10/n Here’s what we found:
📌Chemo induces activated NECTIN2+ M1-like macrophages and exhausted, immune-checkpoint-positive CD8+ T cells, both co-localizing in the peritumoral stroma.
📌M1-like macrophages drive T cell exhaustion in #Myelonets, confirmed at the gene expression level.

5/n On the other hand, chemo reshapes the spatial landscape of #HGSC, transforming an spatially confined immunophenotype into a more infiltrated one. CD8+ T cells migrate from their “private” neighbourhoods toward cancer cell-hubs and #Myelonets.

2/n Spatial and molecular profiling of 117 #HGSC tumors pre- and post-chemo revealed a dynamic tumor ecosystem🔍. Using a high-plexed (30+🚨) antibody panel and #t-CycIF we identified #myelonets, interconnected networks of myeloid cells, as key drivers CD8+ T cell exhaustion upon chemotherapy.