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Fig. 2.
Symptoms of CTD include stunting, distorted growth and leaves with curling, crumpling, yellowing and vein swelling and purpling in selected plant species 15 days after agroinoculation with the infectious clone of GV-CTD-MT the genomic DNA of BraCTV.

Fig. 2. Symptoms of CTD include stunting, distorted growth and leaves with curling, crumpling, yellowing and vein swelling and purpling in selected plant species 15 days after agroinoculation with the infectious clone of GV-CTD-MT the genomic DNA of BraCTV.

How a chance finding and high-throughput sequencing helped unmask the probable causal agent of Brazilian curly top, a plant disease that disappeared over 70 years ago. Published Open Access and fee-free in JGV: https://doi.org/10.1099/jgv.0.002188

#PublishandRead #JGenVirol

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Fig. 1.
EE design and phenotypic evaluation of initial variants and final populations of PVY.

Fig. 1. EE design and phenotypic evaluation of initial variants and final populations of PVY.

The replicative fitness and virulence of potato virus Y evolve differently in pepper lines with different levels of resistance and tolerance. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002208

#PublishandRead #JGenVirol

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Jobs View the current job vacancies at the Microbiology Society.

Join JGV as an Editor! We are searching for individuals with a strong background in positive-strand RNA viruses, fungal viruses and insect viruses. The deadline to apply is Monday 2 April. For more information follow this link: https://microb.io/Vacancies

#JGenVirol #Opportunities

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Fig. 3. CPEs in Vero cells caused by the three isolates of Mpox. Mpox-CH1 (a, b) and Mpox-CH2 (c, d) exhibited similar CPEs, and Mpox-CH3 (e, f) showed marked fusogenicity at 3–4 d.p.i. (arrow). Scale bar, 100 µm.

Fig. 3. CPEs in Vero cells caused by the three isolates of Mpox. Mpox-CH1 (a, b) and Mpox-CH2 (c, d) exhibited similar CPEs, and Mpox-CH3 (e, f) showed marked fusogenicity at 3–4 d.p.i. (arrow). Scale bar, 100 µm.

Identification of an extremely large-scale genomic rearrangement in the Mpox virus while retaining replicative capacity. Learn more in JGV: https://doi.org/10.1099/jgv.0.002215

#JGenVirol

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Fig. 1. rCedV-Luc screening setup and identification of candidate compounds.

Fig. 1. rCedV-Luc screening setup and identification of candidate compounds.

Screening of FDA-approved drugs using a recombinant Cedar virus to improve treatment options for Nipah virus infection. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002195
#PublishandRead #JGenVirol

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Fig. 2: Mid-tracheal sections of birds infected with IBV with acute lesions of varying severity

Fig. 2: Mid-tracheal sections of birds infected with IBV with acute lesions of varying severity

Australian strains of the avian coronavirus infectious bronchitis virus predominantly target the respiratory tract rather than the kidneys in specific-pathogen-free chickens. Published Open Access and fee-free in JGV using a Publish and Read agreement: https://doi.org/10.1099/jgv.0.002213 #JGenVirol

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Fig. 4.
Three-dimensional scheme of the structural proteins of SVA and evolutionary adaptations/changes in the Brazilian SVA sequences. Amino acids highlighted in yellow in the predicted protein structures are undergoing evolution over the years (2015–2022). VP3 protein is represented in purple, VP2 in cyan, VP1 in green and VP4 in blue. In the legend frames, the most frequent amino acid residues in each position and in the specific year are shown.

Fig. 4. Three-dimensional scheme of the structural proteins of SVA and evolutionary adaptations/changes in the Brazilian SVA sequences. Amino acids highlighted in yellow in the predicted protein structures are undergoing evolution over the years (2015–2022). VP3 protein is represented in purple, VP2 in cyan, VP1 in green and VP4 in blue. In the legend frames, the most frequent amino acid residues in each position and in the specific year are shown.

Genetic diversity and evolutionary dynamics of Senecavirus A in Brazil. Published Open Access and fee-free in JGV: https://doi.org/10.1099/jgv.0.002212

#JGenVirol #PublishandRead

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Transcript-level validation of proteomic changes by RT-qPCR. Transcript levels of RCN2, APOB, XPNPEP3, ALDH4A1 and ENO3 were analysed by RT-qPCR in PHH at 8 dpi. Gene expression was normalized to GAPDH. HBV infection was confirmed by quantifying total HBV RNA (top left), shown on a logarithmic scale and normalized to the HBV+BLV group. All other mRNA levels are shown relative to uninfected controls (Ctrl). Data represent mean±sd from a single experiment; individual points represent three replicates.

