) are viruses that specifically infect bacteria and play a central role in shaping microbial communities and bacterial evolution. Beyond t...

🚨Happy to share our new mini-review on phage-encoded small RNAs and their potential for phage therapy and antibacterial strategies, written with Aviezer Silverman!
journals.sagepub.com/doi/10.1177/...
#RNA
#phage
@journals.sagepub.com
@hebrewuniversity.bsky.social
@phagedirectory.bsky.social

#phage #phagesky

Even though DMS3 is a #Pseudomonas #phage, Nathan showed that Aqs1 could inhibit the function of other #T4P family ATPases, including #T2SS GspE, PilB from #Stenotrophomonas (twitching) and PilB from #Acinetobacter (DNA uptake). These data point to a new allosteric vulnerability of PilB homologues.

New paper alert! Outstanding work by PhD candidate Nathan Roberge, great collab with the Ellison and Maxwell labs. #Pseudomonas #phage DMS3 encodes Aqs1, an inhibitor of #T4P assembly ATPase PilB, but how it binds and leads to loss of function was unknown. @mcmasteriidr.bsky.social

Phage The Untouchable: La Comandante “Imposible” Que Convierte Cualquier Error En Derrota Instantánea. Phage the Untouchable es una comandante extrema: si no la “desbloqueas” puedes perder al castearla, pero bien construida encadena derrotas instantáneas y sigue siendo una carta cara y única.

¡🦠💀 Phage the Untouchable: la comandante "imposible" que convierte errores en derrotas instantáneas en Commander! Desbloquéala con Command Beacon o Torpor Orb, protégela con Greaves y elimina rivales. ¡All-in mono-negro letal!

#AncestralGames #Phage #MTGÑ

ancestral.games/articulos/ph...

A virus hiding inside bacteria may help explain colorectal cancer The gut bacterium Bacteroides fragilis has long presented researchers with a paradox. It has been associated with colorectal cancer, yet it also lives quite happily in most healthy people. A study by…

#Virus hiding inside #bacteria may help explain #colorectal #cancer ...

| #Bacteroides | #microbiome | #bacteriophages | #phage | Via @sciencex.bsky.social