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Fig. 1.
Polyamine biosynthesis in S. meliloti.

Fig. 1. Polyamine biosynthesis in S. meliloti.

Divergent and overlapping roles of homospermidine and spermidine in Sinorhizobium meliloti physiology and symbiotic performance. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001668

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Preview
Genome sequences of distinct genotypes of bacterial pathogen Xanthomonas euvesicatoria pv. euvesicatoria from pepper (Capsicum annuum L.) in Serbia This Technical Resource presents genome sequence data for three strains of the bacterial pathogen Xanthomonas euvesicatoria pv. euvesicatoria (Xeu) collected in Serbia. We isolated these strains from pepper crops showing bacterial spot symptoms in 2016 at the municipality of Irig, in the Srem district. The presented data comprise raw sequencing reads and annotated, contig-level genome assemblies. We checked for the presence of sequences of known type-3 secretion system (T3SS) effector genes and plasmid-like sequences. Phylogenomic reconstruction revealed that the three strains fell in the...

Genome sequences of distinct genotypes of bacterial pathogen Xanthomonas euvesicatoria pv. euvesicatoria from pepper (Capsicum annuum L.) in Serbia.
Published Open Access and fee-free in ACMI using a Publish and Read agreement: https://doi.org/10.1099/acmi.0.001138.v4

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Fig. 2.
Symptoms of CTD include stunting, distorted growth and leaves with curling, crumpling, yellowing and vein swelling and purpling in selected plant species 15 days after agroinoculation with the infectious clone of GV-CTD-MT the genomic DNA of BraCTV.

Fig. 2. Symptoms of CTD include stunting, distorted growth and leaves with curling, crumpling, yellowing and vein swelling and purpling in selected plant species 15 days after agroinoculation with the infectious clone of GV-CTD-MT the genomic DNA of BraCTV.

How a chance finding and high-throughput sequencing helped unmask the probable causal agent of Brazilian curly top, a plant disease that disappeared over 70 years ago. Published Open Access and fee-free in JGV: https://doi.org/10.1099/jgv.0.002188

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Fig. 1.
EE design and phenotypic evaluation of initial variants and final populations of PVY.

Fig. 1. EE design and phenotypic evaluation of initial variants and final populations of PVY.

The replicative fitness and virulence of potato virus Y evolve differently in pepper lines with different levels of resistance and tolerance. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002208

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Arginine-rich peptides increase the total ROS. (a) Oxidative stress levels in E. coli and S. aureus after exposure to peptides at MIC for 4 h at 37°C. (b) Fluorescence images show the impact of D-R4F4 on E. coli intracellular ROS accumulation. (c) GO terms for molecular function associated with downregulated DEGs involved in oxidoreductase activity in E. coli after D-R4F4 peptide treatment for 24 h. (d) PPI network for downregulated DEGs identified in response to oxidative stress (STRING database).

Arginine-rich peptides increase the total ROS. (a) Oxidative stress levels in E. coli and S. aureus after exposure to peptides at MIC for 4 h at 37°C. (b) Fluorescence images show the impact of D-R4F4 on E. coli intracellular ROS accumulation. (c) GO terms for molecular function associated with downregulated DEGs involved in oxidoreductase activity in E. coli after D-R4F4 peptide treatment for 24 h. (d) PPI network for downregulated DEGs identified in response to oxidative stress (STRING database).

D-amino acid substitution and cyclization enhance the stability and antimicrobial activity of arginine-rich peptides. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001657

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Fig. 5. V. cholerae cases associated with foreign travel outside of the UK, where a specific country was stated (n=868). Countries are colour-coded in proportion to the frequency of cases associated, i.e. the higher the frequency of cases, the darker the colour.

Fig. 5. V. cholerae cases associated with foreign travel outside of the UK, where a specific country was stated (n=868). Countries are colour-coded in proportion to the frequency of cases associated, i.e. the higher the frequency of cases, the darker the colour.

