Left: Schematic representation of the glycerol metabolism of PCF trypanosomes. This figure shows glycerol metabolism leading to the excreted end products, succinate and acetate in the glycosomes and the mitochondrion, respectively, while gluconeogenesis (production for G6P from G3P and DHAP) is represented in red. The oxidative and non-oxidative branches of the pentose-phosphate pathway (PPP) are highlighted in blue and purple, respectively. The three enzymes addressed in this study (PFK, FBPase, and SBPase), possibly responsible for production of F6P from F1,6BP, are boxed. Right: Images show the glycosomal (top) and cytosolic (bottom) localization of MaLionR-Myc-GPDH and MaLionG by immunofluorescence assays on the OEMaLionR-Myc-GPDH.i and OEMaLionG cell lines, respectively, by using an anti-aldolase immune serum as a glycosomal marker.
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc