Luminescence Lifetime Imaging: Key Concepts and Advances in the Image Acquisition, Processing, and Analysis Pipeline There is rapidly growing interest in applying luminescence lifetime imaging, i.e., FLIM and PLIM (fluorescence and phosphorescence lifetime imaging microscopy, respectively), across natural and life sciences. This includes developing new chemistries for luminescence lifetime read out, new instrumentation and data analysis approaches, as well as diverse applications in chemical, environmental and life sciences. The methodology captures the luminescence lifetime, an intrinsic fingerprint that is sensitive to the local physicochemical environment and specific molecular interactions, making it a valuable quantitative imaging technique for tissue characterization, live-cell metabolic studies, materials science studies, and more. Luminescence lifetime imaging is both robust and reproducible when properly executed but demands careful experimental design and substantial user expertise in terms of choice of imaging modality, instrumentation parameters, image acquisition settings and subsequent image processing and analysis. Therefore, this review addresses the principal determinants of imaging data and reviews practical methods and state-of-the-art advances to address them. Concurrently, this provides a foundation to discuss and highlight the current frontiers and future directions of this important imaging modality.

New review on #luminescence #lifetime #imaging - #FLIM and #PLIM
Massive work by flimagin3d.com PhD students Rishi Harkhoe and Gabriele Ferrari. Nice collaboration with Ruslan Dmitriev.
Check it out🤓

MSCA-DN flimagin3d
@moorefound.bsky.social

pubs.acs.org/doi/10.1021/...

✅SEXTA-FEIRA🥳🪩🍸
✅DIA MUNDIAL DO RÁDIO💞📻💞
✅DIA DE TER UM NOME DIFERENTE 😁
✅1️⃣3️⃣📍
✅CARNAVAL🎉🎭 🥸 🎊 BATENDO À 🚪...🧳... ☺️
✅VÉSPERA DO MEU #MÊSVERSÁRIO🎂
✅VÉSPERA DO DIA MUNDIAL DO #AMOR❤

💁‍♀️

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Só VEM - o dia, e também esse álbum da Mallu❣HeHe

✨ #plim! ✨

🔸️

#AMO❗💖

🎊 🤸🏻‍♀️ #sExToU❕🤸🏻‍♀️ 🎉

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#DTTIS2025 - Kamalika Datta and Lennart Weingarten from @unibremen.bsky.social and @dfki.bsky.social presenting our papers at #DTTIS dttis2025.uniwa.gr @dsc-ub.bsky.social #verification #synthesis #Plim #PolyVer

#DTTIS2025 - Kamalika Datta from @unibremen.bsky.social and @dfki.bsky.social presents our paper on "A Complete Synthesis and Verification Approach for MAGIC Based In-Memory Computing" dttis2025.uniwa.gr @dsc-ub.bsky.social #verification #synthesis #Plim #MAGIC #LiM

#10deJulho #DiaMundialDaLEI

de acordo com a ferramenta de I.A. do Google, sociedades criam leis para que, sob regras, haja PAZ e BEM ESTAR na convivência entre os seus membros.

Portanto, se você anda infringindo as Leis, não pergunte por quê ninguém quer conviver com você. Rs

#plim!

asa

Esse bom! para as "negras deles"!!
canalha do sistema do #Plim #Plim!!
😎💪.A luta continua!🚩L/13