Public health surveillance of Vibrio cholerae in travellers returning to the United Kingdom. Published Open Access and fee-free in JMM using a Publish and Read agreement: https://doi.org/10.1099/jmm.0.002121

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Preview
Killing of Coccidioides by drugs Coccidioidomycosis has long been regarded as one of the most difficult mycoses to eradicate in patients with progressive infection. There are few published studies of inhibition of Coccidioides in vitro, comparing the results with various drugs as measured by minimal inhibitory concentration (MIC). An ability to kill Coccidioides, at achievable concentrations in the host, may be necessary to produce a cure. Five hundred fifty-seven killing assays of Coccidioides clinical isolates in vitro, with minimum fungicidal concentrations (MFCs), are reported here. Heterogeneity of MFCs for all drugs...

Killing of Coccidioides by drugs. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001666

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Fig. 1. rCedV-Luc screening setup and identification of candidate compounds.

Fig. 1. rCedV-Luc screening setup and identification of candidate compounds.

Screening of FDA-approved drugs using a recombinant Cedar virus to improve treatment options for Nipah virus infection. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002195
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Fig. 1. Images of B. burgdorferi culture isolated from blood plasma and CSF of five children with LD. Images were taken at the first timepoint (day of incubation) when viable spirochetes were detected. Abbreviation: CSF, cerebrospinal fluid.

Fig. 1. Images of B. burgdorferi culture isolated from blood plasma and CSF of five children with LD. Images were taken at the first timepoint (day of incubation) when viable spirochetes were detected. Abbreviation: CSF, cerebrospinal fluid.

Streamlining Borrelia burgdorferi cultivation using quantitative PCR screening. Published Open Access and fee-free in JMM using a Publish and Read agreement: https://doi.org/10.1099/jmm.0.002123
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Overview of study design. (a) Pre-treatment with arrowroot and infection of T84 cells. (b) Treatment with arrowroot at POI of T84 cells.

Overview of study design. (a) Pre-treatment with arrowroot and infection of T84 cells. (b) Treatment with arrowroot at POI of T84 cells.

Antimicrobial and anti-inflammatory properties of Maranta arundinacea extract against Campylobacter jejuni and Campylobacter coli in T84 cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001658 #MicrobioJ #PublishandRead

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Cells of FW153T (a) and FW199T (b). Bars are 5 µm.

Cells of FW153T (a) and FW199T (b). Bars are 5 µm.

Rhizorhabdus antheiae sp. nov. and Sphingomonas eleionomae sp. nov., new aerobic anoxygenic phototrophs isolated from a Manitoban marsh. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: https://doi.org/10.1099/ijsem.0.007018 #IJSEM #PublishandRead

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AhV capsid structure. (a) A section of a representative micrograph of AhV VLPs and 2D class averages. Scale bar=100 nm. (b) The 2.4 Å resolution 3D reconstruction of AhV, colored according to quasi-conformer, with monomer A colored pink and monomer B colored grey. The capsid surface (left) and a central slice through the capsid (right) are shown. (c) The atomic model of AhV, showing the model for a single asymmetric unit from the bottom (left) and the side (right) with the monomers colored as in panel (b).

AhV capsid structure. (a) A section of a representative micrograph of AhV VLPs and 2D class averages. Scale bar=100 nm. (b) The 2.4 Å resolution 3D reconstruction of AhV, colored according to quasi-conformer, with monomer A colored pink and monomer B colored grey. The capsid surface (left) and a central slice through the capsid (right) are shown. (c) The atomic model of AhV, showing the model for a single asymmetric unit from the bottom (left) and the side (right) with the monomers colored as in panel (b).

Atkinsonella hypoxylon virus capsid structure highlights the diversity of capsid proteins among the Partitiviridae. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002209 #JGV #PublishandRead

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Abstract white line forming an M shape next to bold Microbiology Society text on a dark blue background with green and orange dots.

Abstract white line forming an M shape next to bold Microbiology Society text on a dark blue background with green and orange dots.

Evolved populations of Listeria monocytogenes related to biofilm formation and biocide stress in the context of food production environment niches. Published Open Access and fee-free in MGen using a Publish and Read agreement: https://doi.org/10.1099/mgen.0.001611 #MGen #PublishandRead

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NJ tree showing the phylogenetic relationships of strains AW1-3T and AW1-7T with closely related taxa based on 16S rRNA gene sequences. Bootstrap values greater than 70% (based on 1,000 replicates) are indicated at the corresponding nodes. Filled circles (●) denote nodes also supported by ML and MP algorithms. Sphingobacterium spiritivorum ATCC 33861T (M58778) was used as the outgroup. The scale bar indicates 0.01 nucleotide substitutions per site.

