With Sven zur Oven-Krockhaus, @luiselotterausch.bsky.social & Klaus Harter we wrote an #ExpertView on #sptPALM in #plants 🌱 (focus: #plasmamembrane).
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!

Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇

Excited to share our #bioRxiv #preprint on HIR2, RLKs and membrane organisation in #Arabidopsis. We used many exciting techniques like #sptPALM, #AlphaFold and #BN-PAGE 🌱

Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the applica...

Presenting our research to the Bsky Community – Part II! 🌱🔬

Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉

👉 Read more: tinyurl.com/2vcry74m

Four different figures showcasing strip plots, scatter plots, and box plots of synthetic #sptPALM data

#Python, #Seaborn & #Pandas is growing on me. Some synthetic data to plot average diffusion coefficients etc of tracks in individual bacterial cells (independent repeats of different conditions). #sptPALM is hard. Getting there @jmakela.bsky.social + @loreoliv.bsky.social

The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs

This week will hopefully bring the answer to the very important question whether mMaple3 is more photostable than PAmCherry2 in E. coli #sptPALM. Can't wait. @loreoliv.bsky.social

Fast dynamics in #sptPALM? Log-scale to the rescue. Three state model for Cas9 diffusion in <E. coli>.

graphical user interface for a simulation

nice plots

Playing with a GUI for #sptPALM simulations and #Python #Seaborn

Reading of a turbidostat showing stability over 100 hours.

8+ turbidostats in a row.

Some colleagues bought some #pioreactors for our next set of #sptPALM experiments. Stable for 100+ hours and you can exchange as much buffer as you like after reaching threshold (saw tooth). Excellent value for money it looks like.

The Escherichia coli replication initiator DnaA is titrated on the chromosome DNA replication initiation is orchestrated in many prokaryotes by the replication initiator DnaA. Two models for regulation of DnaA activity in Escherichia coli have been proposed: the switch between ...

New preprint spear headed by the amazing #LorenzoOlivi: "The Escherichia coli replication initiator DnaA is titrated on the chromosome" using, among many other techniques, #sptPALM to determine growth dependent mobilities of DnaA diffusing in live cells. doi.org/10.1101/2024.10.07.617004 1/n