Recent Advances in CRISPR-Cas Systems for Dengue Virus Detection

dl.begellhouse.com/journals/6db...

#CRISPRDiagnostics #DengueVirus #MolecularDiagnostics #CRE

ECs natural sites of infection for several viruses, incl human #cytomegalovirus (HCMV), #SARSCoV2, #denguevirus, #hantavirus, #Ebolavirus, #Nipahvirus, Colorado tick fever virus & others. Their strategic location at the interface between the bloodstream & tissue makes them critical

Discovery of a Novel Non‐Nucleoside Inhibitor of RNA‐Dependent RNA Polymerase Against Dengue Virus PN-1, a novel non-nucleoside inhibitor of the dengue virus RNA-dependent RNA polymerase (RdRp), was identified from Panax notoginseng. This compound selectively targets the DENV-2 NS5 RdRp, inducing ....

#Article in #MedComm
Discovery of a Novel Non-Nucleoside Inhibitor of RNA-Dependent RNA Polymerase Against Dengue Virus doi.org/10.1002/mco2...

#AG129mice #DengueVirus #DENV #ICRsuckling #Target #PN_1

September Fellow's Spotlight on Dr. David Martinez Our September Fellows Spotlight goes to Keystone Symposia Fellow Dr. David Martinez of Yale Medical School who played a critical role in research and development of the COVID-19 vaccines and antibody ...

ICYMI: Our Sept Fellows Spotlight features Dr. David Martinez @davidmartinez.bsky.social (@yaleschoolofmed.bsky.social) and his work on #InfectiousDisease, #DengueVirus & #COVIDVaccines. Learn more + apply to be a #KSFellow! 🔗 keypoint.keystonesymposia.org/home/septemb...

#KeystoneSymposia

A retrospective investigation for the seroprevalence of Chikungunya virus and its co-existence with Dengue virus in Pakistani population, 2014-2015 Our study falls between 2014 and 2015, proficiently covering the whole period of illness including early onset of infection till the convalescent period for both Dengue and Chikungunya infection. This study was first of its type for specific period that...

A retrospective investigation for the seroprevalence of Chikungunya virus and its co-existence with Dengue virus in Pakistani population, 2014-2015
->Nature | #DengueVirus | More from BigEarthData.ai

Glycans on non-structural protein 1 prevent premature #Tcell mediated #DengueVirus clearance

By F. Idris, S. Alonso & colleagues at National Centre for Infectious Diseases Singapore

🗞️ doi.org/10.1038/s443...

How billions of hacked mosquitoes and a vaccine could beat the deadly dengue virus Support for this article was provided by the Pulitzer Center. Last month, a parade of vehicles wound its way through three cities in Brazil, releasing clouds of mosquitoes into the air. The insects all carry a secret weapon — a...

How billions of hacked mosquitoes and a vaccine could beat the deadly dengue virus
->Nature | #Health #DengueVirus #Vaccine | More info from BigEarthData.ai

Pre-existing YFV-17D immunity mediates T cell cross-protection against dengue virus serotype 2 infection in mice We have complied with all relevant ethical regulations for animal use. All experimental procedures involving animals were conducted in accordance with institutional guidance and approved by the Rutgers, The State University of New Jersey Institutional Animal Care and Use Committee...

Pre-existing YFV-17D immunity mediates T cell cross-protection against dengue virus serotype 2 infection in mice
->Nature | #Infection #DengueVirus | More info from EcoSearch

Hawaiʻi DOH reports 12th travel-related dengue virus case of 2025 Aedes albopictus is a widespread invasive mosquito in Hawaiʻi. Photo Credit: James Gathany, CDC. The Hawaiʻi Department of Health has confirmed a new travel-related case of dengue virus on Oʻahu, bringing the total number of dengue cases to 12 in...

Hawaiʻi DOH reports 12th travel-related dengue virus case of 2025
->Maui Now | #DengueVirus | More info from EcoSearch

Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants Human blood samples to prepare mosquito artificial infectious bloodmeals were supplied by healthy adult volunteers at the ICAReB biobanking platform (BB-0033-00062/ICAReB platform/Institut Pasteur, Paris/BBMRI AO203/[BIORESOURCE]) of the Institut Pasteur in the CoSImmGen and Diagmicoll protocols, which had been approved by...

Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants
->Nature | #DengueVirus | More info from EcoSearch

DOH reports 10th travel-related dengue virus case of 2025 Aedes albopictus is a widespread invasive mosquito in Hawai’i. Photo Credit: James Gathany, CDC. The Hawai‘i Department of Health has confirmed a new travel-related case of dengue virus on Oʻahu, bringing the total number of dengue cases to 10 in...

DOH reports 10th travel-related dengue virus case of 2025
->Maui Now | #DengueVirus | More info from EcoSearch

Comparative analysis of phytocompounds and repurposed drugs against dengue virus serotypes employing an in silico study The screening process was meticulously conducted following an extensive literature search to identify 93 phytocompounds and 15 drugs with documented potential for antiviral activity. These compounds were subjected to rigorous screening using DataWarrior based on predefined criteria encompassing drug likeness,...

Comparative analysis of phytocompounds and repurposed drugs against dengue virus serotypes employing an in silico study
->Nature | #DengueVirus | More info from EcoSearch

Implications of successive blood feeding on Wolbachia -mediated dengue virus inhibition in Aedes aegypti mosquitoes Ae. aegypti mosquito lines (WT, wMelM, and wAlbB) were generated from natively uninfected Ae. aegypti collected near Cairns, Queensland, Australia, that were kept uninfected (WT; wildtype mosquitoes without Wolbachia) or transinfected with either Wolbachia wAlbB or wMelM strain via microinjection35,36...

Implications of successive blood feeding on Wolbachia -mediated dengue virus inhibition in Aedes aegypti mosquitoes
->Nature | #DengueVirus | More info from EcoSearch

Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells Human megakaryocytes in vitro cell line (MEG-01 cells derived from bone marrow of a leukemic patient, catalog number CRL-2021) or human endothelial cells (EA.hy926, catalog number CRL-2922) were obtained from American Type Culture Collection (ATCC). MEG-01 or EA.hy926 cells were...

Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells
->Nature | #DengueVirus | More info from EcoSearch

Clinico-genomic study reveals association of dengue virus genome high frequency mutations with dengue disease severity RNA isolated from the 1310 serum samples collected from NS1-antigen-confirmed dengue patients was used for serotype-specific qRT-PCR (Fig. 1A). Out of 1310, 153 were found positive for only DENV-2 (Table 3). Additionally, n = 3 and n = 2 were...

Clinico-genomic study reveals association of dengue virus genome high frequency mutations with dengue disease severity
->Nature | #Disease #DengueVirus #Health | More info from EcoSearch

#Medsky🧪 #IDsky #Immunosky #publichealth #Denguevirus is the most abundant and rapidly spreading arbovirus worldwide. 390 mil dengue infections are estimated to occur annually, of which approximately 25% are symptomatic.
Climate change and urbanisation have

🍃 One of the most widespread #arboviral #diseases in the world, #denguevirus #disease is primarily found in tropical and subtropical regions, affecting 129 countries, however, there is currently no approved #antiviral #medication for this condition.

💻 www.thieme-connect.de/products/ejo...

DOH reports eighth travel-related dengue virus case of 2025 Aedes albopictus is a widespread invasive mosquito in Hawai’i. Photo Credit: James Gathany, CDC. The Hawai‘i Department of Health has confirmed a new travel-related case of dengue virus on Oʻahu, bringing the total number of dengue cases to eight in...

DOH reports eighth travel-related dengue virus case of 2025
->Maui Now | #DengueVirus | More info from EcoSearch

Seven dengue cases in Hawaii prompt travel warnings for Oahu visitors A new travel-related dengue case has been confirmed on Oahu, raising the state’s total to seven this year.

#Dengue incidences in #Hawaii prompt #travel warnings ...

| #denguevirus | #infections | #Oahu | #publichealth | Via kitv .com

Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB Establishment of Dhaka Ae. aegypti colony During June 2019, mosquito eggs were collected from five areas of Dhaka, Bangladesh using oviposition traps and shipped to QIMR Berghofer (QIMRB), Australia (import permit: 0002569896). Collections from one of the sites were not...

Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB
->Nature | #DengueVirus | More info from EcoSearch

Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA) The first 70 nucleotides corresponding to SLA from the dengue virus serotype 2 genome (New Guinea C strain, GenBank no AF038403.1) was synthesized by GenScript in non-biotin and biotin-tagged forms. Lyophilized RNA samples were dissolved in respective buffers used for...

Structural dynamics of the dengue virus non-structural 5 (NS5) interactions with promoter stem-loop A (SLA)
->Nature | #DengueVirus | More info from EcoSearch

Repurposing FDA-approved drugs and natural compounds to inhibit the RNA-dependent RNA polymerase domain of dengue virus 2 or dengue virus 3 To determine the key residues involved in the binding site, crystal structures of the complexes (PDB ID: 6IZZ and PDB ID: 6IZX) were analyzed using Discovery Studio. The amino acids that interact with RdRp of DENV-3 include residues S763, R773,...

Repurposing FDA-approved drugs and natural compounds to inhibit the RNA-dependent RNA polymerase domain of dengue virus 2 or dengue virus 3
->Nature | #DengueVirus | More info from EcoSearch

Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection The human pathogenic viruses in the genus Orthoflavivirus are primarily transmitted to humans by hematophagous arthropods such as ticks and mosquitoes40. In tropical and sub-tropical countries around the world, the primary concern is those viruses that are transmitted by mosquitoes,...

Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection
->Nature | #DengueVirus #VirusInfection | More info from EcoSearch

Zika but not Dengue virus infection limits NF-κB activity in human monocyte-derived dendritic cells and suppresses their ability to activate T cells Human blood was collected from healthy donors (negative for human immunodeficiency virus, hepatitis B, and hepatitis C) under Protocol VD-057-0217 from the La Jolla Institute for Immunology and Protocol 181624 from UC San Diego. All recruited volunteers provided written informed...

Zika but not Dengue virus infection limits NF-κB activity in human monocyte-derived dendritic cells and suppresses their ability to activate T cells
->Nature | #VirusInfection #DengueVirus | More info from EcoSearch

CDC has issued a Health Alert Network (HAN) Health Update to provide additional information about the ongoing risk of #denguevirus (DENV) infections and updates to testing recommendations in the United States: www.cdc.gov/han/2025/han...

Hijacking of plasmin by dengue virus for infection

Hijacking of plasmin by dengue virus for infection
->ScienceDaily | #Infection #DengueVirus | More info from EcoSearch

#MeetTheLab

This week, we are happy to introduce the Zitzmann Lab from @oxfordbiochemistry.bsky.social

The lab focuses on developing antiviral therapies to address #HepatitisB ( #HBV), #HepatitisC ( #HCV), human immunodeficiency virus ( #HIV), #DengueVirus ( #DENV), #Ebola, and #influenza.

Genetic and structural characterization of dengue virus involved in the 2023 autochthonous outbreaks in central Italy Dengue virus (DENV) has been expanding its range to temperate areas that are not usually affected, where the spread of vectors has been facilitated by global trade and climate change. In Europe, th...

#GenomicSurveillance of #DengueVirus in Italy reveals local transmission and viral adaptations linked to human and vector interactions.
#Arbovirus #VirusEvolution #ClimateChange

📄 doi.org/10.1080/2222...
EVBC👤: Cesare Gruber

Texas reports first locally acquired case of dengue virus in 2024 - KPRC FILE - This 2003 photo provided by the Centers for Disease Control and Prevention shows a female Aedes albopictus mosquito acquiring a blood meal from a human host. Dengue, a tropical illness caused by a virus, is spread by Aedes...

Texas reports first locally acquired case of dengue virus in 2024 - KPRC
->Click2Houston | #DengueVirus | More info from EcoSearch

Texas officials announce first locally acquired case of dengue virus in 2024 There have been 106 travel-associated dengue cases in Texas this year. Texas public health officials announced on Monday the first locally acquired case of dengue virus of the year was reported in a Cameron County resident. There have been 106...

Texas officials announce first locally acquired case of dengue virus in 2024
->ABC News | #DengueVirus | More info from EcoSearch