NJ tree showing the phylogenetic relationships of strains AW1-3T and AW1-7T with closely related taxa based on 16S rRNA gene sequences. Bootstrap values greater than 70% (based on 1,000 replicates) are indicated at the corresponding nodes. Filled circles (●) denote nodes also supported by ML and MP algorithms. Sphingobacterium spiritivorum ATCC 33861T (M58778) was used as the outgroup. The scale bar indicates 0.01 nucleotide substitutions per site.

Mucilaginibacter aureus sp. nov. and Mucilaginibacter sediminis sp. nov., isolated from wetland soil. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: https://doi.org/10.1099/ijsem.0.007042 #IJSEM #PublishandRead

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Fig. 1: Appearance of L. garvieae isolate on horse blood agar after 24 h incubation at 37 °C in 5% CO2.

Fig. 1: Appearance of L. garvieae isolate on horse blood agar after 24 h incubation at 37 °C in 5% CO2.

Lactococcus garvieae prosthetic aortic and mitral valve endocarditis with multiple embolic complications: a case report. Published Open Access and fee-free in ACMI using a Publish and Read agreement: https://doi.org/10.1099/acmi.0.001149.v3 #AccessMicro #PublishandRead

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Fig. 2: Heatmap of pairwise ANI values of the 23 strains and their closely related taxa.

Fig. 2: Heatmap of pairwise ANI values of the 23 strains and their closely related taxa.

Ten novel psychrophilic Flavobacterium species from Tibetan Plateau glaciers define a cryospheric lineage with global cold-origin relatives. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: https://doi.org/10.1099/ijsem.0.007021 #IJSEM #PublishandRead

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Graphical abstract: Electron transfer pathways of Paracoccus denitrificans.

Graphical abstract: Electron transfer pathways of Paracoccus denitrificans.

Read the latest Microbe Profile on Paracoccus denitrificans - a versatile model. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: https://doi.org/10.1099/mic.0.001644 #MicrobioJ #PublishandRead

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Fig. 4.
Three-dimensional scheme of the structural proteins of SVA and evolutionary adaptations/changes in the Brazilian SVA sequences. Amino acids highlighted in yellow in the predicted protein structures are undergoing evolution over the years (2015–2022). VP3 protein is represented in purple, VP2 in cyan, VP1 in green and VP4 in blue. In the legend frames, the most frequent amino acid residues in each position and in the specific year are shown.

Fig. 4. Three-dimensional scheme of the structural proteins of SVA and evolutionary adaptations/changes in the Brazilian SVA sequences. Amino acids highlighted in yellow in the predicted protein structures are undergoing evolution over the years (2015–2022). VP3 protein is represented in purple, VP2 in cyan, VP1 in green and VP4 in blue. In the legend frames, the most frequent amino acid residues in each position and in the specific year are shown.

Genetic diversity and evolutionary dynamics of Senecavirus A in Brazil. Published Open Access and fee-free in JGV: https://doi.org/10.1099/jgv.0.002212

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Fig. 2.
Most HBV splice sites are distinct from host splice sites.

Fig. 2. Most HBV splice sites are distinct from host splice sites.

Decoding the interconnected splicing patterns of hepatitis B virus and host using large language and deep learning models. Published Open Access and fee-free in MGen using a Publish and Read agreement: https://doi.org/10.1099/mgen.0.001616
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Fig. 3.
Scanning electron micrographs of clusters of RSM42T.

Fig. 3. Scanning electron micrographs of clusters of RSM42T.

Rothia similimucilaginosa sp. nov., isolated from the human nasal cavity. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: https://doi.org/10.1099/ijsem.0.007024 #IJSEM #PublishandRead

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Core genome phylogeny of 91 M. plutonius isolates from US apiaries collected between 2019 and 2023. Isolates with blue text published from previous studies were retrieved from NCBI. Isolates used in this study are outlined in red, and phylogeny between study isolates is outlined on the right. DAT561 and S1 represent atypical and typical reference strains, respectively, obtained from NCBI. Numbers and arrows represent bootstrap values.

