#UNIPV #ArmeniseFoundation @veluxstiftung.bsky.social #AIRC

Molecular structure of the third immunoglobulin domain (Ig3) of human Muscle-Specific kinase (MuSK) The Muscle-Specific Kinase (MuSK) is a monotopic transmembrane receptor responsible for key signaling events during development and maintenance of neu…

Enjoy the paper! - Kudos to Anselmo Canciani for the beautiful accompanying graphics. PDB structures will be released soon.
www.sciencedirect.com/science/arti...

And so many metal ions! - some of which matching sites recently visible in another paper describing a different structure containing the same donation, but not discussed there. a hint for #physiological relevance?

Together with Anselmo Canciani we refined the model and completed the structural analysis. Beautiful crystal contacts, likely suggesting hotspots for #molecularinteractions.

Structure determination was straightforward using #experimentalphasing, as well as modeling of the 10 copies of the domain in the ASU, arranged antiparallel as "Ig domains on a skewer". What a beautiful crystal lattice!

Anomalous signal was strong, and the #XRF spectrum revealed #potassium tightly bound to the protein.

So we tried long wavelength #SAD at #SLS @psi.ch #X06DA, and collected a massive dataset by tilting a crystal multiple times, reaching redundancy >100! many thanks to Vincent Olieric for teaching us how to properly handle the #prigo goniometer and the go scripts for data.

Martina Palamini produced the protein during her #PhD, and got the beautiful crystals, that diffracted nicely at @esrf.fr. The unit cell was huge, and MR was painful.

In this work, we characterized the third Ig domain of human MuSK, a #cellsurface #receptor #protein #kinase critical for #neuromuscularjunction formation and stabilization.

Can the #Xray #crystalstructure of a single immunoglobulin domain still hold interesting scientific surprises in the Alphafold era?

Molecular structure of the third immunoglobulin domain (Ig3) of human Muscle-Specific kinase (MuSK) The Muscle-Specific Kinase (MuSK) is a monotopic transmembrane receptor responsible for key signaling events during development and maintenance of neu…

Excited to share our latest work, published today in #JSB!
www.sciencedirect.com/science/arti...

Big day today with our PhD student Matteo De Marco defending his thesis about #collagen #glycotime #enzymes within the PhD program of Genetics, Molecular and Cellular Biology at the University of Pavia #proudPI

Summer holidays mood: on! - and a Great Birthday celebration: sushi lunch with all fellows and colleagues still active in the lab on August 7...

Enzymatic craftsmanship in collagen glycosylation - Nature Communications Researchers comment on two recent Nature Communications publications on enzyme complexes involved in collagen glycosylation, discussing how these findings lay a foundation for understanding collagen I...

Check the great commentary on @natcomms.nature.com from Trayambak Basak and colleagues about our work on #collagen GLT25D1 and the paper from Cao et al. about the LH3/GLT21D1 complex! #glycotime

www.nature.com/articles/s41...

Collagen IV Biosynthesis: Intracellular Choreography of Post-Translational Modifications Collagen IV, an essential and evolutionarily conserved component of basement membranes, is one of the most extensively post-translationally modified p…

Nothing better than seeing our latest review about #collageniv biosynthesis appearing online during the #collagen #GRC!

Super nice #dreamteamwork with @collagenyoshi.bsky.social, Rachel Lennon, Johanna Myllyharju, Antti Salo

www.sciencedirect.com/science/arti...

I also had fun in making gummy GLT25D1 making #notthecover using #blender

Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 - Nature Communications Hydroxylysine galactosylation is essential for collagen maturation. This work reveals the molecular structure of human GLT25D1, its catalytic residues, and a noncatalytic structural domain stabilized ...

Excited to share our new Nature Communications paper describing the molecular structure and the biochemical characterization of human #collagen #galactosyltransferase GLT25D1/COLGALT1 #glycotime www.nature.com/articles/s41...

Carne coltivata: 16 scienziati rispondono alla manifestazione di Coldiretti In una manifestazione promossa da Coldiretti, principale organizzazione degli imprenditori agricoli italiani, che si è svolta mercoledì 19 marzo a Parma, si sono radunate, secondo gli organizzatori, 2...

Coldiretti chiede per la carne coltivata procedure di autorizzazione equiparabili a quelle previste per i farmaci: 16 scienziati ed esperti che affermano che la richiesta non è basata su dati scientifici

www.scienzainrete.it/articolo/car...

