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#AUTOFLUORESCENCE
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New preprint! Liver macrophages are highly autofluorescent cells, complicating analysis. @sabinedaemen.bsky.social developed a spectral flow approach to resolve #macrophage subsets in healthy vs #MASLD livers.
#FlowCytometry #SpectralFlow #Autofluorescence
doi.org/10.64898/202...

a cartoon of alice from alice in wonderland with the words " and it begins " below her ALT: a cartoon of alice from alice in wonderland with the words " and it begins " below her

Trying to pinpoint the exact cause, I chased #autofluorescence down a rabbit hole. By the time I emerged, I looked like the mad hatter, but had also created my first #rstats package Luciernaga. It automated identifying degrading #tandem #fluorophores, and isolating secondary autofluorescences . 7/n

New #UpdateCourseRewind2024: Use of #autofluorescence & #ICG #angiography for identifying #parathyroid glands & assessing perfusion w/ Drs. Phillip Ham III & Justin Huntington
Host: @emgooteemd.bsky.social
Full Ep: gcmd.co/43jvFlS
#pedsurg #SoMe4PedSurg
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@cincychildrens.bsky.social

Extra #autofluorescence (s), one hell of a drug😅 34-color panel, gated on live lymphocytes. Unmixed on the Aurora, left row with general AF extract, right column with an additional AF tag. Main AF had primary peak V7 detector, second AF (25% live cells) main peak was UV7 #spectral #flow #cytometry

Hello bluesky and happy #fluorescencefriday!
Today, we present this confocal cross section of the earthworm Lumbricus terrestris showing #autofluorescence. #TIMEBOW lifetime imaging was used to visualize differences in fluorescent lifetime in distinct colors.

#microscopy

This natural glow is called #autofluorescence and various molecules contribute to it (metabolism - related, components of extracellular matrix...). With age our tissues also accumulate lipofuscins, which are a jumble of metals, misfolded proteins and oxidised lipids, that fluoresce !

➡️

This natural glow is called #autofluorescence and various molecules contribute to it (components of extracellular matrix, metabolism-related molecules..). With age, our tissues accumulate lipofuscins, which are a jumble of metals, misfolded proteins and oxidised lipids, that fluoresce!

➡️