Core genome phylogeny of 91 M. plutonius isolates from US apiaries collected between 2019 and 2023. Isolates with blue text published from previous studies were retrieved from NCBI. Isolates used in this study are outlined in red, and phylogeny between study isolates is outlined on the right. DAT561 and S1 represent atypical and typical reference strains, respectively, obtained from NCBI. Numbers and arrows represent bootstrap values.

Potential confounding factors in currently used antibiotic susceptibility assays for the honey bee pathogen Melissococcus plutonius. . Published Open Access and fee-free in JMM using a Publish and Read agreement: doi.org/10.1099/jmm....

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Graphical representation of the EAV outbreak cases in Devon and Dorset. Devon and Dorset dressage stallions are hypothesized to have close contact during transport while horses were moved for shows during 2018 equine gatherings or particularly for breeding purposes. Solid lines – most likely transmission; dotted lines – alternative transmission route.

Graphical representation of the EAV outbreak cases in Devon and Dorset. Devon and Dorset dressage stallions are hypothesized to have close contact during transport while horses were moved for shows during 2018 equine gatherings or particularly for breeding purposes. Solid lines – most likely transmission; dotted lines – alternative transmission route.

Genetic characterization of equine arteritis virus associated with outbreaks in the UK, 2019. Published Open Access and fee-free in JGV using a Publish and Read agreement: doi.org/10.1099/jgv....

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Internalization of 3867K-EVs by recipient avian cells. The 100K-EVs isolated from 3867K cultures were labelled with the green fluorescence dye PKH-67. As a negative control, PBS underwent the same staining procedure. The labelled EVs were then incubated with two sets of recipient cells

Internalization of 3867K-EVs by recipient avian cells. The 100K-EVs isolated from 3867K cultures were labelled with the green fluorescence dye PKH-67. As a negative control, PBS underwent the same staining procedure. The labelled EVs were then incubated with two sets of recipient cells

Extracellular vesicles released from Marek’s disease virus-transformed T-cells impact immune cell proliferation. Published Open Access and fee-free in JGV using a Publish and Read agreement: doi.org/10.1099/jgv....

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The defect of the ΔtatABC mutant adhesion is specifically due to the lack of the tatABC operon.

The defect of the ΔtatABC mutant adhesion is specifically due to the lack of the tatABC operon.

Invasive Acinetobacter baumannii ABC141 strain relies on the twin-arginine translocation export system for adhesion to host cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic....

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Experimental schematic diagram (a) and changes in serum oxidative stress and inflammation-related markers in LW and LW×LR crossbred sows during late pregnancy (b–i). LW_LR, large white×landrace crossbred sows; ns, No significance; *P<0.05; **P<0.01.

Experimental schematic diagram (a) and changes in serum oxidative stress and inflammation-related markers in LW and LW×LR crossbred sows during late pregnancy (b–i). LW_LR, large white×landrace crossbred sows; ns, No significance; *P<0.05; **P<0.01.

Gut microbiota and bile acid profiles in purebred vs. crossbred sows: links to oxidative stress and inflammation in late gestation. Published Open Access and fee-free in MGen using a Publish and Read agreement: doi.org/10.1099/mgen...

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NJ tree showing the phylogenetic relationships of strains S174ᵀ and W118ᵀ with closely related type strains based on 16S rRNA gene sequences.

NJ tree showing the phylogenetic relationships of strains S174ᵀ and W118ᵀ with closely related type strains based on 16S rRNA gene sequences.

Flagellimonas ulvae sp. nov. and Flagellimonas rhodophyticola sp. nov., isolated from marine algae. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: doi.org/10.1099/ijse...

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A chronology of the major changes that took place in the history of Acinetobacter in the period from 1900 to 1980.

A chronology of the major changes that took place in the history of Acinetobacter in the period from 1900 to 1980.

History of the taxonomy of Acinetobacter: the emergence of hospital-adapted species of global health concern. Published Open Access and fee-free in IJSEM using a Publish and Read agreement: doi.org/10.1099/ijse...

