(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen!
Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org!
doi.org/10.1073/pnas...
👇🧵
An electron micrograph reveals numerous bacteriophages attached to a single bacterium. These viruses, far outnumbering any other life form on Earth, are potential allies in fighting bacterial diseases. The image showcases their minuscule size and unique structure, highlighting the phage's protein coat and genome (DNA or RNA).
Astronomy Picture from 21/04/2008
Bacteriophages: The Most Common Life-Like Form on Earth
Source: https://apod.nasa.gov/apod/ap080421.html
#Bacteriophages #Viruses #ElectronMicroscopy #Microbiology #Astronomy #Space #Science #Bacteria #Phages #TinyWorlds
Apply for a position as Postdoctoral Research Fellow in Fish Immunology/Microscopy @biovitenskap.bsky.social
This role contributes to the ERC-funded project “Fish-S.H.I.E.L.D.”, which investigate the fish immune surveillance system and its importance for the fish defense against infectious diseases
We are proud to announce the launch of FAIR Image Analysis Across Sciences, supported by the OSCARS project, in collaboration with Simula Research Laboratory, #EPFL & University of Bergen (UiB)! 🎉
Vi har mange ledige #PhD-stillinger ledige ved @biovitenskap.bsky.social med søknadsfrist 28. februar 2025 Kanskje noen av stillingene er aktuelle for deg eller noen du kjenner? Ta en titt og del gjerne!
www.mn.uio.no/ibv/om/ledig...
We have a PhD position available at the Department of Biosciences, University of Oslo to work on peptide signaling in plants and insects. Please share.
www.jobbnorge.no/en/available...
For the first time in plants, we used APEX-based electron microscopy to map the precise localization of ATG8 at the vacuolar membrane after stress! 🚀
Pushing the boundaries of plant cell biology—one EM image at a time. ⚡👀
#PlantScience #ElectronMicroscopy #Autophagy #Vacuole #NaturePlants
#Correlative Light and Electron Microscopy, #CLEM, is a modern #method combining light and #electronmicroscopy data. Although it is considered “new”, it was already used in the early 1960s, for instance to study the #ultrastructure of mitotic cells in this beautiful example: doi.org/10.1083/jcb....
sauerkraut with a variety of bacteria magnified 8,000x
I fear there is deep truth here.
“One of the challenges in science journalism is the oversimplification of research findings to attract attention. Headlines such as “scientists find cure for cancer” or “ozone layer is healing” are designed to attract readers’ attention but often misrepresent the complexity of scientific research.”
This is probably what a #rotifer gets to see when it encounters a (seemingly bad-tempered) 6dpf #zebrafish larva. #Creepy! But maybe the fluffy #nostrils or the funny #neuromast beard make the situation a little better...
Scanning #electronmicroscopy / #SEM
Still our favorite #review! Unfortunately, as relevant today as when it was published, it is an excellent illustration of the #problems arising from the neglect of #ultrastructure and #electronmicroscopy in #cell-biology and the over-reliance on #fluorescence #microscopy.
doi.org/10.1016/0962...
electron microscopy picture of a microbial cell
New #ISEPpapers! The nature of ‘jaws’: a new predatory representative of #Provora and the ultrastructure of nibbling protists royalsocietypublishing.org/doi/10.1098/...
#Protists #Microbes #Biology #TreeOfLife #Microscopy
#Science in the age of #selfies
A short #opinion article that we think hits a very important point!
Albert #Einstein: “an academic career, in which a person is forced to produce #scientific writings in great amounts, creates a #danger of intellectual superficiality”
www.pnas.org/doi/10.1073/...
Dynamic duo at the cell's edge: Caveolae and the actin cytoskeleton work together to sense and respond to mechanical stress. Caveolae buffer tension, while actin provides structure and force. Ultimate biomechanics team. #CellBiology #Mechanotransduction
Instead of listing my publications, as the year draws to an end, I want to shine the spotlight on the commonplace assumption that productivity must always increase. Good research is disruptive and thinking time is central to high quality scholarship and necessary for disruptive research.
At my institution, they want to install profiles on our personal phones that will give IT root level access to our personal phones.
And allow them to remotely wipe our phones. Our personal phones.
Damn straight, take your work email off your phone. And remove your work profiles from your phones.
Safer, nonradioactive staining alternatives for electron microscopy are introduced, simplifying sample preparation, reducing costs and making structural biology more accessible to researchers globally #NegativeStaining #ElectronMicroscopy doi.org/10.1107/S205...
A warning textbox that says: "Warning These files have been contained because you tried to copy sensitive data to removable or network media. This is a violation of your organization's policy. You can save the file to an authorized location or permanently delete it. Click here to view the file and save it to a new location."
This is our IT department telling us that we cannot copy confocal microscopy data to our Imaris workstation so that we can... analyze the confocal data.
Did you know that just 100-200 gold particles in your #immuno-EM data can give you an incredibly good idea of the #labelling distribution and reveal positive signals that you may not immediately recognise? It's amazing how reproducible this " #100-gold-method" is. #stereology
doi.org/10.1369/jhc....
Join us for the last Pub before the Winter Break! ☃️
We'll learn about #VolumeEM approaches from the experts at #EuroBioImaging’s Advanced Light & Electron Prague Node, part of @volumeem1.bsky.social community.
🗓️Fri, Dec 20 @ 13:00 CET
All are welcome 🔽
www.eurobioimaging.eu/events/diver...
I wrote some thoughts about why peer review matters
It shapes scientific standards, maintains field coherence & trains new researchers
Yes, it needs improvement—but it's the glue that holds scientific progress together
briscoelab.org/2024/12/11/i...
A screenshot of the Pubcrawler logo and motto. The motto - "It goes to the library - you go to the pub" - must be the best in the business.
🧪🎅 12 Days of Tools for Science Management 🦠🎄
Day two: pubcrawler.gen.tcd.ie
Written by the amazing Karsten Hokamp, Pubcrawler is a customizable alert system for newly-published manuscripts that gives you power over type and frequency of alert. Many other tools exist, but none with as much neon!
The toxic, #arsenic -containing #Cacodylate buffer is still used in many #electronmicroscopy labs today. Although it is a good buffer, it is no longer needed today as there are better and safer alternatives that perform just as well - #PHEM buffer (Schliwa, 1981) for example.
doi.org/10.1111/j.13...
SEM image of a rhizarian testate amoeba (euglyphid) showing its glass scales.
Not a fish or a dragon scale, but a single cell of a #protistsonsky. This rhizarian testate amoeba creates perfect tiny glass scales to build it's shell. From a peat bog. #protistaday.
I'm gonna piss a few nerds off but this is an example of why a lightsheet microscope should be used to study organoids. The detection optics are great for imaging in XY but they suck axially. Sure, yeah, hocus pocus, RI matching, correction collar, blah blah, but it's physics, baby.
Last week I showed to MSc students this iconic movie of a #leukocyte chasing a #bacteria, just wait...
Version taken from a 16 mm video made by David Elliot Rogers @VanderbiltU in the 1950s !
(as @mag2art.bsky.social says "the Citizen Kane of #Microscopy)
#CellMigration @focalplane.bsky.social
