🌾 dLbCas12a-RRV-intron platform: dual optimization (G146R/D156R/R182V variants + #Arabidopsis introns)
#RiceCBE: 32.73% avg (vs 15.47% ttLbCas12a), 81.58% bi-allelic at L8
#RiceABE: 51.87% at OsACCase → herbicide resistance
TTTV PAM: 52-63%, VTTV: 11-22%
Poplar: 0.47-11.87%
#Cas12a #PlantBE