The #Bvitamin biotin is essential for cells to maintain normal #genefunction. Current understanding suggests that biotin plays a particularly significant role in #cellularfunction among #olderadults. #Biotin rich foods encompass eggs, lean meats, salmon, peanuts, & sunflower seeds. #healthyaging

💡 Why perturb genes?

By turning off genes or their protein products, we can see how a cell’s behavior or molecular signature changes — revealing clues about what those genes actually do. This work lays the foundation for understanding disease.

#CRISPR #GeneFunction

A genome-wide atlas of human cell morphology | Nature Methods A key challenge of the modern genomics era is developing empirical data-driven representations of gene function. Here we present the first unbiased morphology-based genome-wide perturbation atlas in human cells, containing three genome-wide genotype–phenotype maps comprising CRISPR–Cas9-based knockouts of >20,000 genes in >30 million cells. Our optical pooled cell profiling platform (PERISCOPE) combines a destainable high-dimensional phenotyping panel (based on Cell Painting) with optical sequencing of molecular barcodes and a scalable open-source analysis pipeline to facilitate massively parallel screening of pooled perturbation libraries. This perturbation atlas comprises high-dimensional phenotypic profiles of individual cells with sufficient resolution to cluster thousands of human genes, reconstruct known pathways and protein–protein interaction networks, interrogate subcellular processes and identify culture media-specific responses. Using this atlas, we identify the poorly

Explore the genome-wide atlas of human cell morphology: CRISPR-Cas9 knockouts for 20,000+ genes, 30M+ cells using PERISCOPE & Cell Painting! #GeneFunction 🎨🔬 PMID:39870862, Nat Methods 2025, @naturemethods @OTSociety https://doi.org/10.1038/s41592-024-02537-7 #Medsky #Pharmsky #RNA 🧪