🦷 A new study by @marinemorvan.bsky.social
High-throughput paleoproteomics method using MALDI-CASI-FTICR mass spectrometry to estimate biological sex from tooth enamel.
Great success on 130 individuals from medieval Great Moravia.
#Bioarchaeology #Archaeology #Proteomics #TooMS 😉 #Teammasspec
Proteins connect life at every level — and XL-MS is showing how.
Join researcher Lan Huang today at 12:15 p.m. Eastern for an #ASBMBBreakthroughs webinar to explore how this tech maps protein networks in stunning detail.
☑️ Free registration: www.asbmb.org/meetin....
#TeamMasSpec
Caroline in a patterned dress presents to an audience in a tiered lecture theatre with curved wooden seating. The projection screen shows a colorful diagram with molecular structures and text about "MATERIALS (ID, Modification)" including bullet points about understanding artist intention, artwork changes, treatment effects, and societal context. Attendees sit on wooden benches taking notes, with laptops open throughout the amphitheatre.
🧵 Thread: 1️⃣/ Caroline Tokarski delivering a masterclass on how #massspec revolutionises our understanding of cultural materials! Her slide perfectly encapsulates the multifaceted insights we can extract - from artist intention to societal context. #teammasspec #heritage
A packed amphitheatre lecture hall with curved wooden tiered seating filled with attendees listening to a presentation. The projection screen displays a split image comparing modern mass spectrometry equipment on the left with Rembrandt's famous 1632 painting "The Anatomy Lesson of Dr. Nicolaes Tulp" on the right, branded with "RIJKS MUSEUM". The historic venue features elegant wooden architecture with multiple levels of curved benches rising steeply upward.
Alba Alvarez Martin from @rijksmuseum.bsky.social
presenting "Making Mass Spectrometry Accessible in Cultural Institutions".
The historical irony isn't lost as Rembrandt's anatomy lesson is juxtaposed with #teammasspec in an amphitheatre designed for anatomical dissection.
Bird's eye view of a circular lecture theatre with curved wooden tiered seating arranged in concentric arcs. At the bottom, a projection screen displays "Advanced mass spectrometry applied to cultural heritage Symposium" with University of Bordeaux branding. Two people stand near the presentation area at the front, with wooden chairs and desks arranged in the curved amphitheatre design typical of academic venues.
A woman with glasses and light blue striped shirt sits at a curved wooden desk in a tiered lecture theatre. The amphitheatre features multiple levels of curved wooden benches rising upward, with another person visible in the distant upper seating. The architectural design shows elegant curved lines and wood paneling throughout the academic venue.
Alternative perspectives at the "Advanced Mass Spectrometry in Cultural Heritage" symposium in Bordeaux!
www.u-bordeaux.fr/evenements/s...
The 3rd of these essential gathering brings together experts from across the globe to explore cutting-edge applications in heritage science. #teammasspec
I have a classic: “Have you confirmed your MS results by Western blots?” #massspec #teammasspec #proteomics #academicsky
6️⃣ This approach builds on solid foundations from food science - while offering new applications in archaeology. It has legs.
NB: @helenfewlass.bsky.social, you will soon have your NIR model for bone collagen estimation out... right? 👍
#teammasspec #archaeology #bioarchaeology
Diagram illustrating sex determination using amelogenin proteins in tooth enamel. The top shows X and Y chromosomally encoded protein isoforms (AMELX in blue, AMELY in red) with their amino acid sequences highlighted. The middle section displays amelogenin's structural progression from protein to nanospheres to matrix to enamel rods, alongside images of developing tooth structure with labeled components (ameloblasts, enamel, dentin, odontoblasts, pulp). The bottom shows Raman spectroscopy setup with IR laser and detector analyzing a tooth, demonstrating how incident light produces both Rayleigh scattering and Raman scattering from molecular vibrations, resulting in sex-specific spectra shown in a graph (blue and red lines) with male and female symbols. Biological sex determination is essential for analyzing human skeletal remains in archaeology, anthropology, and forensic science. This study investigates whether Raman spectroscopy of intact human dental enamel can be used as a non-destructive method for sex estimation. Orthogonal partial least squares discriminant analysis (OPLS-DA) and logistic regression identified sex-specific spectral characteristics in 88 human teeth from 47 modern individuals (26 females, 21 males). The OPLS-DA model showed excellent performance, with R2Y(cum) = 0.943 and Q2Y(cum) = 0.895. Raman shift wavenumbers at 373, 1182, and 1600 cm−1 were identified as the most reliable discriminators and included in a final logistic regression model. This model achieved an area under the receiver operating characteristic curve (ROC-AUC) of 0.98, a sensitivity of 0.87, and a specificity of 0.94. Our results indicate that Raman spectroscopy can effectively differentiate male and female human dental enamel based on subtle differences in molecular composition—possibly linked to residual differences between AMELX and AMELY-derived proteins.
🧵 Thread: 1️⃣ Fascinating paper by Stewart et al. shows robust method for sex determination using peptides in tooth enamel.
Great potential for #archaeology with fragmentary remains where DNA is degraded. #palaeoproteomics #ZooMS #teammasspec
figshare.com/s/a861c0d74d...