Transcript-level validation of proteomic changes by RT-qPCR. Transcript levels of RCN2, APOB, XPNPEP3, ALDH4A1 and ENO3 were analysed by RT-qPCR in PHH at 8 dpi. Gene expression was normalized to GAPDH. HBV infection was confirmed by quantifying total HBV RNA (top left), shown on a logarithmic scale and normalized to the HBV+BLV group. All other mRNA levels are shown relative to uninfected controls (Ctrl). Data represent mean±sd from a single experiment; individual points represent three replicates.

Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytes. View the full article in JGV: doi.org/10.1099/jgv.... #JGenVirol

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Spatial distribution and genetic diversity of H1N1pdm09 in Italian swine, 2009–2024. (a) Distribution of H1N1pdm09 swine genome sequences (n=45) by Italian macro-region. (b) Annual count of newly obtained H1N1 swine genome sequences by Italian macro-region. Colours represent different macro-regions: Northeast (light blue), Northwest (blue grey), Central (green), South (black) and Insular (white). Missing years had no genomes.

Spatial distribution and genetic diversity of H1N1pdm09 in Italian swine, 2009–2024. (a) Distribution of H1N1pdm09 swine genome sequences (n=45) by Italian macro-region. (b) Annual count of newly obtained H1N1 swine genome sequences by Italian macro-region. Colours represent different macro-regions: Northeast (light blue), Northwest (blue grey), Central (green), South (black) and Insular (white). Missing years had no genomes.

From North to South: transmission dynamics of H1N1pdm09 swine influenza A viruses in Italy. Explore this further in JGV: doi.org/10.1099/jgv.... #JGenVirol

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IBA1 staining of cervical lymph nodes in RABV+ and RABV− dogs. (a) Representative images of IBA1-stained (in red) cervical lymph nodes from RABV− and RABV+ dogs; scale bar: 500 µm (b) Histological scoring of IBA1 staining (1–10 scale) in RABV− dogs and RABV+. All graphs display mean values, with error bars representing sd.

IBA1 staining of cervical lymph nodes in RABV+ and RABV− dogs. (a) Representative images of IBA1-stained (in red) cervical lymph nodes from RABV− and RABV+ dogs; scale bar: 500 µm (b) Histological scoring of IBA1 staining (1–10 scale) in RABV− dogs and RABV+. All graphs display mean values, with error bars representing sd.

Immunohistochemical investigation of canine lymph nodes collected during a rabies outbreak in South Africa. Available to read in JGV: doi.org/10.1099/jgv.... #JGenVirol

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Phylogenetic tree of Troyka LTR retrotransposons based on the entire Pol protein. Branches are coloured based on the host phylogeny of Troyka. Three lineages from ray-finned fishes are indicated with arcs V1–V3. The original phylogenetic trees and protein alignments are available as Data S2 and S3. The species abbreviations in Troyka names are shown in Table S1.

Phylogenetic tree of Troyka LTR retrotransposons based on the entire Pol protein. Branches are coloured based on the host phylogeny of Troyka. Three lineages from ray-finned fishes are indicated with arcs V1–V3. The original phylogenetic trees and protein alignments are available as Data S2 and S3. The species abbreviations in Troyka names are shown in Table S1.

Troyka represents a unique lineage of virus-like retroelements. Get the details in JGV: doi.org/10.1099/jgv.... #JGenVirol

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For those of you working on tailed #phages, this is the virus realm for you!
#Duplodnaviria #virus #taxonomy

The Profile in #JGenVirol provides the citable reference. More details in the chapter on the ICTV website: ictv.global/report/chapt...

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Current taxonomy of the realm Duplodnaviria.

Current taxonomy of the realm Duplodnaviria.

