Engineered TnpB via RNP-DMS

reRNA hinge region + protein hotspots (WED: N4/L172; RuvC: P282/E302/I304)

Tobacco: 55% indels (vs. <1% WT) = 50× boost
Rice: 29.3%, Pepper: 10%
HEK293T: 23-42%

<400 aa → AAV-compatible

TnpB-KYLI/VGIRL variants

📄 www.nature.com/articles/s41...

#CRISPR #TnpB

A headline from a bioRxiv research paper titled "Efficient transgene-free multiplexed genome editing via viral delivery of an engineered TnpB." It features authors from the labs of Jennifer A. Doudna and Steven E. Jacobsen. The image shows healthy Arabidopsis plants and mentions the breakthrough in using the engineered Ymu1-WFR variant for high-efficiency editing.

Big leap for plant breeding! 🧬 Jennifer Doudna & Steven Jacobsen’s teams just dropped a new system for transgene-free, multiplexed genome editing.
#CRISPR #PlantSci #Genomics #TnpB #AgTech

Directed evolution of compact RNA-guided nucleases for homology-directed repair in mammalian cells RNA-guided nucleases enable DNA editing and offer promise for treating genetic diseases, particularly when used for precise sequence replacement. However, many of the most effective enzymes, such as S...

Sometimes #Cas9 is too dang big. But mini Cas proteins are bad at both NHEJ & HDR. PhD student Fedor Gorbenko used mammalian cell evolution for *HDR* to make REALLY super #Cas12f1 and #TnpB. As good as or better than Cas9! Base editors included! www.biorxiv.org/cgi/content/short/2025.10.27.684765v1