Top: CD133 showed specific enrichment on the apical surface of radial glial cells (RGCs) in the developing ventricular zone (VZ) from postnatal day 0 (P0) to P15. Ciliary membrane marker (ARL13B) and the ciliary microtubule marker acetylated-tubulin (ac-TUB) indicated ependymal cell (EPC)-lineage differentiation. Cell proliferating marker (Ki67) and neural stem cell (NSC)-lineage marker (ASCL1) indicated NSC-lineage transition. The framed region was further magnified to show details. The boundary between lateral ventricle (LV) and VZ was denoted by the yellow dotted line for better distinguishing ciliary ac-TUB and cytoplasmic ac-TUB in (B). The scale bars are 20 μm. Bottom: A model illustrating how TFEB functions in the process of bGPC specification during VZ development.
#NeuralStemCells (NSCs) & ependymal cells (ECs) are derived from #RadialGlialCells. This study uses #scRNAseq to characterize cell fate trajectories in the developing #VentricularZone, identifying TFEB as a regulator of the NSC/EPC balance @plosbiology.org 🧪 plos.io/3HbrsJG