Check out the latest ICTV Virus Taxonomy Profile on Duplodnaviria. Published in JGV as part of the ICTV Virus Taxonomy Profiles collection: doi.org/10.1099/jgv.... #JGenVirol

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Understanding the mechanisms of mitochondrial rewiring during viral infections. Published Open Access and fee-free in JGV using a Publish and Read agreement: doi.org/10.1099/jgv.... #JGenVirol #PublishAndRead

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Jobs View the current job vacancies at the Microbiology Society.

Final Week to apply for #JGenVirol Editor Position! We are seeking candidates with expertise in vaccines, antivirals, and plant viruses. If you have editorial experience in these areas, apply by 31st July: microb.io/Vacancies

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Fig. 1.
Mosquitoes collected from September 2020 to April 2022. (a) Map of mosquito sampling locations. Each sampling location, shown as dark blue polygons, contains one to nine trapping sites. Trapping sites with QBV detected are shown as orange diamonds, while sampling locations with QBV detections are in bold. (b) Temporal distribution of mosquitoes collected.

Fig. 1. Mosquitoes collected from September 2020 to April 2022. (a) Map of mosquito sampling locations. Each sampling location, shown as dark blue polygons, contains one to nine trapping sites. Trapping sites with QBV detected are shown as orange diamonds, while sampling locations with QBV detections are in bold. (b) Temporal distribution of mosquitoes collected.

Spatiotemporal prevalence and characterization of the lineage I insect-specific flavivirus, Quang Binh virus, isolated from Culex gelidus mosquitoes in Singapore. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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Fig. 5.
Transmission electron microscopic image of C6/36 cells infected with KEVOOV. White arrows indicate KEVOOV particles. (a) KEVOOV particles surrounding the vacuoles. (b) KEVOOV particles in the cell cytoplasm.

Fig. 5. Transmission electron microscopic image of C6/36 cells infected with KEVOOV. White arrows indicate KEVOOV particles. (a) KEVOOV particles surrounding the vacuoles. (b) KEVOOV particles in the cell cytoplasm.

Isolation and genetic characterization of a novel Kevo orbivirus and a strain of Mobuck virus from Ochlerotatus communis mosquitoes in Finland. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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Jobs View the current job vacancies at the Microbiology Society.

#JGenVirol is looking to appoint an Editor-in-Chief. The successful candidate will help develop the editorial strategy and new content for the journal. Applications close on 29 August: microb.io/Vacancies

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Jobs View the current job vacancies at the Microbiology Society.

Only 2 weeks remaining to apply for the Editor role at #JGenVirol! If you have previous editorial experience in handling vaccines, antivirals, and plant viruses submissions, please apply! Deadline closes on July 31 - microb.io/Vacancies

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Jobs View the current job vacancies at the Microbiology Society.

Join #JGenVirol as an Editor! We are searching for individuals with a strong background in vaccines, antivirals, and plant viruses. The deadline to apply is 31st July. For more information follow this link: microb.io/Vacancies

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Fig. 3.
Evolution and characteristics of GII.17 norovirus variants detected in humans. (a) Phylogenetic relationship of GII.17 noroviruses inferred using VP1 amino acid sequences through a maximum-likelihood method using mega v11 [111]. Each colour on the branch denotes different clusters of GII.17 noroviruses; cluster A represented by CS-E1/2002 (blue), cluster B represented by Katrina/2005 (green), cluster C represented by Kawasaki323/2014 (orange), cluster D represented by Kawasaki308/2015 (purple) and a recently emerged new cluster represented by Romania/2021 (red).

Fig. 3. Evolution and characteristics of GII.17 norovirus variants detected in humans. (a) Phylogenetic relationship of GII.17 noroviruses inferred using VP1 amino acid sequences through a maximum-likelihood method using mega v11 [111]. Each colour on the branch denotes different clusters of GII.17 noroviruses; cluster A represented by CS-E1/2002 (blue), cluster B represented by Katrina/2005 (green), cluster C represented by Kawasaki323/2014 (orange), cluster D represented by Kawasaki308/2015 (purple) and a recently emerged new cluster represented by Romania/2021 (red).

The saga to monitor and control norovirus: the rise of GII.17. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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Binomial names for virus species: the rediscovery of an old idea The International Committee on Taxonomy of Viruses now mandates that all virus species names be presented in a binomial format. This requirement replaces the various naming formats that have been…

Binomial names for virus species: the rediscovery of an old idea. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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Graphic shows all seven of Microbiology Society's journals, on a navy and turquoise background. Title reads "Discover our publishing portfolio".

