Our findings have important consequences for TRPM3-targeted drug discovery and therapy.

Great collaboration with the Thomas Voets, Joris Vriens, CD3 and CISTIM labs.

Out at Nat Commun: Stereoselectivity and plasticity of a common binding pocket in TRPM3

One pocket, many ligands. CryoEM & electrophysiology reveal a promiscuous site where mutations rewire stereoselectivity and invert the functional outcome of ligand binding

nature.com/articles/s41467-026-71226-0

Game changer, no doubt. Commercial systems will have expensive consumables I suppose, but huge savings to be made in cell culture, and many important but sparse native targets come into play.

Crispr-tagged targets from iPSC neurons, for example.

TRPC6 blind? 🤯

Congratulations to first author Gangadhar Eluru, co-authors Steven De Gieter, Stephan Schenck, Annelore Stroobants, Binesh Shrestha, Paul Erbel and Ruslan Efremov.
#MISO #CryoEM #StructuralBiology #MembraneProteins #VIB-VUB Center for Structural Biology #NatureMethods #DrugDiscovery #BioRender

MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.

Or from a single dish of HEK cell culture in the case of two membrane proteins.

Out in Nature Methods now! lnkd.in/gpyBSceg

Wonderful collaboration with the Efremov lab.

Cryo-EM maps and atomic models of the biotin-containing 3-methylcrotonyl-CoA carboxylase (MCC) complex and long-chain acyl-CoA carboxylase (LCC) complex

New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

Happy to announce our most recent preprint: Stereoselectivity and plasticity of a common binding pocket in TRPM3, by cryo-EM and elecrophysiology. Great collaboration with the Voets, Vriens and CISTIM labs.

www.biorxiv.org/content/10.1...

Happy to share the latest from the lab, led by Daniel Alvarez, in collaboration with @lizconibear.bsky.social‬. In this AA-MD tour-de-force, we delve deep into the mechanism and energetics of lipid uptake by bridge-like lipid transfer proteins, and we learn a few interesting things along the way...

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...

Happy to share the latest work from the lab, led by @mudgal17.bsky.social‬, in collaboration with the Weis lab @ethzurich.bsky.social.
How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)

A covalent recognition strategy enables conspecific mate identification The olfactory system can detect an uncountable number of volatile molecules while retaining the ability to discriminate amongst very similar ones. We identified a unique mechanism employed by insect o...

New preprint in the lab combining what we like best: insects, love, chemistry and the olfactory system! 1/8
www.biorxiv.org/content/10.1...

Excited to share our latest story in Nature. We developed a high-throughput cellular stretch system to look for genes regulating membrane rupture under tension, and found an amazing small protein called NINJ1 that weakens the membrane for rupture! H/T to Zozo, my 10-y.o. for her artist's impression😃

Check out this remarkable debut paper from @jie-xu.bsky.social, a very creative lab alumnus. Very proud!

www.nature.com/articles/s41...

I’m thrilled to finally share this preprint! It contains a WOW Cryo-EM structure (likely one of the strongest protein fibers known to man 🤯), but we’ve also untangled its biological function: a novel type of virulence factor💀!
www.researchsquare.com/article/rs-6...

In our latest preprint we used cryoEM to solve the structure of A-ENA fibers and show that they are stabilized by 10 isopeptide bonds per monomer. A-ENA couples the spore to the cry-toxins, and in doing so increases the virulence of Bacillus thuringiensis: www.researchsquare.com/article/rs-6...

Congrats to first author Stephan Schenck, and collaborators Toon Laeremans and @jansteyaert.bsky.social

Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams - Nature Communications SV2A, an essential membrane protein in neurotransmission, is known as the receptor of clostridial neurotoxins and target of anti-epileptic drugs. Here the authors show details of the binding site for ...

I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social
 
www.nature.com/articles/s41...

Please RT. Post-doc opportunity alert! 💥 Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process.

www.nature.com/articles/s41...
www.nature.com/articles/d41...

Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com

Structural basis of ClC-3 inhibition by TMEM9 and PI(3,5)P ₂ pubmed.ncbi.nlm.nih.gov/40093093/ #cryoEM

Mutant tau filaments from some inherited frontotemporal dementias show the Alzheimer fold Nature Structural & Molecular Biology - Frontotemporal dementias can be caused by mutations in MAPT, which encodes the protein tau; the mutant protein forms harmful aggregates in the brain....

Happy to share our latest publication on
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social

Themis and Grb2 form a constitutive structural hub in TCR signaling
doi.org/10.1101/2025...
@savvideslab.bsky.social

Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...

Cryo-EM structures of mammalian membrane proteins from a single cell culture dish?

Yes, Micro ISOlation (MISO) sample preparation makes it possible.

www.biorxiv.org/content/10.1...

Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.

Love this.. 👏

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony

www.biorxiv.org/content/10.1...