Flyer for the 2025 Collagen Gordon Research Conference

Registration for the 2025 Collagen Gordon Research Conference is now open: www.grc.org/collagen-con...
Looking forward to seeing many of you there this summer! #CollagenGRC #TheMatrixIsEverywhere

The lecture room is really packed for this @thermofishersci.bsky.social #cryoem roadshow in Pavia today!

Extremely proud of our #postdoc Riccardo Piccinno, who got a young researchers' #grant from #FondazioneCariplo to develop a challenging #multidisciplinary project at #UniPV #DBB_unipv #insectbiology www.unipv.news/notizie/gran...

A packed lecture room full of #molecularbiology students following our friend and today's guest @iosonoirene #IITGenova lecture about Diving into the #3DGenome architecture @DBB_UniPV @unipv @ArmeniseHarvard

This year we joined @fondaz_inf_act to play games and discuss the science of #infectiousdiseases at @sharpernight @unipv @DBB_UniPV #PNRR @mur_gov_

Tomorrow... #ECM34 @fondaz_inf_act https://x.com/MFPronker/status/1827956491581567142

Amazing visit to the @harvardmed #CryoEM facility. @ArmeniseHarvard

A bit windy here in #Boston, ready to start with the #science program of @ArmeniseHarvard symposium about visualizing #biology at #nano and #macro scales!

Our graduate student @marcoberlinguer proudly showing his #CryoEM approaches to study #mosquito #biology at the dissemination event preceding #febs2024 @DBB_UniPV @unipv @mur_gov_ #PNRR @fondaz_inf_act

Molecular Structure and Enzymatic Mechanism of the Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 During biosynthesis, collagen lysine residues undergo extensive post-translational modifications essential for the stability and functions of collagen supramolecular assemblies. In the endoplasmic reticulum, two distinct metal ion dependent enzyme families (i.e., multifunctional lysyl hydroxylases-glucosyltransferases LH/PLODs and galactosyltransferases GLT25D/COLGALT) alternatively operate on collagen lysine side chains ultimately generating the highly conserved α-(1,2)-glucosyl-β-(1,O)-galactosyl-5-hydroxylysine pattern. Malfunctions in the collagen lysine post-translational modification machinery is linked to multiple developmental pathologies as well as extracellular matrix alterations causing enhanced cellular proliferation and invasiveness of several solid tumors, prompting for an in-depth characterization of LH/PLOD and GLT25D/COLGALT enzyme families. Here, we present an integrative molecular study of GLT25D1/COLGALT, highlighting an elongated head-to-head homodimeric assembly characterized by an N-terminal segment of each monomer wrapping around its dimerization partner. Each monomer encompasses two Rossman fold-type domains (GT1 and GT2) separated by an extended linker. Both domains were found capable of binding Mn2+ cofactors and UDP-α-galactose donor substrates, resulting in four candidate catalytic sites per dimer. Site-directed mutagenesis and biochemical studies identify the C-terminal GT2 domain as the functional GLT25D1/COLGALT1 catalytic site, highlighting an unprecedented Glu-Asp-Asp motif critical for metal ion binding, and suggesting structural roles for the N-terminal GT1 essential for correct quaternary structure assembly. Conversely, dimerization was not a requirement for GLT25D1/COLGALT1 enzymatic activity in vitro , suggesting that the elongated enzyme homodimer assembly, resembling that of LH/PLOD binding partners, could represent a functional hallmark for correct recognition and successful processing of collagen lysine residues. ### Competing Interest Statement The authors have declared no competing interest.

Excited to share our new @biorxivpreprint describing the integrative molecular characterization of GLT25D1/COLGALT1, a #collagen #glycosyltransferase with many unusual features: www.biorxiv.org/content/10.1101/2024.06....

Registration form for event 20 June 2024- 2pm BST (9am NY / 10am Canada / 3pm CEST)- on Zoom Title: Greening your lab; Sustainable Practices & Benefits for your Life Science Lab Speakers: Julie Di Martino, New York Medical College, USA & Federico Forneris, University of Pavia, Italy

In a couple of days! Join the discussion from #TheMeshwork about Sustainable Practices and Benefits for #LifeScience labs. Happy to be part of this panel! @unipv @DBB_UniPV #BioReMediA @AlbertaPinnola #PNRR

Registration link: https://t.co/LGHaJW9XLH https://t.co/L2TXlb5BL4