Part 1 was just the beginning 🚀
After the Cryo-EM SPA mini-course kickoff with @royalmicrosoc.bsky.social & ThermoFisher Scientific, don’t miss:
🗓️ Apr 21: Data Collection
🗓️ May 5: Data Processing (with Structura Biotechnology)
Register 👉 bit.ly/4cdEnrr
#cryoEM #CryoSPARC
High-resolution reconstructions don’t come from “more data” – they come from better data.
Join us for Webinar 2 (Data Collection) in our Cryo-EM SPA introductory mini-course, with the Royal Microscopical Society.
🗓️ April 21, 2026 | 6 pm BST
✅ Register once for access to all sessions: ter.li/pksdqb
Close-up of a DNA double helix model with event details for Thermo Fisher Scientific's Cryo-EM SPA mini-course in April and May 2026.
Book now for the Thermo Fisher Scientific Cryo-EM SPA introductory mini-course (14 April, 21 April & 5 May) - FREE online webinar series featuring lectures, Q&A and interactive, virtual break-out rooms 🙂 🔬
Book now (FREE): https://ow.ly/ApaU50YxIf5
The 1st UCSC Cryo-EM Workshop - an intensive, hands-on program dedicated to Single Particle Analysis (SPA) will take place June 23–26 at the UCSC Biomolecular Cryo-EM Facility in Santa Cruz, California - Registration closes April 10th - Learn More -> zurl.co/FE5a4
1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).
SPA success is won (or lost) before you sit down at the microscope.
Join Thermo Fisher Scientific and the Royal Microscopical Society for Webinar 1 of a free, 3-part Cryo-EM SPA introductory mini-course – focused on SPA Sample Preparation.
🗓️ April 14, 2026 | 6 PM BST
Register now: ter.li/pksdqb
At the #cshlcourses cryo-EM course, Rich Waldo from @structurabio.bsky.social gave us a tutorial on cryoSPARC and did a fabulous job! I was honored to be used for an Einstein from noise demo.
We filled day 3 with #cryoEM core knowledge covering foundational math, data analysis workflows and reconstruction practicals.
Thanks to Fred Sigworth, David Jeruzalmi & Collin McManus (#cryoSPARC practical).
Ready for a deeper dive?
cryoemprinciples.yale.edu
@structurabio.bsky.social
Figure 1. Cryo-EM of abundant protein complexes in native membranes.
Figure 2. Cryo-EM of membrane proteins in vesicles.
Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.
Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.
I've written a review on what I think is an extremely exciting direction in cryo-EM:
Cryo-EM of endogenous membrane proteins in their native lipid bilayer
Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...
The University of Waterloo is seeking internationally renowned scholars, especially those working in #CryoEM, for the prestigious Canada Impact+ Research Chairs program- Chairs receive long-term funding & institutional support - Learn More zurl.co/brhwu
Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus!
Deadline fast approaching!
Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!
13/ A huge thanks to all those who helped test and provided feedback during the private beta!
We look forward to your feedback ahead of the general release!
See the full changelog at: cryosparc.com/updates/v5.0.0
Join the conversation at: discuss.cryosparc.com
12/ Updating to v5 includes validation of database contents, taking up to one hour for large instances.
Detailed update instructions, downgrade options and compatibility notes here: guide.cryosparc.com/setup-config...
11/ And finally, v5 connects the #CryoSPARC app with all of the detailed documentation (guide.cryosparc.com) that our team produces, with embedded docs links throughout the interface!
10/ Resource usage (GPU, CPU, and RAM) is now clearly visible across the interface, making it easier to diagnose bottlenecks, optimize scheduling and make better use of shared compute resources.
9/ Additional workflow improvements for #cryo-EM: Tags can now be created inline and applied to multiple jobs at once, parameters can be copied + pasted across jobs, and job info tags (job card stats) can be exported.
8/ Uploading external 3D volumes and masks into CryoSPARC is smoother. When Auto-Import is enabled, CryoSPARC will automatically create and queue an Import 3D Volumes job when .mrc or .map files are uploaded.
7/ In the tree view, upstream jobs are now collapsed into grouped cards, making it easy to stay focused on the jobs relevant to your workspace, and see where jobs from other workspaces are connected.
6/ The Job Dashboard brings all key outputs, plots, and diagnostics into one place. Interpret results at a glance and see changes over iterations. With the multi-job Comparison View, immediately spot differences and make decisions about the effect of a parameter or input change.
5/ v5 updates to CryoSPARC Live in make real-time processing easier under real-world data collection and hardware constraints. Delay the start of a Live session until new data arrives, auto pause when no new data has been found, and run multiple preprocessing workers per GPU.
4/ Homogeneous Ab-Initio Refinement enables refinement to high resolution from scratch, while preserving gold standard independence. Inspired by @olibclarke.bsky.social group’s results showing that Ab-Initio Reconstruction can improve map quality for small challenging targets.
3/ 3D refinements now perform per-particle scale optimization by default. The optimized values measure particle quality, used during reconstruction to down-weight low quality particles. The values can also be useful for separating low-quality particles from a dataset.
2/ Masking during 3D refinement has been significantly reworked. Dynamic mask generation now adapts automatically as resolution changes, reducing the likelihood of overfitting or overestimated resolution, without users needing to modify parameters.
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
Interested in learning or strengthening skills in #CryoEM single particle analysis? Apply for the CSHL cryo-EM course happening in March! This is a super intense 2 week course covering theory up through data deposition. Apps due Jan 9! Info here: meetings.cshl.edu/courses.aspx...
Exciting collab with Paul Palme, Peter Imming, & Adrian Richter!
Guided by @courbongautier.bsky.social's structure of myco ATP synthase bound to a squaramide, they have made SQAs that are less toxic, more potent, more stable than AstraZeneca's SQ31f
#MedChem #TB #NTM
pubs.acs.org/doi/full/10....
Featured #paper shows mechanism of #malaria infection blocking. Read "Cryo-EM structure of endogenous Pfs230:Pfs48/45 complex with 6 antibodies reveals mechanisms of malaria transmission-blocking" by @utoronto.ca using our CMCF beamline. https://bit.ly/47TWrVv 👈
🚀 Hiring: Cryo-EM Application Scientist, Marketing!
Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems.
structura.bio/careers/appl...
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
