Fig. 1. Polyamine biosynthesis in S. meliloti.
Divergent and overlapping roles of homospermidine and spermidine in Sinorhizobium meliloti physiology and symbiotic performance. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001668
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Arginine-rich peptides increase the total ROS. (a) Oxidative stress levels in E. coli and S. aureus after exposure to peptides at MIC for 4 h at 37°C. (b) Fluorescence images show the impact of D-R4F4 on E. coli intracellular ROS accumulation. (c) GO terms for molecular function associated with downregulated DEGs involved in oxidoreductase activity in E. coli after D-R4F4 peptide treatment for 24 h. (d) PPI network for downregulated DEGs identified in response to oxidative stress (STRING database).
D-amino acid substitution and cyclization enhance the stability and antimicrobial activity of arginine-rich peptides. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001657
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Killing of Coccidioides by drugs. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001666
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Fig. 1. Fundamental elements of DNA structure and topology.
Bacterial DNA supercoiling. Published in Microbiology as part of the Microbial Primers collection: https://doi.org/10.1099/mic.0.001667
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Overview of study design. (a) Pre-treatment with arrowroot and infection of T84 cells. (b) Treatment with arrowroot at POI of T84 cells.
Antimicrobial and anti-inflammatory properties of Maranta arundinacea extract against Campylobacter jejuni and Campylobacter coli in T84 cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001658 #MicrobioJ #PublishandRead
aacC1 synonymous variant design and constructs.
Hurdles to horizontal gene transfer: species-specific effects of synonymous variation and plasmid copy number determine antibiotic resistance phenotype. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001652 #MicrobioJ
Graphical abstract: Electron transfer pathways of Paracoccus denitrificans.
Read the latest Microbe Profile on Paracoccus denitrificans - a versatile model. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001644 #MicrobioJ #PublishandRead
Fig. 1. Schematic of experimental workflow. Schematic produced using Biorender.com.
Using a ‘one strain-many compounds’ approach to screen a collection of diverse fungi from Aotearoa New Zealand for antibacterial activity against Escherichia coli. Published Open Access and fee-free Microbiology: https://doi.org/10.1099/mic.0.001641
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Summary of mycobiomes in health or disease status in different body sites.
The human mycobiome: a critical yet understudied component of health and disease. New review available to read in Microbiology: doi.org/10.1099/mic....
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The defect of the ΔtatABC mutant adhesion is specifically due to the lack of the tatABC operon.
Invasive Acinetobacter baumannii ABC141 strain relies on the twin-arginine translocation export system for adhesion to host cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic....
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Gene deletion mutant exhibits increased permeability to propidium iodide and ethidium bromide. Exponentially growing GB11 WT, its isogenic ∆cas9 gene deletion mutant and the ∆cas9::cas9-complemented mutant were exposed to vancomycin on BA media (n=4), washed and stained with ethidium bromide (a) (left panel) or propidium iodide (b) (right panel). Fluorescence was measured at 605 nm for ethidium bromide and at 617 nm for propidium iodide. P<0.05 was considered statistically significant. **, P<0.01; ***, P<0.001; ns, not significant. Data are shown as mean±sem. N=3–6 samples per scattergram in each graph. Circles represent for GB11 the WT; squares the GB11Δcas9 mutant; and triangles the GB11Δcas9::cas9-complemented mutant strain.
Cas9 modulates Campylobacter jejuni virulence traits inside intestinal epithelial cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic....
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TEM visualization and schematic model of R-pyocin structure and killing mechanism in P. aeruginosa.
Read the latest Microbial Primer on the R-pyocins of Pseudomonas aeruginosa. Available now in Microbiology: doi.org/10.1099/mic....
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Graphs showing metabolic gene clusters of G. adspersum.
Siderophores and secondary metabolites produced by Ganoderma adspersum. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
PCA of fluorophore uptake in E. coli and A. baylyi exposed to antibiotics. (a) Intracellular versus cell membrane-acting antibiotics. (b) A. baylyi versus E. coli. Fluorescence signals from 23 fluorophores (Table S2) in cells exposed to 19 different antibiotics. Data generated from four or more biological replicates. Components consisted of species (E. coli and A. baylyi), antibiotics, fluorophore, events (median), signal (median) and signal ratio (fluorescence emission from samples exposed to antibiotic versus samples without exposure to antibiotics) and target type for each antibiotic (Tables S1 and S2).
