(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen!

Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵

A clump of green worm-like structures on a large, complex purple structure

This is an image of a human immune cell called a macrophage (purple) connecting with the bacteria Mycobacterium tuberculosis (green), which causes TB. #microscopy 🧪🔬

Image credit: Tony Fearns and Beren Aylan

How to delete Facebook, Instagram, and Threads | TechCrunch In the wake of Meta’s decision to remove its third-party fact-checking system and loosen content moderation policies, Google searches on how to delete

In the wake of Meta’s decision to remove its third-party fact-checking system and loosen content moderation policies, here's how to delete your Facebook, Instagram, and Threads accounts

I fear there is deep truth here.

Still our favorite #review! Unfortunately, as relevant today as when it was published, it is an excellent illustration of the #problems arising from the neglect of #ultrastructure and #electronmicroscopy in #cell-biology and the over-reliance on #fluorescence #microscopy.

doi.org/10.1016/0962...

Science in the age of selfies | PNAS Science in the age of selfies

#Science in the age of #selfies

A short #opinion article that we think hits a very important point!

Albert #Einstein: “an academic career, in which a person is forced to produce #scientific writings in great amounts, creates a #danger of intellectual superficiality”

www.pnas.org/doi/10.1073/...

Ants best humans at test of collective intelligence

"When people work together, they can achieve great things. But if they can’t talk, they’re not necessarily smarter than ants"

Original paper
www.pnas.org/doi/10.1073/...

News
www.science.org/content/arti...

Technically superb, live imaging experiments visualise intraflagellar transport in zebrafish ciliated tissues, unveil the surprising breadth of intraflagellar transport speed.
https://buff.ly/41UJjMS

snow under the electron microscope

sea cucumber skin under a microscope

Goldman Sachs asks in biotech research report: 'Is curing patients a sustainable business model?' Goldman Sachs warns sales from the most successful disease treatments are difficult to maintain.

We possess the technology to create products that nearly never break. We were doing it in the 70's & 80's.

Then came 'planned obsolescence' - intentionally designing products to become obsolete or break quickly within a set time frame.

This is that- but in reverse.

the world's smallest christmas tree made from diatoms

At my institution, they want to install profiles on our personal phones that will give IT root level access to our personal phones.

And allow them to remotely wipe our phones. Our personal phones.

Damn straight, take your work email off your phone. And remove your work profiles from your phones.

Instead of listing my publications, as the year draws to an end, I want to shine the spotlight on the commonplace assumption that productivity must always increase. Good research is disruptive and thinking time is central to high quality scholarship and necessary for disruptive research.

Urban Trees' Role in Education: Insights from Chicago's Ash Borer Infestation Discover how urban tree loss impacts education in cities, with new research from Chicago revealing surprising effects on students from low-income families.

Discover how urban tree loss impacts education in cities, with new research from Chicago revealing surprising effects on students from low-income families.

Das #pentaradio24 – Daten sind strukturierte Informationen, aber sind sie verlässlich? Bürokratien machen aus Menschen Datenpunkte. Was können wir tun, damit Daten wieder im Dienste der Menschen stehen? c3d2.de/news/pentara...

Ending OCSP Support in 2025 Earlier this year we announced our intent to provide certificate revocation information exclusively via Certificate Revocation Lists (CRLs), ending support for providing certificate revocation informa...

We're very disappointed Let's Encrypt is ending support for proper revocation checks via OCSP Must-Staple which is the only efficient, private and secure method not depending on a browser-specific service:

letsencrypt.org/2024/12/05/e...

No replacement is being offered for the feature.

What Makes a Tick Stick? Ticks form a stable structure around their mouth to stick to their hosts for days. Phase transitions of proteins in the tick saliva drive this adhesion.

This is fascinating! Ticks form a "cement cone" from their saliva that glues them to you. Now we know more about the chemistry of that bioadhesive process 🧪 www.the-scientist.com/what-makes-a...

Wideband laser speckle rheological microscopy reveals the viscoelastic landscape of biological tissues with high spatial resolution (25 micrometers) 👏

www.science.org/doi/10.1126/...

#Mechanobiology

An image from the now retracted paper, Figure 6B. It shows ten black and white microscopy photos, organized in two rows labeled Mock and si321+si359 and five columns labeled #1 through #5. I have marked three of the boxes with red, green and yellow boxes, showing unexpected overlaps.

This lab @tulanemedicine.bsky.social has 23 papers with @pubpeer.com comments and 7 retractions.
Yet, the Principal Investigator is still a professor at Tulane.
Is Tulane looking the other way, as long as the PI brings in the grants?
pubpeer.com/publications...

I'm gonna piss a few nerds off but this is an example of why a lightsheet microscope should be used to study organoids. The detection optics are great for imaging in XY but they suck axially. Sure, yeah, hocus pocus, RI matching, correction collar, blah blah, but it's physics, baby.

photography of a palm weevil that seems ready for a fight

The best science images of 2024 — Nature’s picks

www.nature.com/immersive/d4... 🧪

📷 close-up of the species Rhynchophorus ferrugineus, by Sherif Abdallah Ahmed/Nikon Small World Photomicrography Competition

Immunopathology in human tuberculosis The balance between protective and pathological immune responses shapes progression of Mycobacterium tuberculosis infection.

“In this review, we discuss the main manifestations, immunological players, and risk factors of TB immunopathology and highlight opportunities for medical intervention.” #ImmunoSky 🩺🧪

The full body of a parasitic copepod

A branch-like organ that is used to anchor the parasite to its host

The main body of the parasitic copepod that houses the reproductive and digestive organs. In this stage of its lifecycle, it has lost its limbs and antennae

Here’s another very interesting and common marine organism. They’re copepods, albeit, they look almost nothing like typical free-living copepods you may know.

This group starts as juveniles looking like most other copepods, but lose their limbs and antennae once they mature.

🦑🧪

Poliovirus keeps popping up in European wastewater, perplexing and worrying scientists Origins and spread of the vaccine-derived viruses remain mysterious

So far, no paralytic cases have been found, and the risk of a poliovirus outbreak in countries with high vaccination rates is deemed fairly low.

🧪🦠

www.science.org/content/arti...

Illustration with text: "Our Christmas gift idea", including a book cover that reads "Lebenskünstler" ("Remarkable") by Russ Hodge, featuring a nature-themed cover, flanked by illustrated portraits of a person [Russ Hodge] with white hair and a person [Kat Menschik] with blonde hair on a teal background.

Last-minute Christmas shopping? Not for you! We recommend “Remarkable. What animals can teach us about ourselves, the value of diversity, and saving the world,” by @mdc-berlin.bsky.social science writer Russ Hodge and artist Kat Menschik!

More details: www.mdc-berlin.de/news/news/wo...

It's #FluorescenceFriday and we celebrate the #HistoryOfMicroscopy: The paper that introduced PAINT was published on the 12th of December 2006 in the journal PNAS. Read the historic paper: t.co/fw3cQrnEhX

#SuperResolution #HistoryOfScience #SMLM #Nanoimger #Microscopy

Political Microtargeting by EU Commission illegal EDPS issues decision finding that the European Commission has illegally targeted advertisment on citizens.

EU Commission tried to influence political views in the fight over the heavily criticized #chatcontrol regulation: Political Microtargeting by EU Commission was illegal noyb.eu/en/political-microtarget... #Chatkontrolle

The risk of shortcutting in deep learning algorithms for medical imaging research - Scientific Reports Scientific Reports - The risk of shortcutting in deep learning algorithms for medical imaging research

We are rather skeptical about conclusions based on #AI-driven / #deeplearning #image #analysis (not towards an application as a tool). This viewpoint is supported by a recent study on medical imaging, which we believe can be applied to #bioimage data in general. #microscopy

doi.org/10.1038/s415...