New #preprint 😍‼️ led by 2 incredible postdocs @ninizhani.bsky.social & Ranj Papareddy: transforming #UFMylation from a local ribosome rescue pathway to systems level regulator of mRNA splicing www.biorxiv.org/content/10.6... A short 🧵

Congratulations! Great work 👏

Excited to share my first PhD student’s @biorxivpreprint.bsky.social!

Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!

Check it out!

www.biorxiv.org/content/10.1...

Congratulations again, Xiao-Xiao! It is a pleasure to host you as a Humboldt Research Fellow @humboldt-foundation.de. Looking forward to the exciting science ahead!

Synthetic fluorophores for live-cell fluorescence microscopy and biosensing - Nature Chemical Biology Performing live-cell microscopy experiments with high spatial and temporal resolution requires fluorophores with highly optimized properties. This Review examines the progress in developing synthetic ...

A new Review by Agnese Nicoli and Michelle Frei examines the progress in developing synthetic small-molecule fluorophores and how these in combination with innovative labeling strategies can advance chemical biology

www.nature.com/articles/s41...

Directed evolution of LaccID for cell surface proximity labeling and electron microscopy - Nature Chemical Biology LaccID, an engineered laccase, enables hydrogen-peroxide-free proximity labeling and electron microscopy (EM) in mammalian cells. Notably, LaccID is selectively active at the cell surface, enabling th...

A new paper from @aliceyting.bsky.social reports on LaccID, an engineered laccase, that enables hydrogen peroxide-free proximity labeling and electron microscopy of cell surfaces

www.nature.com/articles/s41...

Finally out!
I’m thrilled to share our new paper (Wolin et al., Cell 2025).

This paper describes SPIDR, a high-throughput method for mapping RBP binding sites.

By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit!

tinyurl.com/ydn6e3ac

Read the News & Views highlighting this work, here!

www.nature.com/articles/s41...

Developments of the mDZ-RhoBAST Probe for Super-Resolution Imaging of FTO Demethylation in Live Cells Single-molecule localization microscopy (SMLM) provides precise, high-resolution visualization of target biomolecules in cellular imaging. Fluorescent light-up aptamers (FLAPs) offer a versatile appro...

Neat paper in JACS. They use a modified version of RhoBAST to do super-res imaging of demethylation of m6A by FTO pubs.acs.org/doi/10.1021/...

Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - Nature Biotechnology Far-red kinase biosensors are applied to image kinase signaling networks at super resolution.

Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social!
www.nature.com/articles/s41...

A highly stable monomeric red fluorescent protein for advanced microscopy - Nature Methods The red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el...

www.nature.com/articles/s41...

Single-molecule live-cell RNA imaging with CRISPR–Csm - Nature Biotechnology Single-molecule live-cell fluorescence in situ hybridization uses an RNA-targeting CRISPR–Csm complex to image and track endogenous RNAs.

Single-molecule live-cell RNA imaging with CRISPR–Csm - @doudna-lab.bsky.social @qb3-berkeley.bsky.social @innovativegenomics.bsky.social go.nature.com/3EJPKsM

De novo design of transmembrane fluorescence-activating proteins - Nature A study describes the design of de novo ligand-binding transmembrane proteins, demonstrating their specific binding and activation of fluorogenic ligands.

Nature research paper: De novo design of transmembrane fluorescence-activating proteins

https://go.nature.com/4k2OAZA