Pro tip! As much as I like the 55deg #Snouty microscope configuration (previous post) it's hard to align and keep aligned!
-> Consider using pre-designed O2-O3 optomechanics and an eyepiece to help avoid collisions during the set up!
github.com/amsikking/SO...
Hands down my favorite #Snouty microscope configuration!
-> The air objective with a 55deg light-sheet. It's not the highest resolution version, but the convenience of the air objective, the large field of view and the steep sectioning of the light-sheet make it a pleasure to use!
#AIRR #miOPM
Forgot I'm supposed to hashtag things. Here is so people can find it: #snouty #lightsheet
@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy!
Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.
#snouty
If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go!
Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy.
#snouty
❤️🔬 Exciting developments discussed at The Future of AI in Microscopy and Image Analysis meeting, organised by COMPARE at the University of Birmingham!
Excellent hosting by Davide Calebiro and team, and lovely to see Edalat Radfar's tour of our UoB #Snouty, and the robotic arm that feeds it!
It's the last #KingSnout ever! 😭
I'm looking forward to what's next in the #snouty objective lens line from ASI and Special Optics. Onwards to bigger and better things!
#snoutclub
@jsdaniel02.bsky.social @amsikking.bsky.social
Good news! You can now buy the @thorlabs.bsky.social QS20X Ø20 mm galvo with λ/4 flatness and E02 coating!
-> Great for scanning AND projecting volumes in a single-objective light-sheet (SOLS) microscope! #Snouty!
Part: G-N-2257
Description: "1D Galvo System, Dielectric, 400-750 nm, X-Orientation"
Who else has a #Snouty objective? I currently have 25 members in #SnoutClub! But I think there's a LOT MORE out there... Anyone else care to join?
-> Just reply here with a pic of your #Snouty and I'll add you!
Why? It helps us promote and develop the technology!
amsikking.github.io/snoutclub/
Pretty happy about this side view of mitochondria in an U2OS.
Pretty good axial resolution for an OPM, and a lightsheet in general.
#Microscopy #LightSheet
#Snouty
Also a big shoutout to Calico for entrusting us with some glass.
#Snouty
