Astrocytic LCN2 drives neuronal dysfunction in epilepsy by suppressing tunnel nanotube-mediated mitochondrial transfer via NLRP3 inflammasome activation Epileptic seizures disrupt brain homeostasis not only by directly challenging neuronal excitability but also by impairing the intricate astrocyte-neur…

Targeting LCN2/NLRP3 axis inhibits pyroptosis and aids mitochondrial rescue, exerting dual anti-epileptic therapeutic effects through Tunneling nanotube-mediated astrocyte-neuron mitochondria transfer #neuroscience #tunnelingnanotubes #cell-cellcommunication
www.sciencedirect.com/science/arti...

Thats interesting!
Must read.
I vote for transfer via #TunnelingNanotubes of course.

#TunnelingNanotubes are membranous extensions of cells that mediate cell contact-dependent intercellular transport of organelles and viruses. In our recent study of SARS-CoV-2 in tissue from patients, we found them in post-infected samples from matched tissue specimens. www.cell.com/iscience/ful...

MitoTracker transfers from astrocytes to neurons independently of mitochondria The neuroprotective transfer of mitochondria from astrocytes to neurons has been primarily investigated by labelling astrocytic mitochondria with the dye MitoTracker. Here we report that MitoTracker transfers to neurons from both astrocytes and astrocyte-conditioned media, independently of mitochondrial transfer. Our observations should prompt an essential re-evaluation of the literature concerning astrocyte-neuron mitochondrial transfer and in other systems in which contact-independent transfer has been observed using mitochondrial dyes. ### Competing Interest Statement The authors have declared no competing interest. MSD (United Kingdom), https://ror.org/004nn4n27, PRJ_20446 DEVINE MSD MRC The Francis Crick Institute, https://ror.org/04tnbqb63, CC2206

Wow!
Is (some of) the literature on #mitochodria transfer via #tunnelingnanotubes simply an artefact of mitotracker transfer?

www.biorxiv.org/content/10.1...

Also, soapbox moment: #TunnelingNanotubes and intercellular transfer of #mitochondria are not new concepts. At all.
They both have been known and identified nearly two decades ago.
I will discuss anywhere and at anytime during the course of #AACR25.
Thank you.

Tunneling nanotube–like structures regulate distant cellular interactions during heart formation In the developing mammalian heart, the endocardium and the myocardium are separated by so-called cardiac jelly. Communication between the endocardium and the myocardium is essential for cardiac morpho...

Awesome!
Tunneling nanotubes are getting to the mainstream high profile journals!
(21 years after the paper that coined the name #TunnelingNanotubes was published in Science)
#cytoskeleton

www.science.org/doi/10.1126/...

Beautiful imagery.
Cell Protrusions for the win in this February cover image of Shigella toxin inducing filopodia, in @naturemicrobiol.bsky.social

Viruses, bacteria, and other sources of cellular stress induce changes in cellular morphology - including #tunnelingnanotubes and others as well.

#TunnelingNanotubes and Intercellular Communication finally getting some recognition in 2025! I hope the trend continues for grant reviews (for those quarters where that is still occurring).

quite interesting!
#tunnelingnanotubes #mitochondria #cancer 🧪

Post-doctoral Positions Wanted: Talented applicants to study intercellular mRNA trafficking in vitro and in vivo and its application in RNA therapeutics Relevant works: Haimovich et al. 2017 PNAS http://www.pnas.org/content/114/46/E9873.full Dasgupta et al. 2023 eLife https://elifesciences.org/articles/83584 Haimovich et al. 2024 bioRxiv https://www.biorxiv.org/content/10.1101/2024.11.06.622258v1 Contact: Prof. Jeffrey Gerst Dept. of Molecular Genetics jeffrey.gerst@weizmann.ac.il www.weizmann.ac.il/molgen/Gerst/

💥 Reminder: 💥
We are still looking for a postdoc (we actually have enough funds for two!) to work on this promising project.
Details in the ad and the alt text
🧪 🧬 🔬 🐁
#RNASky #RNABiology #Cytoskel #TunnelingNanotubes

Pancreatic cancer cells exchange ribosomes through tunnelling nanotubes Background Pancreatic ductal adenocarcinoma (PDAC) is considered as one of the deadliest types of cancer. Tunnelling nanotubes (TNTs) are thin, membranous, intercellular communication structuresobserv...

How did I miss this preprint, posted in June?
Looks really intersting.
They suggest transfer of mRNA, ribosomes & translating ribosomes through #TunnelingNanotubes in pancreatic cancer, promoted by chemo.
Added to the reading pile.
#RNASky #RNABiology #Cytoskel 🧪🔬

www.biorxiv.org/content/10.1...

Lymphocytic choriomeningitis arenavirus utilises intercellular connections for cell to cell spread - Scientific Reports Scientific Reports - Lymphocytic choriomeningitis arenavirus utilises intercellular connections for cell to cell spread

oh nice.
A virus that is associated with fatal neurologic disease in humans is spreading between cells via #TunnelingNanotubes -like connections.
#cytoskel #virosky 🧪

www.nature.com/articles/s41...

I'm not 😔
I was planning to, but no RNA session, and my proposed session on #tunnelingnanotubes was not accepted.
it's too far and expensive to come just for a poster and no relevant sessions.

Are #TunnelingNanotubes considered part of the microenvireonment?
If you agree that they are, please add me to the list.
Thanks!

#TunnelingNanotubes are long filamentous actin-based cell protrusions that create communication networks between cells, especially in cancer. Here, an especially long nanotube transmits GFP-expressing oncolytic virus, a mechanism for propagation of the bystander effect.

www.cell.com/molecular-th...

I wanted to make a TNTs starter pack for #TunnelingNanotubes, #Cytoneme just for the fun of it.
But as far as I can tell it's only me, @labogden.bsky.social & @erichall.bsky.social and Bluesky requires at least 8 for a pack...
I hope the people I know from Twitter will join us here.

I'm reposting the thread about our latest paper for the benefit of my 300+ new followers(!)
I hope it'll interest you
🧪 #RNASky #RNABiology #tunnelingnanotubes

I made a starter pack for cytoskeleton people
#cytoskeleton #microtubules #actin #cilia #filopodia #tunnelingnanotubes

go.bsky.app/TF83cpi

a person in a yellow jacket is standing in front of a pipe that says get tubed on it ALT: a person in a yellow jacket is standing in front of a pipe that says get tubed on it

but by relatively under-studied cellular structures called #tunnelingnanotubes or TNTs for short. These ar long thin, actin-based open-ended tubes that connect cells and can transfer organnelles, proteins, viruses and - as I discovered, mRNAs.
>>

Treatment with tumor-treating fields (TTFields) suppresses intercellular tunneling nanotube formatio... Cancer-directed treatment using Tumor-Treating Fields spatially modulates the tumor microenvironment at the cellular and molecular levels, decreasing nanotube-based cellular networks of communication ...

Another cool-looking paper on #tunnelingnanotubes
🧪🔬
elifesciences.org/articles/853...

Hijacking intercellular trafficking for the spread of protein aggregates in neurodegenerative diseas... This review discusses TNTs's known and hypothesized biological relevance in vitro and in vivo in physiological and neurodegenerative contexts within the spectrum of intercellular communication mechani...

A nice short review on #tunnelingnanotubes by Chiara Zurzolo

www.oaepublish.com/articles/evc...
🧪🔬