I’m super excited to share that my PhD work is now out in npj Imaging (@natureportfolio.nature.com)! 🔬

You can read the paper here: rdcu.be/eOKdU

The culmination of years of hard work by Anna Maria Reuss & Co: aDISCO - a protocol for clearing & antibody-staining human formalin-fixed paraffin-embedded (FFPE) tissue! With lots of @mesospim.bsky.social #lightsheet #Microscopy @ptrrupprecht.bsky.social & many more!
www.biorxiv.org/content/10.1...

Lymphatic vessels traced to their origins from a specialised pool of cells called angioblasts

📷: Irina Lupu @degrainger.bsky.social Nils Kirschnick et al @oliverastone.bsky.social Friedemann Kiefer
@ox.ac.uk @uni-muenster.de @naturecvr.bsky.social

➡️: bpod.org.uk/archive/2025...

Reducing Neutrophil Extracellular Traps cuts risks (stroke, bleeds & neurological problems) in tumour-like brain vascular lesion cerebral cavernous malformation

Image with @leicamicrosystems.bsky.social

📷: Favour Onyeogaziri et al @uu.se
@naturecvr.bsky.social

➡️: bpod.org.uk/archive/2025...

3D reconstruction of a 3-day-old #zebrafish heart beating. Credit to Michael Weber (prev. @mpi-cbg.de). #ZebrafishZunday 🧪

A Groundbreaking Way to Break Ground An earthworm-inspired robot could make undergrounding of power lines cheaper and safer, reducing the risk of future wildfire outbreaks.

An earthworm-inspired robot could make undergrounding of power lines cheaper and safer, reducing the risk of future wildfire outbreaks.

3D ultrastructure of blood stages of the #malaria parasite reveals organelle details as potential treatment targets

📹: Felix Evers et al Taco Kooij lab Radboud University Medical Center, Nijmegen in @naturecomms.bsky.social

➡️: bpod.org.uk/archive/2025... with
John Ankers

This is our best in vivo AP propagation movie so far. Recorded at 1k Hz from the axonal branches of a fly CNS neuron, using voltage indicator pAce.

Scientists have designed an “ingenious” side-view device that lets them observe the structural changes of 3D tumour spheroids more comprehensively.
elifesciences.org/articles/101886

I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope:
-> Mechanically and thermally stable.
-> Relatively straightforward to align.
-> 10 popular configurations.

Make your own:

github.com/amsikking/SO...

Or buy it from @jsdaniel02.bsky.social at ASI!

Previously undescribed role found in zebrafish for transcription factor Sox10 in initiating skeletal mineralization

📷: Stefani Gjorcheska et al Barske lab
@cincyresearch.bsky.social in @dev-journal.bsky.social
➡️: bpod.org.uk/archive/2025...

#Pericyte - #EndothelialCell interplay in 3D Microvessel (160-μmφ hBMMSC HUVEC) in tunable Collagen Hydrogel

Low-temp 4°C polymerized gel➡️stiff-bundled collagen fibers➡️
⏬Pericytic differentiation (⏫NOTCH3 TGFβ1)
⏫Sprouting
⏫Permeability
⏫#Pericrine IL-8

#SciAdv 2025
www.science.org/doi/full/10....

A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo. It works well with wide-field imaging, two-photon microscopy, and fiber photometry biorxiv.org/content/10.1...

This video shows a subset of ribosomes inside a cancer cell actively translating mRNA molecules.

Each point of light is one ribosome. An image was taken every 3 minutes over a 4-hour period.

Details protocols for
1) Micro-ultrasound imaging of 🐭 #SuperiorMesentericArtery Vevo 2100 @VisualSonics
2) Isolation of 🐭Abdominal aorta with ALL major branches😍

#STARProtocols 2025
www.sciencedirect.com/science/arti...

Identifying Higd1b as unique specific marker of pericytes enables subtypes & their roles to be distinguished in detail

📷: Timothy Klouda, Yunhye Kim & Seung-Han Baek et al
@bostonchildrens.bsky.social in
@embojournal.org

➡️: bpod.org.uk/archive/2025...

We just released and uploaded a new version of *Mastodon*, the large-scale tracking and track-editing framework for large, multi-view images.

Mastodon is available via Fiji, via a dedicated update site.
mastodon.readthedocs.io/en/latest/do...

Below is a thread that survey some of the novelties ⏬️

Researchers have broadened the spectrum of available bioluminescence colors.

The new method allowed the team to captured a single image of 20 different bioluminescence color samples with an ordinary smartphone camera.

Read the OPN story: bit.ly/3WFO5KR

#color #light #bioluminescence #imaging

LIFE FINDS A WAY!!!

www.sciencedirect.com/science/arti...

Organ-on-a-chip model of changes in collagen stiffness around capillaries reveals role of perivascular cells in vascular response in health & disease

📷: Cristiane M. Franca et al Luiz E. Bertassoni lab
@ohsunews.bsky.social in Science Advances @science.org

➡️: bpod.org.uk/archive/2025...

CellAge
Database of Cell #Senescence Genes
genomics.senescence.info/cells/

279 Senescence-associated genes
869 Longevity-associated genes

A multidimensional systems biology analysis of cellular senescence in aging & disease

#GenomeBiology 2020
genomebiology.biomedcentral.com/articles/10....

The flyer for F-BIAS open-desks.

France-BioImaging open-desks in #bioimage-analysis!

Are you an imaging scientist working with a core-facility of @france-bioimaging.bsky.social ?
We organize bi-monthly consultation sessions for researchers working in a lab in France, supporting their project with bioimage analysis

World’s first fully 3D-printed microscope made in under 3 hours The microscope cost less than £50 to build using an open-source design and a common 3D printer

Our fully 3D-printed microscope is featured in @newscientist.bsky.social www.newscientist.com/article/2462...

We built on the @openflexure.bsky.social platform to combine a 3D printed microscope chassis with both a 3D printed condenser and objective lens.

Now we have the entire microscope, including the optics, printed for less than £7.00… and it works!

(2/N)

1/6 🧵 I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$16). My goal? A complete spectrometer that is barely larger than a sample cuvette itself.

A composite image of an OpenFlexure Microscope from 3 angles

We have just released a new beta of our long awaited Version 7 hardware release! 🔬

Assembly instructions and all STL files available here:
build.openflexure.org/openflexure-...

#OpenSource #OpenHardware #OpenScience #Microscopy

Intravital two-photon microscopy of the native mouse thymus The thymus, a key organ in the adaptive immune system, is sensitive to a variety of insults including cytotoxic preconditioning, which leads to atrophy, compression of the blood vascular system, and a...

Our paper "Intravital Two-Photon Microscopy of the Native Thymus" demonstrating in vivo imaging of the native thymus for the first time is finally out.
You can read it here: journals.plos.org/plosone/arti...

#openµView: A portable #RaspberryPi-based #OpenSource desktop #microscope:

- brightfield/oblique/dark-field & #Rheinberg illumination
- touchscreen display
- #LED ring
- #PiCam

doi.org/10.1016/j.oh...
#DIYbio #lab #instruments #imaging #optics #microscopy #MINT #STEM #education