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Identification of SMIs. Chemical structures of MCCB5, CB6 and MJM364 and predicted docking poses. Three hydrophobic hotspots were identified as important for the NP:VP35 interaction. Compounds were screened in silico using the eHiTs exhaustive docking engine, AutoDock cluster analysis and pose prediction software or de novo designed (see also Fig. S1A, available in the online Supplementary Material).

Identification of SMIs. Chemical structures of MCCB5, CB6 and MJM364 and predicted docking poses. Three hydrophobic hotspots were identified as important for the NP:VP35 interaction. Compounds were screened in silico using the eHiTs exhaustive docking engine, AutoDock cluster analysis and pose prediction software or de novo designed (see also Fig. S1A, available in the online Supplementary Material).

Discovery of three small-molecule inhibitors targeting Ebolavirus genome replication and transcription. Published Open Access and fee-free in JGV using a Publish and Read agreement: doi.org/10.1099/jgv....

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Gene deletion mutant exhibits increased permeability to propidium iodide and ethidium bromide. Exponentially growing GB11 WT, its isogenic ∆cas9 gene deletion mutant and the ∆cas9::cas9-complemented mutant were exposed to vancomycin on BA media (n=4), washed and stained with ethidium bromide (a) (left panel) or propidium iodide (b) (right panel). Fluorescence was measured at 605 nm for ethidium bromide and at 617 nm for propidium iodide. P<0.05 was considered statistically significant. **, P<0.01; ***, P<0.001; ns, not significant. Data are shown as mean±sem. N=3–6 samples per scattergram in each graph. Circles represent for GB11 the WT; squares the GB11Δcas9 mutant; and triangles the GB11Δcas9::cas9-complemented mutant strain.

Gene deletion mutant exhibits increased permeability to propidium iodide and ethidium bromide. Exponentially growing GB11 WT, its isogenic ∆cas9 gene deletion mutant and the ∆cas9::cas9-complemented mutant were exposed to vancomycin on BA media (n=4), washed and stained with ethidium bromide (a) (left panel) or propidium iodide (b) (right panel). Fluorescence was measured at 605 nm for ethidium bromide and at 617 nm for propidium iodide. P<0.05 was considered statistically significant. **, P<0.01; ***, P<0.001; ns, not significant. Data are shown as mean±sem. N=3–6 samples per scattergram in each graph. Circles represent for GB11 the WT; squares the GB11Δcas9 mutant; and triangles the GB11Δcas9::cas9-complemented mutant strain.

Cas9 modulates Campylobacter jejuni virulence traits inside intestinal epithelial cells. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic....

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Patient-reported symptoms at each presentation. At each clinic visit, patients with long COVID were invited to report any symptoms they associated with their long COVID. Patients were allowed to report multiple symptoms, so total frequency of all symptoms is greater than the number of patients. If the patient reported resolution of symptoms, they were counted as ‘resolved’. At visit 1 (n=33), patients had experienced one confirmed infection. At visit 2 (n=33), patients had been vaccinated at least once and 12/33 experienced a confirmed reinfection. By visit 3 (n=15), patients had been vaccinated at least twice and 8/13 experienced a confirmed reinfection. By visit 3 (n=13) patients had been vaccinated at least three times and 10/13 experienced a confirmed reinfection.

Patient-reported symptoms at each presentation. At each clinic visit, patients with long COVID were invited to report any symptoms they associated with their long COVID. Patients were allowed to report multiple symptoms, so total frequency of all symptoms is greater than the number of patients. If the patient reported resolution of symptoms, they were counted as ‘resolved’. At visit 1 (n=33), patients had experienced one confirmed infection. At visit 2 (n=33), patients had been vaccinated at least once and 12/33 experienced a confirmed reinfection. By visit 3 (n=15), patients had been vaccinated at least twice and 8/13 experienced a confirmed reinfection. By visit 3 (n=13) patients had been vaccinated at least three times and 10/13 experienced a confirmed reinfection.

Robust antibody and T cell responses tracked longitudinally in patients with long COVID. Published Open Access and fee-free in JGV using a Publish and Read agreement: doi.org/10.1099/jgv....

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