IntAct Portal EMBL-EBI

#FeaturedDataset: NeuroNet1.0 or "Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains". Massive neurodegeneration-focused y2h study from E. Wanker's lab @MDC_Berlin:

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: NeuroNet1.0 or "Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains". Massive neurodegeneration-focused y2h study from E. Wanker's lab @MDC_Berlin:

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: Falter-Braun group's "Extensive signal integration revealed by a phytohormone protein interactome map". Because life goes on beyond COVID19, check phytohormone effects on a dataset of hundreds of plant interactions:

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: Falter-Braun group's "Extensive signal integration revealed by a phytohormone protein interactome map". Because life goes on beyond COVID19, check phytohormone effects on a dataset of hundreds of plant interactions:

ebi.ac.uk/intact/query/p…

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing An outbreak of the novel coronavirus SARS-CoV-2, the causative agent of COVID-19 respiratory disease, has infected over 290,000 people since the end of 2019, killed over 12,000, and caused worldwide social and economic disruption[1][1],[2][2]. There are currently no antiviral drugs with proven efficacy nor are there vaccines for its prevention. Unfortunately, the scientific community has little knowledge of the molecular details of SARS-CoV-2 infection. To illuminate this, we cloned, tagged and expressed 26 of the 29 viral proteins in human cells and identified the human proteins physically associated with each using affinity-purification mass spectrometry (AP-MS), which identified 332 high confidence SARS-CoV-2-human protein-protein interactions (PPIs). Among these, we identify 67 druggable human proteins or host factors targeted by 69 existing FDA-approved drugs, drugs in clinical trials and/or preclinical compounds, that we are currently evaluating for efficacy in live SARS-CoV-2 infection assays. The identification of host dependency factors mediating virus infection may provide key insights into effective molecular targets for developing broadly acting antiviral therapeutics against SARS-CoV-2 and other deadly coronavirus strains. * HC-PPIs : High confidence protein-protein interactions PPIs : protein-protein interaction AP-MS : affinity purification-mass spectrometry COVID-19 : Coronavirus Disease-2019 ACE2 : angiotensin converting enzyme 2 Orf : open reading frame Nsp3 : papain-like protease Nsp5 : main protease Nsp : nonstructural protein TPM : transcripts per million [1]: #ref-1 [2]: #ref-2

#FeaturedDataset: #SARSCov2-human interactome from the @KroganLab! 332 interactions between 27 viral proteins and their human interactors, as described inData available on our website

ebi.ac.uk/intact/query/p… biorxiv.org/content/10.110…

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing An outbreak of the novel coronavirus SARS-CoV-2, the causative agent of COVID-19 respiratory disease, has infected over 290,000 people since the end of 2019, killed over 12,000, and caused worldwide social and economic disruption[1][1],[2][2]. There are currently no antiviral drugs with proven efficacy nor are there vaccines for its prevention. Unfortunately, the scientific community has little knowledge of the molecular details of SARS-CoV-2 infection. To illuminate this, we cloned, tagged and expressed 26 of the 29 viral proteins in human cells and identified the human proteins physically associated with each using affinity-purification mass spectrometry (AP-MS), which identified 332 high confidence SARS-CoV-2-human protein-protein interactions (PPIs). Among these, we identify 67 druggable human proteins or host factors targeted by 69 existing FDA-approved drugs, drugs in clinical trials and/or preclinical compounds, that we are currently evaluating for efficacy in live SARS-CoV-2 infection assays. The identification of host dependency factors mediating virus infection may provide key insights into effective molecular targets for developing broadly acting antiviral therapeutics against SARS-CoV-2 and other deadly coronavirus strains. * HC-PPIs : High confidence protein-protein interactions PPIs : protein-protein interaction AP-MS : affinity purification-mass spectrometry COVID-19 : Coronavirus Disease-2019 ACE2 : angiotensin converting enzyme 2 Orf : open reading frame Nsp3 : papain-like protease Nsp5 : main protease Nsp : nonstructural protein TPM : transcripts per million [1]: #ref-1 [2]: #ref-2

#FeaturedDataset: #SARSCov2-human interactome from the @KroganLab! 332 interactions between 27 viral proteins and their human interactors, as described inData available on our website

ebi.ac.uk/intact/query/p… biorxiv.org/content/10.110…

IntAct Portal EMBL-EBI

#FeaturedDataset: Final instance of the human interactome from the Vidal/Hill/Calderwood lab at @DanaFarber! Luck et al report over 160K interaction evidence and over 52K unique protein pairs. Data available on our website

