Peeling back the layers. Study finds #pseudoexons as a mechanism via which deep #intronic #variants cause #Mendeliandisease bit.ly/46VDPT7

Transcriptional regulation of Ligase IV by an intronic regulatory element directs thymocyte development - Genes & Immunity Genes & Immunity - Transcriptional regulation of Ligase IV by an intronic regulatory element directs thymocyte development

#Transcriptional #regulation of #Ligase IV by an #intronic #regulatory #element directs #thymocyte #development

#Genes & #Immunity @springernature.com
www.nature.com/articles/s41...

So we encourage to plot boxplots for scRNAseq qcing for #nUMI, #mito, #MALAT1 and #intronic content or a combination of them like Figure 6 in our publication!

Keep an eye to your sc dataset, 1)are there two clusters of same cell type one with and the other without #MALAT1 expression?
2)extreme values of #MALAT1 or #intronic reads is bad, null values indicate citosolic debris and extreme high values bare nuclei (dying/damaged cell)