Phenotype to genotype: A new and rapid approach using whole-genome sequencing Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...

Check it out in #PLOSGenetics: journals.plos.org/plosgenetics...

We've used WheresWalker to map our newly discovered #darkyolk zebrafish mutants which have defects in #ApoB-lipoprotein metabolism. We map zion to slc3a2a, a heterodimeric amino acid transporter, and find that several SLC3a2/SLC7s are associated with ApoB phenotypes in humans.

Validated in #zebrafish & #maize, this pipeline uses a combination of #mapping-by-sequencing and #recombinantmapping to narrow the number of candidate genes which can be tested using #F0crispr

Phenotype to genotype: A new and rapid approach using whole-genome sequencing Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...

Announcing WheresWalker! A fast, accurate mutation-mapping pipeline that accelerates gene discovery with forward genetic screening.

WheresWalker: a pipeline for rapid mutation mapping using whole genome sequencing Forward genetic screening is a powerful approach to assign functions to genes and can be used to elucidate the many genes whose functions remain unknown. Chemical mutagenesis is an unbiased and effici...

Read more about the screen that led to our mutant discoveries, and the mapping tool (#WheresWalker) we're using to map our loci here: www.biorxiv.org/content/10.1...

Using forward genetic screening, we discovered 28 novel zebrafish mutants
@zebrafishrock.bsky.social
and named them after our parks
@altnps.bsky.social
🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.

Last week was #NationalParkWeek! To celebrate - meet our National Park inspired #zebrafish mutants. From acadia to zion, our “dark yolk” mutants are shedding light 💡on new biology that will help us to better understand how diabetes and cardiovascular disease develop.