Rapid accumulation of fluorophores and fast kill identify drugs with bactericidal effects against Gram-negative bacteria. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Identification of the Y. lipolytica CSAD gene using E. coli with optimized genetic mutations (ΔcysA, ΔssuD and ΔcysC). The E. coli strain CR2SC, harbouring the pTA-YlC1 plasmid, was streaked onto Davis minimal agar plates with or without 0.5 mM l-cysteic acid. Both plates contained ampicillin to ensure plasmid maintenance. The cells were incubated at 37 °C for 3 days. As a control, CR2SC harbouring the empty vector pTA was cultured under the same conditions to confirm the specificity of the observed activity.
Identification of the Yarrowia lipolytica cysteine sulfinic acid decarboxylase gene using a newly developed method with optimized Escherichia coli combinations of mutant alleles. Read now in Microbiology: doi.org/10.1099/mic.... #MicrobioJ
CRISPR-Cas systems are divided into two classes (class 1 and class 2) and six main types (types I to VI), differentiated by their protein architecture and mechanisms of action. (b) The response consists of three stages: (I) adaptation – the insertion of foreign genetic material into the bacterial cell, followed by its recognition and cleavage by proteins from the adaptation module (black box, PAM-protospacer adjacent motif) to generate spacers. (II) Biogenesis: Transcription of the CRISPR locus. The resulting pre-CRISPR RNA (pre-crRNA) is processed by the Cas9/RNase III complex, which recognizes repeated sequences. The process produces mature crRNAs that subsequently pair with trans-activating CRISPR RNA (tracrRNA) to form guide RNA (gRNA). (III) Interference: When the genetic material is put back into the cell, if it has the right protospacer and PAM sites, the Cas/gRNA complexes recognize it and cut it.
A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
E. coli survival and conjugation in the presence of different seaweed substrates. (a) E. coli conjugation rates (transconjugants/donors×recipients×time) and (b) fold change in population densities after 48 h in seawater in the presence of one of three desiccated seaweeds or a plastic control. Asterisks denote a significant difference between treatments, *P<0.05; ****P<0.0001. For all statistical model values, see Tables S2 and S3. For absolute E. coli densities, see Fig. S2. NA – transconjugant numbers below the limit of detection in at least one replicate.
Seaweed exposure modulates Escherichia coli plasmid conjugation rate. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Black and white microscopic image of Streptomyces from 1955 showing detailed filamentous structures in microbiology research.
Side-by-side electron micrographs comparing Streptomyces aerial hyphae from 1955 and 2022, highlighting structural details.
Infrequent pathogens have been an important focus of research for decades, as captured in #MicrobioJ since 1947. For example, Streptomyces: see this 1955 photo of S. scabies and these 2022 images of S. venezuelae, which you can read more about here: doi.org/10.1099/mic....
Streptomyces formicae was isolated from Tetraponera penzigi plant-ants and has bioactivity against Gram-positive pathogens due to the production of formicamycins.
Read the latest Microbe Profile on Streptomyces formicae KY5: an ANT-ibiotic factory. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Streptomyces formicae was isolated from Tetraponera penzigi plant-ants and has bioactivity against Gram-positive pathogens due to the production of formicamycins.
Read the latest Microbe Profile on Streptomyces formicae KY5: an ANT-ibiotic factory. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
New guidance was published to aid academics/SMEs with interpreting the MHRA “Regulatory considerations for therapeutic use of bacteriophages in the UK”, published earlier this year.
Read the interpretation guidance here: doi.org/10.1099/mic.... #MicrobioJ
Colonies of M. marinum wild-type (WT), mutant (ΔmmpL12) and complemented (ΔmmpL12-C) strains on 7H9+ agar plates obtained after incubation at 30 °C for 1 week.
MmpL12 transports lipooligosaccharides and impacts virulence in Mycobacterium marinum. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Primary biochemical characteristics, growth kinetics and safety parameters of isolates obtained from the lab-prepared fermented beverages. Black colour indicates positive; white colour means negative.