2/9 The authors present two novel algorithms: isoBLAST and ClassiCOL, which embrace peptide ambiguity rather than trying to eliminate it - a conceptual shift in palaeoproteomics. #teammasspec
3/5 🔥
Most exciting breakthrough: MALDI-FTICR successfully analyzed BURNT BONES! These are typically excluded from ZooMS due to collagen breakdown.
They achieved taxonomic IDs on carbonized specimens where MALDI-TOF completely failed. #archaeology #teammasspec
2️⃣ Key innovation: untargeted approach using ion mobility separation (TIMS) with rapid Evosep LC enables global peptide analysis in minutes rather than conventional 60-min Orbitrap runs. Works for both human and cattle remains. #teammasspec
2️⃣ Team including @fridowelker.bsky.social analyzed predicted enamel proteins from 232 primate species, simulating ancient fragmentation patterns to test phylogenetic signal preservation. Important validation study! #teammasspec
Roman Zubarev and Muriel Priault's 4th Edition of the "Janeiro na Madeira" meeting starts this Sunday
23 talks freely accessible, Zoom link on the website.
jnm-2025.mozellosite.com
Jean-Pierre Changeux (allosteric proteins)
en.wikipedia.org/wiki/Jean-Pi...
kicks off at 17:00 GMT
#teammasspec
Janeiro-na-Madeira #Proteomics Winter Summer School 2025 program is now online
Lectures and actions for EU consortia TopSpec, ARIADNE and ALLODD #teammasspec.
Useful threads like this on #teammasspec is another indication that @bsky.app is coming of age.
Parts came in finally! Very happy with the blue pieces I chose. #teammasspec #chemsky
This manuscript just came out in JBC
I'm glad we got to collaborate with Dr Wysoczynski and Tyler Jobe from his lab. Together with De Silva we provided stable isotope resolved #metabolomics data used in the manuscript.
Link: www.sciencedirect.com/science/arti...
#teammasspec #envirome
Informative and comprehensive review:
Pivotal Role of Mass Spectrometry for the Assessment of Exposure to Reactive Chemical Contaminants: From the Exposome to the Adductome
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
#exposomics #chemicalexposures #teammasspec #exposurescience
Schematic representation of the experimental setup (a) and the peptide identification strategies tested, including the different software, databases, and digestion (b). We systematically investigated the performance of a wide range of sequencing tools and search engines in a controlled system: the experimental degradation of the single purified bovine β-lactoglobulin (BLG), heated at 95°C and pH 7 for 0, 4 and 128 days. We target BLG since it is one of the most robust and ubiquitous proteins in the archaeological record. We tested different reference database choices, a targeted dairy protein one, and the whole bovine proteome and the three digestion options (tryptic-, semi-tryptic- and non-specific searches), in order to evaluate the effects of search space and the identification of peptides. We also explored alternative strategies, including open search that allows for the global identification of PTMs based upon wide precursor mass tolerance and de novo sequencing to boost sequence coverage. We analysed the samples using Mascot, MaxQuant, Metamorpheus, pFind, Fragpipe and DeNovoGUI (pepNovo+, DirecTag, Novor), benchmarked these tools and discuss the optimal strategy for the characterisation of ancient proteins. We also studied physicochemical properties of the BLG that correlate with bias in the identification coverage.
New paper led by Ismael Rodriguez, with @yc386.bsky.social, @rbharathkumar91.bsky.social, @mirandaevans.bsky.social, @jaadekker.bsky.social, @jespervolsen.bsky.social on benchmarking a single degraded protein
out in @PCI Mathematical & Computational Biology
doi.org/10.24072/pcj...
#teammasspec
Found out about this DNA adduct database: sites.google.com/umn.edu/dnaa...
from a manuscript that came out from Turesky, Villalta et al. - Mass Spectral Library for DNA Adductomics pubs.acs.org/doi/10.1021/...
#exposomics #teammasspec #adductomics
A diagram showing different post-translational modifications of the amyloid beta (Aβ) peptide. The top panel displays the Aβ amino acid sequence (1-42) with specific modifications marked in red, including pGlu3, isoAsp7, 3NTyr10, and pSer26. The bottom panel is divided into four quadrants around a central circle labeled "Posttranslational Modifications of Aβ", showing different types of modifications: Pyroglutamylation (top), Truncation (right), Isomerization (bottom right), Phosphorylation (bottom left), and Nitration (left). Each quadrant contains blue helical peptide structures with their specific modifications shown in red, along with arrows indicating the enzymes or processes responsible for these changes (such as NOS2, ADAMTS4, PKA/CK1, and QC/isoQC).
link.springer.com/article/10.1...
🧠 modified amyloid-β variants across different dementias reveals isoAsp7-Aβ as abundant form which correlates with disease severity #neuroscience #alzheimers #proteomics #teammasspec
Love the illustration of the alternative PTMs
Self-promotion for a talk I gave to “Palaeoproteomics and Archaeology, Society for Techniques and Advances” PAASTA
@paastacommunity.bsky.social #teammasspec for #archaeology
Their library of online presentations here
www.youtube.com/@PAASTAcommu...
The community
paasta-community.github.io
