🧬 S-SELeCT: #phiC31 evolved in human cells for endogenous site A (chr4p14, symmetrical pseudo-attP)

Stepwise engineering (A1-A5 modules) + dMad7 tethering (g10/g6 #gRNAs) → V12-NL1a4-dMad7

10.2 kb donor integration:
Stable: 32.4%
Transient: 13.3% (mPGK #promoter, optimized density)