Assessing lactococcal and enterococcal strains derived from traditionally fermented kefir and nabeez for their prospects as probiotics. Learn more in Microbiology: doi.org/10.1099/mic.... #MicrobioJ
(a) Genes in S. Typhimurium that affect susceptibility to NaCl, KCl, SL and SN. (b) Genes mapped to metabolic models showing respiration (left) and membrane polysaccharide synthesis (right).
Common food preservatives induce an oxidative stress response in Salmonella enterica serovar Typhimurium. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Saturated fatty acids inhibit the growth of Enterococcus faecalis.
Enterococcus faecalis requires unsaturated fatty acids to overcome toxicity of environmental saturated fatty acids. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Did you know food-derived natural products may reveal how antimicrobial resistance plasmids spread in Enterobacterales? Celebrate World Food Day by reflecting on how our diet affects the world. Read more in Microbiology: doi.org/10.1099/mic.... #MicrobioJ #WorldFoodDay
Colony morphotypes identified from experimentally evolved lineages after a 10-day incubation at 20 °C on bacteriological agar supplemented with 1% w/v tryptone, 20 µg ml−1 Congo red and 40 µg ml−1 Coomassie brilliant blue. Data are shown as representative colony dimensions in pixels, n=4.
Cyclic-di-GMP signalling mutants drive ecological succession and self-generated diversity in experimentally evolved biofilms of Pseudomonas aeruginosa. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Fig. 1. Cytotoxicity is altered in transposon mutants of TCSs in S. aureus. To quantify cytotoxicity, the bacterial supernatant from transposon mutants in each non-essential HK and RR gene was incubated with THP-1 cells, and the percentage of dead cells was quantified using trypan blue exclusion. THP-1 killing was calculated relative to the THP-1 killing of JE2 (WT) in each assay. Each dot represents one biological replicate (n = 3), error bars represent the sd and statistical significance was determined using multiple t-tests with an FDR of 1% applied. Significant hits, which had an adjusted P-value ≤0.01, are highlighted in purple.
Phosphate sensing by PhoPR regulates the cytotoxicity of Staphylococcus aureus. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
![Fig. 1.
Genome-wide distribution of CRP as determined by ChIP-seq. (a) The circular plots show binding of CRP across the EAEC 042 chromosome and plasmid pAA2. Genes are shown as grey blocks, the G+C content is shown as a dark blue and cyan plot and CRP binding is shown as an orange plot. (b) The binding motif obtained from 332 CRP peak sequences using MEME suite [24]. (c) Histogram showing positions of CRP binding motifs relative to gene start codons. Four peaks were excluded that fall outside the range of the x-axis. The pie chart shows the proportion of CRP binding sites that are located within genes or in intergenic regions.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:mmksquuwyw2qm3qdnslkrodx/bafkreighvwjngr5opq2f3dnroz5r7dvrwmxv2q4p35zeocrgnigikudik4)
Fig. 1. Genome-wide distribution of CRP as determined by ChIP-seq. (a) The circular plots show binding of CRP across the EAEC 042 chromosome and plasmid pAA2. Genes are shown as grey blocks, the G+C content is shown as a dark blue and cyan plot and CRP binding is shown as an orange plot. (b) The binding motif obtained from 332 CRP peak sequences using MEME suite [24]. (c) Histogram showing positions of CRP binding motifs relative to gene start codons. Four peaks were excluded that fall outside the range of the x-axis. The pie chart shows the proportion of CRP binding sites that are located within genes or in intergenic regions.
Genome-wide mapping of cAMP receptor protein binding in enteroaggregative Escherichia coli reveals targeting of virulence-associated genes. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
Fig. 1. Role of IAV in P. aeruginosa biofilm dispersal. PA01 (a), non-CF (b) and CF (c) biofilms were exposed to IAV (MOI-1) at 37 °C for 3 h in the presence or absence of HNECs. The biofilm biomass compared to clinical isolates of PA exposed to media, media+IAV and cells. Data are expressed as mean+sem. Each dot represents 1 replicate; N=9 for experimental conditions; N=3–4 for control.
Effects of influenza A infection on liberation of bacteria from biofilms and inflammatory response in an in vitro model of chronic rhinosinusitis. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead
