#SfN25 is packed with amazing science and networking opportunities. Cannot wait! The power of coming together has never been more important.
#Neuroscience #Microscopy #2Photon #3Photon #1Photon

A nice z-stack through cortical layers containing plaques and structures that express vimentin, a cytoskeletal protein.

#microscopy #neuroscience #2Photon #fluorescence

We're having a great time at @mblscience.bsky.social Neurobiology, and @nsb-mbl.bsky.social courses! This time excited to showcase multiphoton microscope from Sutter, powered by the Chameleon Discovery LX femtosecond laser from Coherent Corp.

#microscopy #neuroscience #2Photon #STEM

Longitudinal (over 6 wk) #2Photon imaging of 🐭Spleen #GerminalCenter remodeling (TLR7 agonist)

PMOXA-passivated abdominal imaging window ⏬immunogenicity

Treg/TFr ◀️Foxp3-DTR-GFP
Follicular dendritic cell◀️αCD35-iFluor-647
Collagen◀️SHG
Mφ◀️Autofluorescence

bioRxiv 2025
www.biorxiv.org/content/10.1...

I highlight work from Na Ji's Lab at UC Berkeley. Her lab developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. The fig below shows in vivo iGluSnFR-A184S+ sensor imaging for mapping excitatory inputs. doi.org/10.1038/s415...
#neuroscience #2Photon

#2Photon calcium imaging in the hippocampus of awake pigeons cell.com/cell-reports...
by Simon Nimpf, Harris Kaplan, Greg Nordmann, Thomas Cushion, and David Keyas

#FluorescenceFriday #MicroscopyMonday #Neuroscience

Using a combination of slice #ephys and #2photon imaging the authors showed that activation of #LC noradrenergic neurons induces lateral inhibition via alpha2 adrenoreceptors.

Impact of photobleaching of fluorescent proteins on FRET measurements under two-photon excitation bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 Impact of photobleaching of fluorescent proteins on FRET measurements under two-photon excitation

🧑‍🔬 Dhruba P. Adhikari, Michael R. Stoneman, Valerica Raicu

📔 @biorxiv-cellbio.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #Microscopy #FRET #FRETmicroscopy #2Photon #Bioimaging

Rolled a G size gas cylinder the first time in about 20 years, as a part of the handling training. Last time I did this was back at @NIH to connect an LN2 cylinder to the @SpectraPhysics #Tsunami #2photon #laser...