Photocaged Chloroquine Derivatives for the Light-Dependent Inhibition of Autophagy in Cancer Stem Cells Chloroquine (CQ) and hydroxychloroquine (HCQ) inhibit autophagy and have shown promise as adjuvant anticancer agents, particularly for targeting therapy-resistant cancer stem cells (CSCs). However, their clinical utility is limited by systemic toxicity and poor tumor selectivity. Here, we report the design, synthesis, and photochemical evaluation of [7-(diethylamino)coumarin-4-yl]methyl (DEACM)-caged CQ and HCQ derivatives as visible-light-activated autophagy inhibitors. Selective caging of the aliphatic amine suppressed biological activity in the dark and enabled rapid release of the parent drugs upon illumination. The lead compound 1C displayed robust light-dependent cytotoxicity across multiple cancer cell lines and, upon photoactivation, recapitulated CQ’s effects on LC3-II accumulation. In CSC-enriched tumorspheres, 1C completely abolished sphere formation only if illuminated. Ex vivo and in vivo studies confirmed that visible light penetrates tumor tissue sufficiently to activate 1C and locally release CQ within the tumor. These findings establish the first proof of concept for light-controlled autophagy inhibition and provide a blueprint for spatiotemporally confined anticancer therapies based on photopharmacological modulation of CSCs.

Photocaged Chloroquine Derivatives for the Light-Dependent Inhibition of Autophagy in Cancer Stem Cells pubs.acs.org/doi/10.1021/... #hvhebron #bio #cibbim #onco [Text complet] @pubs.acs.org

Naturally-Derived Cellulose-Chitosan Soft-Hydrogel for Sustained Local Docetaxel Micelles Delivery: From In Silico Modeling to Preclinical Validation in Ovarian and Glioblastoma Models Natural biopolymer-based hydrogels represent a promising strategy for localized drug delivery in cancer therapy, helping to overcome the systemic toxi…

Naturally-Derived Cellulose-Chitosan Soft-Hydrogel for Sustained Local Docetaxel Micelles Delivery: From In Silico Modeling to Preclinical Validation in Ovarian and Glioblastoma Models www.sciencedirect.com/science/arti... #hvhebron #bio #cibbim [Text complet]

Portable colorimetric absorbance reader with extended dynamic range via time-resolved sensing Many bioanalytical methods result in the generation of colorimetric readouts, with color intensity providing information of the concentration of the t…

Portable colorimetric absorbance reader with extended dynamic range via time-resolved sensing www.sciencedirect.com/science/arti... #hvhebron #cibbim

Amino Acid Residue-Driven Nanoparticle Targeting of Protein Cavities: Surface Chemistry Dictates Binding Specificity beyond Size Complementarity Cavities at protein-protein interaction interfaces are considered “undruggable” because their shallow or large geometries hinder stable binding by small molecules. Overcoming this limitation is essential for developing new therapies. Cerium oxide nanoparticles (CeO2NPs) of a suitable size can occupy the 5 nm central cavity of the SARS-CoV-2 spike (S) trimer, thereby inhibiting infection. Although size compatibility enables cavity access, other targeting parameters remain unclear. The S trimer features central and lateral cavities, both ∼5 nm in diameter, that can accommodate NPs. To explore factors governing cavity targeting, we compared size-matched CeO2NPs and gold NPs (AuNPs), evaluating their binding to S-trimer cavities and investigating mechanisms underlying their selective interactions. Despite comparable antiviral activity, the two NPs exhibited different binding profiles. Biolayer interferometry confirmed strong binding of both NPs to the S trimer, while only CeO2NPs selectively targeted the receptor-binding domain (RBD). CeO2NPs preferentially occupied the central cavity enriched with Asp residues. However, AuNPs bind to the lateral cavities, including the S1/S2 cleavage site, where they interact with Arg-rich motifs for furin-mediated activation of the S protein. Mechanistically, CeO2NPs achieved stable binding by coordinating with Asp carboxyl groups in the central cavity, while AuNPs bound lateral cavities primarily via electrostatic attraction with Arg residues. These findings suggest that residue-level recognition governs NP binding among sterically accessible protein cavities, with size compatibility enabling access and surface-residue matching defining binding selectivity. The insight provides a strategy for designing NPs to engage recessed features on protein surfaces previously inaccessible, enabling selective functional modulation and broader biomedical applications.

Amino Acid Residue-Driven Nanoparticle Targeting of Protein Cavities: Surface Chemistry Dictates Binding Specificity beyond Size Complementarity pubs.acs.org/doi/10.1021/... #hvhebron #cibbim

Trojan Horse Effect of Biologically Aged Microplastics-An Intracellular Carrier of LPS for Licensing Noncanonical Inflammasome Activation pubs.acs.org/doi/10.1021/... #hvhebron #cibbim

Additional file 1 of Insights into the causal role of diesel exhaust particles in ventricular arrhythmogenesis: protective effects of antioxidant cerium oxide nanoparticles Additional file 1.

Additional file 1 of Insights into the causal role of diesel exhaust particles in ventricular arrhythmogenesis: protective effects of antioxidant cerium oxide nanoparticles springernature.figshare.com/articles/jou... #hvhebron #cardio #cibbim

Additional file 2 of Insights into the causal role of diesel exhaust particles in ventricular arrhythmogenesis: protective effects of antioxidant cerium oxide nanoparticles Additional file 2.

