Figure 1 from the linked preprint: "OrganoidChip+’s dimensions and principle of work. (a) The chip schematic depicting its height dimensions and various compartments such as the inlet, perfusion channel (PC), culture chamber (CC), trapping areas (TAs) consisting of staging chambers (SCs) and immobilization chambers (ICs), filter channels (FCs), exit chamber (EC), and serpentine exit channel. The serpentine exit channel with a length of 43.5 mm and a width of 215 µm generates a hydrodynamic resistance of 3 × 1012 (N.s/m5) to avoid high flow rates and shear stress inside the chip. (b) Various steps for cell seeding (i), organoid culture (ii), and immobilization (iii) in the chip. (i) For seeding, the Luer stub is inserted into the culture chamber, the cell suspension is dispensed with a rotating movement to fill the entire culture chamber. (ii) Organoids grow efficiently while accessing nutrients from all direction surrounding the culture chamber. (iii) Matrigel is digested, and organoids are pushed into the TAs for immobilization while some organoids are natively immobilized by adhering to the glass in the culture chamber. (c) Side view of the same steps depicted in (b). The organoids in the culture chamber are imaged using brightfield microscopy every day to track their growth. After 7 days of culturing, Matrigel is digested to enable organoid immobilization within TAs. After immobilization, organoids can be fluorescently labelled and imaged at high-resolution on the chip. Scale bars represent 1 mm in (a-b) and 400 µm in (c)."
You've heard of #organoids and #organchips, but what about an OrganoidChip+? 🧪
Scientists at @utbiomedical.bsky.social & UGA developed this new, imaging-ready #microfluidic platform for research, drug screening, & disease modeling.
Preprint by Moshksayan et al.
www.biorxiv.org/content/10.1...