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: Final instance of the human interactome from the Vidal/Hill/Calderwood lab at @DanaFarber! Luck et al report over 160K interaction evidence and over 52K unique protein pairs. Data available on our website

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: Mutations-related paper from Yu lab at @CornellRsrch! Fragoza et al test the impact of 2009 missense variants across 2185 protein-protein interactions. Data available on our websiteand on #IntActMutationsDS

ebi.ac.uk/intact/resourc… ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset: Mutations-related paper from Yu lab at @CornellRsrch! Fragoza et al test the impact of 2009 missense variants across 2185 protein-protein interactions. Data available on our websiteand on #IntActMutationsDS

ebi.ac.uk/intact/resourc… ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset Huttlin et al #BioPLEX data set finally finds its way into @intact_project: Over 58K interactions from interactome-scale AP-MS screening
(link:

ebi.ac.uk/intact/query/p…

IntAct Portal EMBL-EBI

#FeaturedDataset Huttlin et al #BioPLEX data set finally finds its way into @intact_project: Over 58K interactions from interactome-scale AP-MS screening
(link:

ebi.ac.uk/intact/query/p…

Europe PMC Europe PMC is an archive of life sciences journal literature.

IntAct release 221 #FeaturedDataset: Woodsmith et al, Nature Methods, 2017 . Deep-mutagenesis + interaction profiling. Over 10,000 #mutations and their effects on #proteininteractions within the BBsome in @intact_project:

ebi.ac.uk/intact/search/… europepmc.org/abstract/MED/2…

Europe PMC Europe PMC is an archive of life sciences journal literature.

IntAct release 221 #FeaturedDataset: Woodsmith et al, Nature Methods, 2017 . Deep-mutagenesis + interaction profiling. Over 10,000 #mutations and their effects on #proteininteractions within the BBsome in @intact_project:

ebi.ac.uk/intact/search/… europepmc.org/abstract/MED/2…

An orthogonal proteomic survey uncovers novel Zika virus host factors Nature - Integrative analyses identify host proteins that are modulated by Zika virus at multiple levels and provide a comprehensive framework for the understanding of Zika virus-induced changes to...

IntAct 218 release #FeaturedDataset is out @nature! "An orthogonal #proteomic survey uncovers novel Zika virus host factorsProteomics data from the same publication is available at @pride_ebi:

x.com/pride_ebi/stat… go.nature.com/2LYBVFT

An orthogonal proteomic survey uncovers novel Zika virus host factors Nature - Integrative analyses identify host proteins that are modulated by Zika virus at multiple levels and provide a comprehensive framework for the understanding of Zika virus-induced changes to...

IntAct 218 release #FeaturedDataset is out @nature! "An orthogonal #proteomic survey uncovers novel Zika virus host factorsProteomics data from the same publication is available at @pride_ebi:

x.com/pride_ebi/stat… go.nature.com/2LYBVFT

Europe PMC Europe PMC is an archive of life sciences journal literature.

Check out IntAct 213 release #FeaturedDataset! Winczura et al. in Cell Reports… on the function of ZC3H18 and its interaction with components of the CBC complex (@complexportal #CotM):

ebi.ac.uk/intact/query/p… europepmc.org/abstract/MED/2…

Europe PMC Europe PMC is an archive of life sciences journal literature.

Check out IntAct 213 release #FeaturedDataset! Winczura et al. in Cell Reports… on the function of ZC3H18 and its interaction with components of the CBC complex (@complexportal #CotM):

ebi.ac.uk/intact/query/p… europepmc.org/abstract/MED/2…

Europe PMC Europe PMC is an archive of life sciences journal literature.

Check out IntAct 213 release #FeaturedDataset! Winczura et al. in Cell Reportson the function of ZC3H18 and its interaction with components of the CBC complex (@complexportal #CotM).

europepmc.org/abstract/MED/2…

Europe PMC Europe PMC is an archive of life sciences journal literature.

Check out IntAct 213 release #FeaturedDataset! Winczura et al. in Cell Reportson the function of ZC3H18 and its interaction with components of the CBC complex (@complexportal #CotM).

europepmc.org/abstract/MED/2…

Check out IntAct 211 release #FeaturedDataset! Cell, Yang et al, 2016Over 3000 differential isoform interactions using y2h from the Vidal's lab

ebi.ac.uk/intact/query/p… europepmc.org/articles/PMC48…

Check out IntAct 211 release #FeaturedDataset! Cell, Yang et al, 2016Over 3000 differential isoform interactions using y2h from the Vidal's lab

ebi.ac.uk/intact/query/p… europepmc.org/articles/PMC48…