🌾 dLbCas12a-RRV-intron platform: dual optimization (G146R/D156R/R182V variants + #Arabidopsis introns)
#RiceCBE: 32.73% avg (vs 15.47% ttLbCas12a), 81.58% bi-allelic at L8
#RiceABE: 51.87% at OsACCase → herbicide resistance
TTTV PAM: 52-63%, VTTV: 11-22%
Poplar: 0.47-11.87%
#Cas12a #PlantBE

🦠 100× boost in algae #CRISPR #knockout via dsNHO "decoys"
Co-delivery of 24bp non-homologous DNA sequesters KU proteins → forces MMEJ pathway → 0.29% → 44.5% efficiency in Chlamydomonas.
Works with #Cas9 & #Cas12a.
Read the full study:
onlinelibrary.wiley.com/doi/10.1111/...

New CRISPR tool enables more seamless gene editing — and improved disease modeling Yale scientists have developed a CRISPR technology that can assess genetic interactions on a host of immunological responses to multiple diseases, including cancer.

A new tool developed in the lab of Dr. Sidi Chen, CRISPR #Cas12a, enables more seamless gene editing and improved disease modeling, including genetic disease in the liver, #lungcancer, and #skincancer.
bit.ly/4hwYpg4
@yaleschoolofmed.bsky.social

Australian researchers enhance next-generation gene-editing technologies for cancer and medical research The study, supported by Phenomics Australia, advances gene editing capabilities for cancer and medical research through unprecedented genetic manipulations using gene-editing enzyme Cas12a. This story...

Australian researchers enhance next-generation gene-editing technologies for cancer and medical research.

phenomicsaustralia.org.au/australian-r...

#NCRISImpact #CRISPR #Cas12a #Cancer #research

@ceo.onjcri.org.au #AndrewKueh @onjcri.org.au @wehi-research.bsky.social @genentech.bsky.social

CRISPR technology: A decade of genome editing is only the beginning A review discusses the current state of CRISPR-mediated genetic manipulation in human cells, animals, and plants and considers its future potential.

#Medsky🧪 #immunosky #Academicsky overview the origins & utility of #CRISPR-based #genomeediting,
www.science.org/doi/10.1126/...
And What’s the Difference Between #Cas9 and #Cas12a #Nucleases?
www.the-scientist.com/what-s-the-d...