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authors.elsevier.com/c/1mv8615Dr~...

Modulation of glycosylation in the brain: A single-cell exploration of glycosidases using the Allen Brain Cell Atlas The mammalian brain is enveloped in a dense, charge-rich glycocalyx that orchestrates cell signalling, migration, and synaptic stability. Transcriptom…

As part of a special issue on "Glycosyltransferases", we are pleased that the editors allowed our glycosidase focused mini perspective data exploration article of the Allen Brain Cell Atlas to be part of the collection 😁

#glycotime @alleninstitute.org

www.sciencedirect.com/science/arti...

It also reminds people that for gene enrichment type analysis, if glycosylation related pathways dont seem significant because the number of genes/proteins changing are not high, be aware that effect and impact of 1 glycoenzyme can be broad

Reduction of Charged Glycoconjugates in Tango2 KO Mouse Brain The transport and Golgi organization 2 (TANGO2) gene has been identified to cause a rare recessive genetic disorder known as TANGO2-deficiency disorder (TDD). TDD is a multi-systemic disorder that ca....

Sharing our new paper where we did our sssmug protocol, found differences in GAGs and sialylation, and it matched exactly the only 2 differentially expressed glycoenzymes.

#glycotime

onlinelibrary.wiley.com/doi/10.1002/...

An AI generated image showing a dense forest of glycans on the surface of the cell.

Have you ever wondered how many N-Glycans or #glycoproteins there are on cells?

The numbers are massive: calculations suggest about 20 to 200 million N-glycans per human cell!

Read the full blog post: research.bidmc.org/ncfg/blog/24...

#glycobiology #glycotime #cells #biology

This is a fantastic day for Australian chemistry. First Australian chemistry Nobel prize since John Cornforth in 1975, but Richard Robson's work is at least as impactful. #ozchem

a cartoon drawing of a pitcher and cup of sugar on a table ALT: a cartoon drawing of a pitcher and cup of sugar on a table

Super excited by our new #glycotime preprint! A long-term project from my group with awesome collaborator @elisafadda.bsky.social

Regulation of N-glycosylation efficiency by eukaryotic oligosaccharyltransferase www.biorxiv.org/content/10.1...

thread below 👇

How easy is it to fudge your scientific rank? Meet Larry, the world’s most cited cat “Exercise in absurdity” reveals flaws in Google Scholar’s productivity metrics

How easy is it to fudge your scientific rank? Meet Larry, the world’s most cited cat www.science.org/content/arti...

"More than 130 citations in 4 years." Meow, Larry!

Oh wait. “All you have to do is put some fake papers on ResearchGate.” And buy citations.

Critical: Let's clean up research!

Photo courtesy from Katherine

Australian Proteomics Analysis Facility (APAF) celebrating 30 year anniversary. Had a good morning on the past, present, and future of proteomics by the pioneers in the field, including Prof. Nicki Packer @sugar-princess.bsky.social, and Prof Mark Wilkins, who famously invented the word "Proteome"

Schematic representation of the 14 human siglecs, 4 evolutionarily conserved on the left, with low sequence similarity, and 10 on the right sharing a higher sequence similarity to CD33. Siglec-6 is highlighted in yellow as it is the focus of this work. This is Figure 1 in the paper

When glycan recognition is linked to immune response, you need fine tuning! 😎

Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️

doi.org/10.1101/2025...

By deep characterisation of 186 N-glycan standards, we @thencfg.bsky.social have made the most comprehensive targeted LC-MS glycomic assay to date.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.

Resonated so well with so many speakers that it gets quoted by almost every planery talk that follows on. #Glyco27 @sugar-princess.bsky.social

Imagine if more countries fund glyco like Canada #Glyco27 #glycotime

📱 Don’t forget: our social media competition is live!

Follow us and use #Glyco27 when posting on LinkedIn, X (Twitter), or Bluesky for a chance to win a prize!

Let’s get the conversation going and have an amazing conference!

#Glycotime #Glycoscience #Glycobiology #Canada #YEG

Congratulations to @nickescott.bsky.social on his dual MCR prize from both AGS @ozglyco.bsky.social and APS for his work on bacterial glycosylation! #glycotime

Spatial mapping of the brain metabolome lipidome and glycome - Nature Communications Clarke et al. presents a framework for spatial analysis of the metabolome, lipidome, and glycome from a single tissue section using mass spectrometry imaging. Applying this approach, they revealed reg...

Spatial mapping of the brain metabolome lipidome and glycome #NatCommun #MassSpec www.nature.com/articles/s41...

Glycoproteins, Glycolipids, or Both: Why do Glycosyltransferases Recognize Different Acceptors? Glycosylation is an important post-translational modification catalyzed by glycosyltransferases (GTs), which comprise a large group of enzymes with diversified specificity. Most GTs show high specifi...

Nice #glycotime review that pulls together the emerging story of glycosyltransferase specificity for cellular substrates

chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

T-cell branched glycosylation as a mediator of colitis-associated colorectal cancer progression: a potential new risk biomarker in inflammatory bowel disease AbstractBackground and Aims. Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, established as a risk facto

academic.oup.com/ecco-jcc/art...

Happy to share our most recent publication on how T cells glycosylation mediates #IBD progression to colorectal cancer defining the risk of colitis-associated cancer.
#glycotime Great work @eduardalgomes.bsky.social @i3suporto.bsky.social @GlycanTrigger

Australian academics refuse to attend US conferences for fear of being detained ‘When academics fear travelling or partnering with US institutions, the impacts ripple through the entire global knowledge ecosystem,’ one says

There are great conferences and science in the US, but with the way things are right now, it’s too risky to attend.

In-depth plasma N-glycoproteome profiling using narrow-window data-independent acquisition on the Orbitrap Astral mass spectrometer Nature Communications - While DIA has advanced standard proteomics, its application in glycoproteomics has remained limited. Here, the authors present an optimized narrow-window DIA method for...

Just out by Shelley Jager: rdcu.be/edEnO. One of the fastest & deepest plasma glycoproteomics studies. Take home message; we even detect glycosylated cytokines and chemokines, but also still need better software. #glycotime @utrechtuniversity.bsky.social @bijvoet-centre.bsky.social

Glycocalyx dysregulation impairs blood–brain barrier in ageing and disease - Nature Disruption of mucin-domain glycoprotein expression and function in the endothelial glycocalyx are associated with ageing and Alzheimer’s disease, leading to dysregulated blood–brain barrier function.

Happy to share this new #glycotime work with collaborator Tony Wyss-Coray, led by grad student Sophia Shi, mucin loss at the BBB is associated with aging and cognitive decline, can be reversed by restoration with with gene therapy. Aging is a mucinopathy!

www.nature.com/articles/s41...

Picture this: your fav protein-protein interaction is *entirely* dependent on,
1) glycans 💥
2) the type of glycans 🤯💜
Check out this doi.org/10.1016/j.jb... 🔝 #glycotime fresh off the press in JBC led by Nicki Packer and Len Harrison 🇦🇺 + us 🇮🇪🇬🇧 + @ethangbaus.bsky.social 🇦🇺
🧵1/5

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments #JVisExp #MassSpec app.jove.com/t/67405/glyc...

A POGase is a huge advance in the glycomics field, which has typically relied on chemical or incomplete enzymatic releases (no Sia).
Expect a lot of multi-omic studies enabled by this (N-glycomics, O-glycomics, and proteomics) since the peptide backbone is left intact.
www.nature.com/articles/s41...

ANSMS 2025 Medal Recipients. Morrison Medal, Prof Nicki Packer from Macquarie University. Bowie Medal, A/Prof Shane Ellis from University of Wollongong.

🎉Announcing our ANZSMS 2025 Medalists 🎉

Congratulations to Prof Nicki Packer from Macquarie University and A/Prof Shane Ellis from University of Wollongong!

Join us at AUS-omics from May 18 - 21 to celebrate this wonderful achievement! 🏆

Read about our medalists here 👉 mailchi.mp/6c90f3d41712...

A robust glycomic study, can’t critique any aspect of the sample QC or methods used.
Really nice work.
#glycotime #TeamMassSpec

GlycoBioTec2025

Had a great time at GlycoBioTec2025 (www.mpi-magdeburg.mpg.de/glycobiotec2...), held once every 2 years, with a good amount of industry participants. Furthermore, food at Harnack Haus is fabulous!

Live imaging of the extracellular matrix with a glycan-binding fluorophore - Nature Methods Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...

Check it out, powerful new method for optical imaging of the live ECM, from @hhmijanelia.bsky.social Group Leader Kayvon Pedram #proudofalumni #glycotime

www.nature.com/articles/s41...

Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...

Our latest work on Siglec binding to sulfated glycans. Great help: @khookh.bsky.social, @pengwu.bsky.social ,@raflynn5.bsky.social. Fom 1st PhD graduate of the lab (Jaesoo Jung) and help from Edward, Zeinab, Jhon, Kelli, @tayloregray.bsky.social, and Chris from my lab.
pubs.acs.org/doi/10.1021/...