#mdpimolecules

A Potent Quinone Reductase Encoded by ywqN (Qnr1) Protects Bacillus subtilis from Oxygen Radical Genotoxicity www.mdpi.com/3733756 #mdpimolecules via @Molecules_MDPI

I’m excited to share this work that has been a long time coming that validates network approaches with functional assays can guide the isolation of bioactive compounds. 1H-NMR Guided Isolation of Bioactive Compounds from Species of the Genus Piper www.mdpi.com/3294860 #mdpimolecules

Simple Enzyme Immobilization for Flow Chemistry? An Assessment of Available Strategies for an Acetaldehyde-Dependent Aldolase Enzyme immobilization is a technology that enables (bio-)catalysts to be applied in continuous-flow systems. However, there is a plethora of immobilization methods available with individual advantages and disadvantages. Here, we assessed the influence of simple and readily available methods with respect to the performance of 2-deoxy-d-ribose-5-phosphate aldolase (DERA) in continuous-flow conditions. The investigated immobilization strategies cover the unspecific attachment to carriers via epoxides, affinity-based attachment via metal ion affinity, StrepTag™-StrepTactin™ interaction as well as the covalent affinity attachment of an enzyme to a matrix tethered by the HaloTag®. The metal-ion-affinity-based approach outperformed the other methods in terms of immobilized activity and stability under applied conditions. As most enzymes examined today already have a HisTag for purification purposes, effective immobilization may be applied, as simple as a standard purification, if needed.

Another fresh publication :)

Simple Enzyme Immobilization for Flow Chemistry? An Assessment of Available Strategies for an Acetaldehyde-Dependent Aldolase https://www.mdpi.com/1862730 #mdpimolecules via @Molecules_MDPI

Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation Environmental pressures caused by population growth and consumerism require the development of resource recovery from waste, hence a circular economy approach. The production of chemicals and fuels from organic waste using mixed microbial cultures (MMC) has become promising. MMC use the synergy of bio-catalytic activities from different microorganisms to transform complex organic feedstock, such as by-products from food production and food waste. In the absence of oxygen, the feedstock can be converted into biogas through the established anaerobic digestion (AD) approach. The potential of MMC has shifted to production of intermediate AD compounds as precursors for renewable chemicals. A particular set of anaerobic pathways in MMC fermentation, known as chain elongation, can occur under specific conditions producing medium chain carboxylic acids (MCCAs) with higher value than biogas and broader applicability. This review introduces the chain elongation pathway and other bio-reactions occurring during MMC fermentation. We present an overview of the complex feedstocks used, and pinpoint the main operational parameters for MCCAs production such as temperature, pH, loading rates, inoculum, head space composition, and reactor design. The review evaluates the key findings of MCCA production using MMC, and concludes by identifying critical research targets to drive forward this promising technology as a valorisation method for complex organic waste.

New review paper out on valorisation of food waste via fermentation by @VickyTheGroove et al. from @csctbath @WIRCBath @UniofBath
#mdpimolecules Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation@Molecules_MDPI

mdpi.com/399992