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In this thread we will explain you how we put together
#SPT #SMLM #cryoET #cryoCLEM + #modeling
to learn about the higher-order mechanism that controls exocytosis (constitutive exocytosis, the one😜)
(Illustrative animation made by the amazing Dylan Godfrey)
Valuable advance in correlative #cryoCLEM - #CryoET imaging - especially applied to a challenging plant tissue!
Detailed workflow and beautiful ultrastructure details of plant cell and root barriers! ❄️🔬🌱
Must read for all cryoimaging enthusiasts!
#plantscience #microscopy #cryoimaging
This study was key to establishing #cryoCLEM and #cryoET in Bordeaux.
It is an important milestone.
@davidp-bdx.bsky.social,
Étienne Herzog and @paulosh.bsky.social took the risk to be the first to use this approach with us.
🙏🙏🙏
Sincere congratulations to our colleague Paul Lapios @paulosh.bsky.social on his thesis defence this morning.
I'm very pleased to see that all the efforts made to get the #CryoCLEM and #CryoET workflows up and running in Bordeaux have paid off and are producing meaningful results.
#IINSPhD🧑🎓 @paulosh.bsky.social, PhD student in David Perrais' team, #MemTraS, defends his thesis: “Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses”
He worked on the #synapse, #dopamine, #striatum and #cryoCLEM
www.bordeaux-neurocampus.fr/event/souten...
All of the cryo workshops today @IMBatUQ on location for coffee. #cryoCLEM with @AriottiNick and this cute little guy with the symmetrical decals. #entomology and #tomography unite 😃 #whatbug ??
#ImageOfTheMonth Fluorescence cool as ice! Mammalian cells were labeled with fluorescent dyes & then vitrified, ready for #CryoCLEM This is the first step towards in cellulo structures with sub-nanometer precision in #cryoET @CFcryo 👏Congrats to @daniel_roderer @BroichhagenJ
