Comprehensive new book by Mann et al.:

‘Cutting Edge Technologies for Developing Future Crop Plants’

Fig.12.2

tinyurl.com/y9jha5vt

#WIS

#PlantandEnvironmentalScience

#ReactiveOxygenSpecies

#ROS

#Oxidation

#PhotosystemII

#PSII

#PlasmaMembrane

#Apoplast

#CellWall

#NADPH

#Research by Deng-Ke Wang, Fraser J. Moss & Walter F. Boron examined the synergistic roles of aquaporin 5 and intra- and extracellular carbonic anhydrases in promoting #CO2 diffusion across the Xenopus oocyte #plasmamembrane 🔬

📜 Read the study: physoc.onlinelibrary.wiley.com/doi/10.1113/...

Client Challenge

This week #JournalClub @labsoap.bsky.social @nature.com #celldeath #plasmamembrane #rupture #MLKL #necroptosis www.nature.com/articles/s41...

With Sven zur Oven-Krockhaus, @luiselotterausch.bsky.social & Klaus Harter we wrote an #ExpertView on #sptPALM in #plants 🌱 (focus: #plasmamembrane).
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!

Formation and dynamics of the plasma membrane reservoir (PMR). The PMR forms prior to the development of the daughter cells’ IMC. Its position and size vary during replication, and it is reabsorbed at the conclusion of daughter cell budding. We propose that the PMR facilitates the expansion of the PM surface during replication. All scale bars represent 1 µm. While the PMR is not always visible due to its transient nature, virtually all the parasites undergoing replication are forming a PMR.

How do #apicomplexans reorganize their #PlasmaMembrane (PM) while replicating inside host cells? This study shows that #Toxoplasma recycles its PM via Rab5b- & MyoF-dependent #endocytosis, linking PM reservoir formation to membrane homeostasis via the micropore @plosbiology.org 🧪 plos.io/46tDxny

Formation and dynamics of the plasma membrane reservoir (PMR). The PMR forms prior to the development of the daughter cells’ IMC. Its position and size vary during replication, and it is reabsorbed at the conclusion of daughter cell budding. We propose that the PMR facilitates the expansion of the PM surface during replication. All scale bars represent 1 µm. While the PMR is not always visible due to its transient nature, virtually all the parasites undergoing replication are forming a PMR.

How do #apicomplexans reorganize their #PlasmaMembrane (PM) while replicating inside host cells? This study shows that #Toxoplasma recycles its PM via Rab5b- & MyoF-dependent #endocytosis, linking PM reservoir formation to membrane homeostasis via the micropore @plosbiology.org 🧪 plos.io/46tDxny

Formation and dynamics of the plasma membrane reservoir (PMR). The PMR forms prior to the development of the daughter cells’ IMC. Its position and size vary during replication, and it is reabsorbed at the conclusion of daughter cell budding. We propose that the PMR facilitates the expansion of the PM surface during replication. All scale bars represent 1 µm. While the PMR is not always visible due to its transient nature, virtually all the parasites undergoing replication are forming a PMR.

How do #apicomplexans reorganize their #PlasmaMembrane (PM) while replicating inside host cells? This study shows that #Toxoplasma recycles its PM via Rab5b- & MyoF-dependent #endocytosis, linking PM reservoir formation to membrane homeostasis via the micropore @plosbiology.org 🧪 plos.io/46tDxny

Revealing Plasma Membrane Protein Dynamics in Living Plant Cells with Single-Molecule Tracking Abstract. The behavior of proteins and other biomolecules in cellular environments is governed by complex molecular processes. Understanding their temporal

Check out our latest review on investigating plant plasma membrane proteins with super resolution microscopy 🔬 🌱 in @jxbotany.bsky.social

#superresolution #plasmamembrane

academic.oup.com/jxb/advance-...

Lipid-Protein Interplay: How the Plant Plasma Membrane H-ATPase Dynamically Interacts with Its Lipid Environment - A Review - Premier Science Keywords: H+-atpase regulation, Lipid-protein interactions, Plant plasma membrane, Sterol influence, Sphingolipid role.

doi.org/10.70389/PJP...

#lipid #protein #plant #plasmamembrane #atpase

Thank you Matteo Bonazzi @irim-life.bsky.social
#infectiousdiseases #inflammation #cellbiology #autophagy #endocytosis #plasmamembrane #celladhesion

Lysosomal #LAMP1 in red, #cytoskeleton #tubulin in cyan, nuclear #DNA staining in blue and the #PlasmaMembrane in gray.
While each cellular #organelle has specialized roles in the cell, their activities are well coordinated and are under a tight cross-regulation.

Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...

#NewNProt: Measuring #plasmamembrane fluidity using #confocalmicroscopy bit.ly/4bie557 #membranefluidity

We have a #SuperResolution #SMLM image of #mitochondria (magenta) and #lysosomes (yellow), and a diffraction-limited image of the #nucleus (blue). Next, we have the #cytoskeleton in green, alone or with the #PlasmaMembrane in gray. All layers can be generated with our user-friendly software, CODI.

Fixed Cos7 cells were fixed and stained in 5 colors:
🔬 #SMLM – The mitochondrial protein #Tomm20 (yellow) and the lysosomal marker #LAMP11 (red).
🔬 In diffraction-limited imaging – #Tubulin (cyan), the nuclear marker Hoechst (#DNA, magenta), and the #PlasmaMembrane dye CellMask™ Green (gray).

In addition, we have 3 diffraction-limited channels that provide a wider cellular context, namely, the #PlasmaMembrane, #Nucleus and the #microtubule #cytoskeleton, all used to facilitate accurate cellular orientation.

👋🏻Our November issue's out!
Cover: Imaging #Ovulation

👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...

Sub-membrane actin rings compartmentalize the plasma membrane There is mounting evidence that the cytoskeleton organizes the plasma membrane. Due to the dynamic nature of cytoskeletal proteins, direct experimental confirma

Very excited to share my PhD work with @ewerslab.bsky.social at @freieuniversitaet.bsky.social out at JCB! It's all about actin rings and how they compartmentalize the #plasmamembrane! A short thread 1/5

rupress.org/jcb/article-...