Additional file 2 of Insights into the causal role of diesel exhaust particles in ventricular arrhythmogenesis: protective effects of antioxidant cerium oxide nanoparticles springernature.figshare.com/articles/jou... #hvhebron #cardio #cibbim

Response of Ocular Bacteria Exposure to Cerium Oxide Nanoparticles in Eye Drop Formulation - Current Microbiology Cerium oxide nanoparticles (CeO2 NPs), a few nanometers in diameter, exhibit strong antioxidant and anti-inflammatory activity and are considered promising candidates for the treatment of ocular disea...

Response of Ocular Bacteria Exposure to Cerium Oxide Nanoparticles in Eye Drop Formulation link.springer.com/article/10.1... #hvhebron #oft #cibbim

Size Control of Highly Monodisperse Citrate-Stabilized Magnetite Nanoparticles in Aqueous Media: The Role of Cerium Cations Controlling the nucleation and growth of magnetite nanoparticles (NPs) via aqueous coprecipitation at room temperature is inherently challenging due to kinetic and thermodynamic constraints. Rapid pH ...

Size Control of Highly Monodisperse Citrate-Stabilized Magnetite Nanoparticles in Aqueous Media: The Role of Cerium Cations pubs.acs.org/doi/full/10.... #hvhebron #cibbim [Text complet]

Scavenging Reactive Oxygen Species by Cerium Oxide Nanoparticles Prevents Death in a Peripheral T Cell Lymphoma Preclinical Mouse Model Cancer cell survival and proliferation are correlated with increased metabolic activity and consequent oxidative stress, driving metabolic shifts that interfere with the immune response to malignant c...

Scavenging Reactive Oxygen Species by Cerium Oxide Nanoparticles Prevents Death in a Peripheral T Cell Lymphoma Preclinical Mouse Model pubs.acs.org/doi/10.1021/... #hvhebron #cibbim

Relevance of Sentinel Nodes in Atypical Territories in Surgically Treated Patients With T1‐2N0 Oral Squamous Cell Carcinoma Objectives To assess the usefulness of sentinel lymph node biopsy (SLNB) in patients with early-stage oral squamous cell carcinoma (OSCC). Materials and Methods Seventy-five patients (mean age 62 ...

Relevance of Sentinel Nodes in Atypical Territories in Surgically Treated Patients With T1-2N0 Oral Squamous Cell Carcinoma onlinelibrary.wiley.com/doi/10.1002/... #hvhebron #com #mnucl #cibbim

Silver Nanoparticles and Antibiotics: A Promising Synergistic Approach to Multidrug-Resistant Infections www.mdpi.com/2076-2607/13... #hvhebron #cibbim [Text complet]

Pericardium decellularization in a one-day, two-step protocol - Molecular and Cellular Biochemistry Scaffolds used in tissue engineering can be obtained from synthetic or natural materials, always focusing the effort on mimicking the extracellular matrix of human native tissue. In this study, a dece...

Pericardium decellularization in a one-day, two-step protocol link.springer.com/article/10.1... #hvhebron #cibbim [Text complet]

Metallic nanoparticles biodistribution for the study of lymphoma in animal models T cell lymphoma constitutes a complex group of diseases, characterized by heterogeneous molecular features and clinical symptoms, and a dismal outcome…

Metallic nanoparticles biodistribution for the study of lymphoma in animal models www.sciencedirect.com/science/arti... #hvhebron #cibbim

Preclinical Development of Magnetic Nanoparticles for Hyperthermia Treatment of Pancreatic Cancer Pancreatic ductal adenocarcinoma (PDAC) is a very challenging disease with a very poor prognosis. It is characterized by a dense desmoplastic stroma that hampers drug penetration and limits the effectiveness of conventional chemotherapy (CT). As an alternative, the combination of CT with hyperthermia (HT) has been proposed as an innovative treatment modality for PDAC. In previous works, we reported on the development of iron oxide magnetic nanoparticles (MNPs) that, when exposed to time-varying magnetic fields, exhibit strong HT responses that inhibited the growth of pancreatic cancers. We report here on advances toward the clinical use of these MNPs as an intratumorally administered sterile magnetic fluid (the “NoCanTher ThermoTherapy” or “NTT” Agent) alongside intravenous standard-of-care drugs (gemcitabine and nab-paclitaxel) for the treatment of PDAC. In vitro cell viability assays show that the combination of low doses of CT and HT is highly synergistic, particularly in the BxPC-3 cell line. In vivo, biodistribution assays showed that the NTT Agent MNPs remained mainly within the tumor, concentrated around areas with a high stromal component. Moreover, the combined CT/HT treatment shows clear advantages over CT alone in terms of drug penetration and reduction of the tumor volume, suggesting a potential direct effect of HT in the disruption of the interstitial stroma to facilitate the access of the drugs to malignant cells. These studies have led to the approval and commencement of a clinical investigational study at the Vall d’Hebron University Hospital (Barcelona, Spain) of the NTT Agent alongside CT in patients with locally advanced PDAC.

Preclinical Development of Magnetic Nanoparticles for Hyperthermia Treatment of Pancreatic Cancer pubs.acs.org/doi/10.1021/... #hvhebron #cibbim

Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe3+/Fe2+) of Prussian blue (PB)...

Peroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles www.mdpi.com/2079-4991/15... #hvhebron #cibbim [Text complet]

Hazard assessment of nanomaterials: how to meet the requirements for (next generation) risk assessment - Particle and Fibre Toxicology Background Hazard and risk assessment of nanomaterials (NMs) face challenges due to, among others, the numerous existing nanoforms, discordant data and conflicting results found in the literature, and...

equirements for (next generation) risk assessment particleandfibretoxicology.biomedcentral.com/articles/10.... #hvhebron #cibbim [Text complet]