Graphic shows all seven of Microbiology Society's journals, on a navy and turquoise background. Title reads "Discover our publishing portfolio".

Looking to publish your manuscript? Find out what you need to consider when choosing a journal to publish to @andybritalian.bsky.social , Head of Scientific Programmes at the Microbiology Society, will be at stand 524 ready to talk to you. #MicrobioJ #JGenVirol #mmc2025UK @royalmicrosoc.bsky.social

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Novel endornaviruses infecting Phytophthora cactorum that attenuate vegetative growth, promote sporangia formation and confer hypervirulence to the host oomycete. Learn more in #JGenVirol: doi.org/10.1099/jgv....

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Selection and validation of ALSV-LAMP primers. M, DNA Marker DL2000 (TaKaRa, China); N, no template control; P, LAMP assay using cDNA of ALSV genome as template; FP, LAMP assay using non-ALSV cDNA as template. (A) The effectiveness of LAMP primer set selection was evaluated through 1% gel electrophoresis. Three different sets of candidate LAMP primers were subjected to test. (B) The accuracy of the third primer set was confirmed by false-positive verification. This step validated the credibility and practicality of the chosen third primer set, as evident from the results of 1% gel electrophoresis. The reaction mixture comprised 12.5 µl of 2×Lamp Master Mix, 0.8 µM each of FIP and BIP, 0.2 µM each of F3 and B3, 0.16 U µl−1 of DNA Polymerase, 2 µl of template DNA and 5 µl of ddH2O.

Selection and validation of ALSV-LAMP primers. M, DNA Marker DL2000 (TaKaRa, China); N, no template control; P, LAMP assay using cDNA of ALSV genome as template; FP, LAMP assay using non-ALSV cDNA as template. (A) The effectiveness of LAMP primer set selection was evaluated through 1% gel electrophoresis. Three different sets of candidate LAMP primers were subjected to test. (B) The accuracy of the third primer set was confirmed by false-positive verification. This step validated the credibility and practicality of the chosen third primer set, as evident from the results of 1% gel electrophoresis. The reaction mixture comprised 12.5 µl of 2×Lamp Master Mix, 0.8 µM each of FIP and BIP, 0.2 µM each of F3 and B3, 0.16 U µl−1 of DNA Polymerase, 2 µl of template DNA and 5 µl of ddH2O.

Development of a loop-mediated isothermal amplification assay for the rapid detection of Alongshan virus. Published in #JGenVirol: doi.org/10.1099/jgv....

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NUP153 binds to HIV CA hexamers even without CbM.1, but this binding is blocked by PF74. (a) Diagram and experiment showing how NUP153c sticks to CA hexamer tubes, with and without CbM.1. Proteins were detected in different parts of the sample using Western blot. (b) Diagram and graph showing that PF74 prevents NUP153c from binding to CA hexamers by blocking key sites.

NUP153 binds to HIV CA hexamers even without CbM.1, but this binding is blocked by PF74. (a) Diagram and experiment showing how NUP153c sticks to CA hexamer tubes, with and without CbM.1. Proteins were detected in different parts of the sample using Western blot. (b) Diagram and graph showing that PF74 prevents NUP153c from binding to CA hexamers by blocking key sites.

The identification of a novel interaction site for the human immunodeficiency virus capsid on nucleoporin 153. Published Open Access and fee-free using a Publish and Read agreement: doi.org/10.1099/jgv.... #OpenAccess #JGenVirol #PublishAndRead

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Chart displaying overall prevalence of Omicron lineages circulated in Qatar during May and September 2022.

Chart displaying overall prevalence of Omicron lineages circulated in Qatar during May and September 2022.

The impact of pre-existing immunity on the emergence of within-host immune-escape mutations in Omicron lineages. Read the full article in #JGenVirol: doi.org/10.1099/jgv....

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Emergence, migration and spreading of the high pathogenicity avian influenza virus H5NX of the Gs/Gd lineage into America. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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Myosin IXB protects immune cells from virus infection. Find out more in #JGenVirol:
doi.org/10.1099/jgv....

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A region of mumps virus nucleoprotein affects defective interfering particle production. Published #OpenAccess and fee-free in #JGenVirol using a #PublishAndRead agreement:
doi.org/10.1099/jgv....

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