What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~
www.biorxiv.org/content/10.6...
An ovarian follicle poised for ovulation, with the oocyte at its centre. Cell membranes are shown in orange and DNA in blue.
How does an ovarian follicle remodel in space and time to release an egg? 💥🥚
We have an exciting PhD project combining follicle culture, advanced imaging and quantitative analysis to understand the cellular dynamics of ovulation. 🔬
Co-supervised by me and @guignardlab.bsky.social. Please share! 🌱
1/ 🔬🧑💻 Tired of going back and forth between GUIs and code for your 3D image analysis needs? Would you like to keep your focus in one place (notebook), without sacrificing interactivity? Here’s a tool that can help!
Our latest work on shape-programmable tissues is out in @science.org. By positioning topological defects in cellular nematics, we encode frustrated 2D force fields that relax into predictable 3D shapes. Collaboration with Marino Arroyo’s lab, led by @pauguillamat.bsky.social at @ibecbarcelona.eu.
Mapping nerves in a whole embryos.
We find that across species and development stages, embryonic nerves display (beautiful) fractal geometry.
More here: www.biorxiv.org/content/10.6...
It's funky axons time 🔬 🧪
Can you guess why the microtubules are organized so differently at the bottom and top in this gigantic COS cell? (a game for you @manuelthery.bsky.social)
A sneak peak of a complex and technically challenging experiment that my lab developed: doi.org/10.1096/fj.2.... Super proud of PhD candidate Hannah Johnson for showcasing our Whole-gut spatial genomic analysis in #zebrafish. This video illustrates one landmark in the protocol after multiple 1/3
This developing quail embryo looks like it's thinking fiery thoughts for #FluorescenceFriday 🐣🔥🧪. Imaged by the fantastic @vanderspuy.bsky.social
Microscopy image of a kidney only showing the highly branched arterial tree in magenta against a black background.
The beauty of the kidney arterial tree for this #FluorescenceFriday. Evans Blue dye labels the vasculature. Image courtesy of postdoc Sarah McLarnon.
1/2
Happy #FluorescenceFriday 🔬
Another cryosection of a dissected zebrafish gill:
⚪Phalloidin
🔵DAPI
The first image I took of 2026 #FluorescentFriday
Latest work from the lab by Aude Nommick et al., in which we propose a "size-scaling" model for microtubule force exertion that regulates centrosome centration vs decentration during embryo development!
@ijmonod.bsky.social
www.biorxiv.org/content/10.6...
So many tuft cells (yellow/orange) for #TuftTuesday. Immune cells are in green.
Beautiful images from @christlet.bsky.social labelling tubulin in neurons #journalclub
www.biorxiv.org/content/10.6...
A protein called Uninflatable acts as a switch between glial cells growing and glial cells wrapping themselves around axons.
🔗 buff.ly/WRdybO3
Excited to share Alison's @alisonkickuth.bsky.social paper from the lab out in @nature.com this week! We uncovered how a mechanical ratchet mechanism drives cytokinesis in early #zebrafish embryos. Read more in this thread 🧵 and at www.nature.com/articles/s41... 🤩
@poldresden.bsky.social @mpi-cbg.de
Diffusion first, Binding second
Organ-scale uniform immunostaining in 1 DAY🤠, via temporally controlling antibody reaction strength
CuRVE + eFLASH
🐭👤Brain
62 Ab tested
👉deoxycholic acid + pH
👉compatible with transgenic fluorescent proteins
#NatBiotech 2025
www.nature.com/articles/s41...
New paper out!
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology
Hoping everyone's new year fluorescence is clear and bright. Happy #FluorescenceFriday.
New Perspective from myself, Sarah Heilbronner and @myoo.bsky.social . “Rethinking the centrality of brain areas in understanding functional organization” in Nature Neuroscience. 🧵
rdcu.be/eVZ1A
I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy
Confocal microscopy image of a juvenile sea star (Patiria miniata) viewed from the oral side. The animal has a five-armed, star-shaped body with a central nerve ring. The nervous system is labeled in green, forming radial nerve cords extending into each arm, and cell nuclei are labeled in red throughout the animal. The image appears against a black background and has a holiday-ornament-like appearance.
Felt a little festive at the microscope this morning for #FluorescenceFriday 🎄
Here’s the nervous system of a juvenile sea star ⭐️
Green = acetylated tubulin, red = nuclei
Happy holidays!
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Depth-coded somatosensory axons and a plethora of mpeg1+ immune cells (cyan) in the adult zebrafish skin.
Network of somatosensory axons and immune cells in the adult zebrafish skin. Credit to @errricpeterman.bsky.social. #ZebrafishZunday 🧪
I am presenting at the Science and Art Minisymposium at #CellBio2025 today! The line up is great this year. I am hands down the least qualified person presenting. It is a weird feeling but I am excited! Here is my title slide.
Room 122 4pm
since you made it all the way to then end - we've already made transgenic lines with the mSG from Ando et al - it's amazing! here's a movie from our Cell Observatory project at the Advanced BioImaging Center 7/7
Our Science paper is out!
Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group
News: bit.ly/4avnkAr and bit.ly/3XBGVHS
Great perspective by @vram142.bsky.social +K Zhang
For #FluorescenceFriday - RNAscope marking subfields of the hippocampus in a section of postmortem human 🧠 tissue. Image credit to the Imaging Development team @lieberinstitute.bsky.social 🔬🧪👩🔬
