Some of my PhD work is now out in Nature!
www.nature.com/articles/s41...
Investigating the structural basis of off‑target Cas9 binding to supercoiled DNA with #AFM & cryo-EM. From my time in the lab of @alicepyne.bsky.social, in collaboration with @qmsmith.bsky.social and the team at the MRC LMS.
Supercoiling not only brings DNA to life (and makes it dance) but also changes how key proteins such as #CRISPR interact with it. Thanks @qmsmith.bsky.social for the brilliant collaboration, Sylvia for developing an amazing new #imageanalysis pipeline & @eddierollins.bsky.social for beautiful #AFM.
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵
🔗 www.nature.com/articles/s41...
#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
New research in Nature shows that the physical twisting of DNA plays an important role in DNA editing mistakes made by CRISPR.
Using nanometer-sized circles of DNA, scientists captured never-before-seen interactions between CRISPR & DNA, providing insights that could eradicate errors altogether.
2nd Flyer includes names & titles of the SMBio26 Sheffield selected speakers
Organized with the @sheffielduni.bsky.social team: Matt Newton, @alicepyne.bsky.social & Ruby Peters.
👏 Speakers include Gijs Wuite, @jwelburn.bsky.social, @slfort.bsky.social, @akellett.bsky.social, @michellepeckham.bsky.social & Aakash Basu.
Program & registration --> bit.ly/3NtxZlM
📐On #WorldMetrologyDay, we celebrate 150 years of measurement science! At #SPM40, precision at the nanoscale is our mission, advancing biomedical research through AI-powered microscopy. #MetrologyMatters #MSCA #ResearchEU
Abstract deadlines coming up soon!
Talk - 14th March
Poster - 28th March
Sign up on our Eventbrite: www.eventbrite.co.uk/e/glasgow-im... and come along and see our wonderful keynote speaker @alicepyne.bsky.social !
For more info, head to the GIN website : glasgowimagingnetwork.wordpress.com
Micrograph of keratin networks (green) and DNA (blue) within cells Image by Rachel Lawson
🚨 PhD position available for Oct 25
🔬Using advanced microscopy to understand how keratin networks control tissue mechanics
An interdisciplinary #cellbiology #biophysics collaboration with Ruby Peters & @alicepyne.bsky.social
👇 Apply by March 14
www.findaphd.com/phds/project...
The Peak District from Stansted edge at sunset with a whippet looking out
It’s been a busy start to the year with marking, paper and grant reviews and travel to SMB2025 in the US, I think we were delayed for as long as we were actually at the conference…
Its good to be home in the Peak District with my best girl Tasso 🌅 🐕 ❤️
After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service.
@jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases
Details: cdn.rcsb.org/rcsb-pd...
Delighted to share our @NAR_Open paper on a click chemistry-derived DNA damaging agent 🧬✂️👏 Congrats to @westerlundlab.bsky.social @alicepyne.bsky.social @researchireland.bsky.social @dcuresearch.bsky.social @sspc.bsky.social @dublincityuni.bsky.social doi.org/10.1093/nar/...
Two more weeks to apply to our opening for a senior postdoc in biophysics; work on exciting projects in DNA replication and/or chromatin organization from an interest in instrumentation! Deadline Jan 15 and application link: my.corehr.com/pls/uoxrecru...
I know - there’s a huge tree uprooted just after the stepping stones too - hope you’re having a lovely winter in the Southern Hemisphere!
A path with failed broken trees by the side
Today a Tasso jog through Endcliffe park - lots of storm damage with trees uprooted 🐕
A man and a dog in a frost field with the sun just above a hill
Frosty twixmas adventures with Tasso 🤍💚
A cloud inversion in the valley of the Peak District
A cloud inversion behind some fields in the Peak District
A few days of beautiful cloud inversions in the peak. Today a little exploration by bike 🚲- trying to stay above the cloud
Whippet Tasso in a green field under a bright blue sky
Boxing Day walks with Tasso, enjoying the #PeakDistrict and thinking how much my mum would have loved to have been with us 💚
View of the Peak District with the sun breaking through the clouds
First Christmas without maman, thinking of her lots and spending time enjoying the Peak District. She loved it up here 💚
Sunrise in the woods through the trees
Celebrating the start of longer days with a beautiful sunrise on a morning trot with Tasso 🐕
Only two days left to apply for this amazing opportunity to join Siân’s lab at Kings. Interested in data analysis, microscopy and working with an awesome PI? One not to miss 🔬 💻
We have a #PhD available! I'm really excited about this project, looking at whether and how differences in the #IDRs of paralogous epigenetic enzymes CBP and p300 cause them to behave differently. www.findaphd.com/phds/project...
📣 Please share: We’re hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...
Looking for a #biophysics PhD? There's still time to apply for our Yorkshire Biosciences project on how keratin networks affect the mechanical properties of cells & tissues. Interdisciplinary collab with Ruby Peters & @alicepyne.bsky.social.
Deadline 6th Jan
www.findaphd.com/phds/project...
On my way to London for the @royalmicrosoc.bsky.social Data Analysis in Microscopy section meeting. Excited to catch up in person with everyone and chat all things 🔬 💻
Gosh it’s beautiful isn’t it! See you there!
A butterfly shaped croissant
A weekend of catching up with old pals from UCL in Hamburg. Tackling the grey drizzle with epic pastries 🥐
We were so happy to have @raveentank.bsky.social join us for our first Insigneo Imaging Life workshop in Sheffield this week. It was an amazing day of microscopy🔬 and science 🧪 - thank you Raveen for braving the Manchester-Sheffield train line!
1/ Cancer cell biology MRC DiMeN project in collaboration with @maiblab.bsky.social & Liz Smythe to use recombinant biosensors to image active KRAS in #pancreaticcancer
Apply by 13th Dec
www.findaphd.com/phds/project...
In this paper #AFM is used to image the chain-mail structure of kinetoplast #DNA from parasites and automated analysis unravels the complex molecular topology. 🧪 🔬 🧬
@alicepyne.bsky.social doi.org/10.1039/D4CP...
The @royalmicrosoc.bsky.social #DataAnalysisInImaging special issue of Journal of Microscopy is out now! onlinelibrary.wiley.com/toc/13652818...
#ImageAnalysis #BioImageAnalysis #RMSDAIM