🧬 S-SELeCT: #phiC31 evolved in human cells for endogenous site A (chr4p14, symmetrical pseudo-attP)
Stepwise engineering (A1-A5 modules) + dMad7 tethering (g10/g6 #gRNAs) → V12-NL1a4-dMad7
10.2 kb donor integration:
Stable: 32.4%
Transient: 13.3% (mPGK #promoter, optimized density)
Not all gRNAs are created equally. Our rigorous QC methods show our #gRNAs are about 10% purer than the competition. Misleading data can skew your research—see the numbers for yourself and be confident when editing in sensitive applications: https://idtb.io/kx8kum
#CRISPR #gRNA #guideRNA
Our competitor claims to have high purity #gRNAs—but every guide looks pure if you don’t QC long enough. IDT’s HPLC gRNAs undergo a rigorous high-fidelity UHPLC QC and routinely achieve an ~10% higher purity rate than our competitor’s HPLC gRNAs. See the data: https://idtb.io/kx8kum
A transient transformation method for pre-screening #gRNAs in #CRISPR/Cas #gene editing
🌱https://doi.org/10.1016/j.jia.2025.04.007
✅Prof. Xiaoyang Ge team from CAAS
#GeneEditing #PlantScience
Some providers believe a 5-minute QC is enough to test guide RNAs’ purity—we don’t. That’s why all IDT HPLC #gRNAs are QC’d for >30 minutes and achieve on average a 10% higher purity rate than our competitor. Point, click, edit with confidence—guaranteed. See the data: https://idtb.io/bufao3
